佛手茎尖玻璃化超低温保存和植株再生

目的 为佛手种质资源的保存提供一条新途径.方法 对佛手茎尖进行了玻璃化超低温保存,并对保存后的茎尖进行微嫁接试验,获得再生植株.结果 佛手茎尖在含5%DMSO和5%蔗糖的MS培养基上预培养48h后,切取3～4 mm的茎尖,室温(25℃)下装载液(60%PVS_2)预处理30 min,0℃下玻璃化液PVS_2处理80 min,投入液氮保存24 h后取出,在40℃水浴快速化冻,室温下分别用1.2 mol/L蔗糖和MT基本培养基洗涤2次,每次10 min.保存后的茎尖经TTC法检测,成活率达81.25%;将茎尖嫁接到黑暗培养基的砧木上,再生率可达52.94%,且再生植株生长和分化正常,经PCR检测不含黄龙病病原.结论 玻璃化超低温保存方法可用于佛手种质资源保存.     

Prospective observational study comparing burn surgeons’ estimations of wound healing after skin grafting to photo-assisted methods

IntroductionAppropriate graft healing after split-thickness skin graft and early recognition of complications (graft loss) are critical to burn patient management. Larger mesh ratio expansions and Meek micrografting may pose a greater challenge in estimating the percentage of wound healing. This study looks at the reliability of photograph assessments and the concordance of bedside evaluation to photograph assessments of wound healing after skin grafting.MethodsThree assessment methods for percentage of wound healing after skin Grafting were assessed: (1) clinicians’ bedside rating, (2) clinician assessment of high-definition photographs, and (3) digital image analysis through color subtraction using Adobe Photoshop. We compared each method using a mixed-effects model on absolute agreement using intra-class correlation (ICC) and Bland Altman (BA) plots.ResultsFourteen burn patients were enrolled with 38 grafted wounds (100 sites). Bedside assessments had a mean ICC of 0.64 (compared to digital image analysis) and 0.69 (compared to photo assessment), with a wide range on BA-plots. Inter-rater reliability of photo assessment was excellent (0.96) among six clinicians. Repeated photo-assisted assessments had good intra-rater reliability (ICC: photo assessment: 0.88; digital analysis: 0.97).ConclusionsBedside wound healing assessments show variability; photograph documentation of sequential wound progression could supplement active clinical management or studies for more reliable assessments.     

Foot Technology, Part 1 of 2: The Micrograft Concept for Wound Healing: Strategies and Applications

The standard of care for wound coverage is to use an autologous skin graft. However, large or chronic wounds become an exceptionally challenging problem especially when donor sites are limited. It is important that the clinician be aware of various treatment modalities for wound care and incorporate those methods appropriately in the proper clinical context. This report reviews an alternative to traditional meshed skin grafting for wound coverage: micrografting. The physiological concept of micrografting, along with historical context, and the evolution of the technique are discussed, as well as studies needed for micrograft characterization and future applications of the technique.     

茎尖微嫁接技术脱除佛手病原体的研究

目的采用茎尖微嫁接技术脱除佛手病原体,为无病苗木的培育奠定基础。方法采用茎尖微嫁接技术获得了佛手微嫁接成活苗,应用PCR技术对其进行黄龙病病原进行检测,同时考察了茎尖大小对嫁接成活率和脱病原体率的影响。结果经PCR检测,微嫁接苗脱除了黄龙病病原;茎尖大小对嫁接成活率和脱病原体率的影响较大,带4个叶原基的茎尖嫁接成活率最高,达60.00%,脱病原体率为85.71%,带2个叶原基的茎尖,脱病原体率达100%,但嫁接成活率低。结论茎尖微嫁接技术可以脱除佛手病原体,综合考虑嫁接成活率和脱病原体的效果,宜选用带3～4个叶原基的茎尖进行脱病原体苗的生产。     

The tumescent technique facilitates hair micrografting

Micrografting for hair restoration has become increasingly popular because of the natural results it can achieve. The procedure is often performed under local anesthesia or local anesthesia with sedation. To achieve adequate anesthesia, supraorbital and supratrochlear nerve blocks are often performed as well as infiltration of the donor and recipient areas via multiple needle injections. These nerve blocks and multiple injections are associated with significant discomfort and may need to be repeated if anesthesia is inadequate. In addition, because of the rich vascularity of the scalp, donor site and recipient bed bleeding are often encountered, making the procedure more difficult and increasing postoperative bruising and morbidity. In an attempt to alleviate these difficulties and increase patient comfort, the tumescent technique was employed in 50 consecutive micrografting procedures. The ability to achieve anesthesia in the donor and recipient beds without the need for additional nerve blocks was evaluated. The ability of the tumescent technique to minimize bleeding and subsequent postoperative ecchymosis and morbidity was analyzed. The findings of this study suggest the tumescent technique is useful in facilitating hair micrografting. It consistently achieves excellent anesthesia in both donor and recipient sites without the need for multiple needle injections or nerve blocks. The duration of anesthesia is significant, allowing completion of the procedure and offering prolonged postoperative anesthesia as well. Bleeding can be minimized using the tumescent technique for both donor site harvesting and the micrografting procedure itself.     

佛手茎尖微嫁接技术研究

目的探讨佛手茎尖微嫁接技术,为佛手无病苗木的培育奠定基础。方法黑暗条件下培养柠檬实生苗用作砧木,以佛手茎尖为接穗,采用倒"T"字形法进行茎尖微嫁接试验,并对影响嫁接成活率的因素进行研究。结果成功获得了佛手茎尖微嫁接苗,并优化了微嫁接的条件:选取苗龄为14d的柠檬实生苗为砧木,嫁接成活率较高;以带4个叶原基的佛手茎尖为接穗,效果较好,嫁接成活率可达65.2%;茎尖微嫁接后,切口外缠parafilm膜,能明显提高成活率。结论建立了佛手茎尖微嫁接的方法,获得了嫁接成活苗。     

怀地黄茎尖培养和植株再生技术的研究

目的　研究怀地黄茎尖培养技术 ,为怀地黄茎尖脱毒苗建立最佳培养条件。方法　利用植物组织培养技术 ,将怀地黄茎尖分别在不同组合的培养基上进行培养 ,诱导其成为完整植株。结果　怀地黄茎尖在MS +6 -BA0 .3mg·L-1+NAA0 .0 2mg .L-1+GA0 .1mg·L-1培养基上能生长成健壮苗 ;在MS +PP3 3 3 1培养基上能生成健壮根系 ,长成完全小植株。结论　利用怀地黄茎尖在合适的培养基上 ,能再生出完整的植株     

Meek微型皮片移植术中双皱扩展纱布的选择

目的探讨Meek微型皮片移植术（下称M法）在修复烧伤创面中不同规格的双皱纱布（下称纱布）在术前数量的选择。方法测量患者一侧手掌平面的面积（1％）及皮片受区的面积，应用数学方法筛选不同规格纱布的数量。结果14例采用此法筛选，纱布数量准确；13例受区在术后2～3周皮片融合上皮化；1例术后25d上皮化。结论应用数学方法在术前或术中筛选纱布数量适用于任何大小面积的植皮创面。     

佛手茎尖微嫁接技术研究

目的探讨佛手茎尖微嫁接技术,为佛手无病苗木的培育奠定基础。方法黑暗条件下培养柠檬实生苗用作砧木,以佛手茎尖为接穗,采用倒"T"字形法进行茎尖微嫁接试验,并对影响嫁接成活率的因素进行研究。结果成功获得了佛手茎尖微嫁接苗,并优化了微嫁接的条件:选取苗龄为14d的柠檬实生苗为砧木,嫁接成活率较高;以带4个叶原基的佛手茎尖为接穗,效果较好,嫁接成活率可达65.2%;茎尖微嫁接后,切口外缠parafilm膜,能明显提高成活率。结论建立了佛手茎尖微嫁接的方法,获得了嫁接成活苗。     

半夏脱毒技术研究及应用

目的:通过脱毒快繁技术解决半夏因体内病毒积累导致种性退化和品质降低的问题。方法:以半夏叶、叶柄为外植体,采用高温处理(38℃,40 d)结合茎尖培养对半夏进行脱毒技术研究。结果:高温处理40 d左右,88.9%的植株叶片不带斑点;利用1.0 mg/L 6-BA、0.1 mg/L NAA诱导茎尖成苗,成苗率可达91.4%;MS附加0.5mg/L 6-BA、0.1 mg/L NAA有利于试管苗生长,附加0.5 mg/L KT、0.5 mg/L NAA诱导形成的单株平均根数和根长指标最好,移栽成活率可达89.5%。结论:建立了可靠的半夏脱毒快繁技术体系。