滇黄芩化学成分的分离与鉴定

目的研究滇黄芩 (ScutellariaamoenaC .H .Wright)化学成分。 方法利用各种色谱技术进行分离纯化 ,根据理化性质和光谱数据进行结构鉴定。结果分离得到 8个化合物 ,分别鉴定为 :5 ,7 二羟基 6 甲氧基二氢黄酮 (dihydrooroxylinA ,Ⅰ )、5 ,7,4′ 三羟基 8 甲氧基黄酮 ( 5 ,7,4′ trihydroxy 8 methoxyflavone ,Ⅱ )、千层纸素A(oroxylinA ,Ⅲ )、汉黄芩素 (wogonin ,Ⅳ )、白杨素 (chrysin ,Ⅴ )、黄芩素 (baicalein ,Ⅵ )、去甲汉黄芩素 (norwogonine ,Ⅶ )、( 2S) 5 ,7,2′ ,6′ 四羟基二氢黄酮 [( 2S) 5 ,7,2′ ,6′ tetrahydroxyflavanone ,Ⅷ ]。 结论化合物Ⅰ、Ⅱ为该植物中首次分得     

Inhibition of Prostaglandin D2 Production by Trihydroxy Fatty Acids Isolated from Ulmus davidiana var. japonica

The stem and root barks of Ulmus davidiana var. japonica (Ulmaceae) have been used to treat inflammatory diseases including mastitis, rhinitis, sinusitis, and enteritis. In an ongoing study focused on the discovery of natural anti‐inflammatory compounds from natural products, a methanol extract of the stem and root barks of U. davidiana var. japonica showed anti‐inflammatory activities. Activity‐guided fractionation of the methanol extract yielded a new trihydroxy fatty acid, 9,12,13‐trihydroxyoctadeca‐10(Z),15(Z)‐dienoic acid (1), and a known compound, pinellic acid (2). These two trihydroxy fatty acids 1 and 2 inhibited prostaglandin D₂ production with IC₅₀ values of 25.8 and 40.8 μM, respectively. These results suggest that 9,12,13‐trihydroxyoctadeca‐10(Z),15(Z)‐dienoic acid (1) and pinellic acid (2) are among the anti‐inflammatory principles in this medicinal plant. Copyright © 2012 John Wiley & Sons, Ltd.     

Antioxidant and free radical scavenging activities of 5,7,3'-trihydroxy-3,6,4'-trimethoxyflavone from Brickellia veronicaefolia

The antioxidant and antiradical activities of 5,7,3'-trihydroxy-3,6,4'-trimethoxyflavone or centaureidin isolated and characterized from Brickellia veronicaefolia were elucidated by heat-induced oxidation in a beta-carotene and linoleic acid system and by the 1,1-diphenyl-2-picrylhydrazyl decoloration test. The centaureidin (32.1%) exhibited antioxidative activity less than that of BHT (95.5%) and alpha-tocopherol (95.9%) on oxidation in a beta-carotene and linoleic acid system. A moderate antiradical effect (47.6%) compared with BHT (96.7%) and alpha-tocopherol (94.6%) in DPPH decoloration test was found.     

中药北刘寄奴的化学成分研究

 目的从中药北刘寄奴中分离鉴定生物活性成分。方法溶剂提取及柱色谱法分离，光谱技术鉴定结构。结果分离得到7个化合物，分别鉴定为β-谷甾醇(Ⅰ)、7-甲氧基香豆素(Ⅱ)、7-羟香豆素(Ⅲ)、异阿魏酸(Ⅳ)、1R,2R,4R三羟基薄荷烷(Ⅴ)、反式对羟基桂皮酸(Ⅵ)和胡萝卜苷(Ⅶ)。结论7个化合物均为首次从该属植物中分得。     

The application of high pressure liquid chromatography to the analysis of bile salts in human bile.

Two high pressure liquid chromatography (HPLC) systems have been evaluated for the separation of conjugated bile salts from human bile. Partisil-10 ODS with mobile phase of methanol/water pH 2 (55 : 45) at 200 nm, 0.1 AUF UV detection gave only partial separation of bile salts. However, a mu Bondapak fatty acid analysis column using isopropanol/8.8 mmol/l potassium phosphate pH 2.5 (32 : 68) as the mobile phase and 193 nm, 0.1 AUF UV detection separated all the six conjugated bile salts in bile. The limit of detection ranged from 0.1 microgram for sodium taurocholate to 0.2 microgram for sodium glycodeoxycholate. The reproducibility and the application of the method to the analysis of conjugated bile salts was demonstrated using bile from five patients. Its application to the studies of hepato-biliary disease is discussed.     

无带水剂合成没食子酸丙酯新工艺

本研究采用无带水剂的新工艺，以非氧化性酸替代浓硫酸作催化剂、采用梯度升温法，使正丙醇与没食子酸在较高温度下作用生成没食子丙醇。通过正交试验探讨了反应温度、反应时间、催化剂用量和正丙醇用量对反应收率的影响，确定了最佳的工艺条件，收率达９４％。