Enhancing and suppressive effects of tumour necrosis factor/cachectin on growth of Trypanosoma musculi

The effect of human recombinant TNF on the growth of T. musculi has been investigated. When added to parasites cultured in vitro, TNF inhibited their growth. In the presence of thioglycollate-elicited peritoneal exudate cells, the opposite effect was seen and TNF enhanced the growth of trypanosomes in vitro. Similarly, administration of TNF in vivo during the course of infection led to a net increase in the parasite population. It is suggested that TNF exerts a direct antitrypanosomal effect while simultaneously promoting the growth of the parasite through an indirect effect mediated via the host's cells, possibly the macrophages.     

枸杞多糖对运动性骨骼肌疲劳恢复的细胞定量化学分析和电镜观察

通过小鼠实验，用枸杞多糖解除运动性骨骼肌疲劳，以显微分光光度计进行琥珀酸脱氢酶(SDH)和糖原的定性定量分析，并于透射电镜下观察超微结构的变化。结果显示，运动疲劳(B)组糖原含量明显降低，各类肌纤维与对照(A)组比较P<0．001。SDH活性增加，各类肌纤维与A组比较P<0．001。服用枸杞多糖(C)组糖原含量增加，各类肌纤维与A组之较P<0．001；与B组比较P<0．001；SDH反应较B组更深，各类肌纤维与A组比较P<0．001，若与B组比较，则其中Ⅰ(慢肌)型和Ⅱ(中间肌)型P<0．001，而Ⅱ(快肌)型则P>0．05。超微结构显示，B组线粒体增多且体积明显增大，呈肿胀状态，C组全无此类改变，提示服用枸杞多糖后可解除疲劳，其中以Ⅰ(慢肌)型肌纤维效果尤佳。     

封闭群子午沙鼠寄生虫感染的研究及其药物净化

[摘要] 目的 了解子午沙鼠种群寄生虫感染状况,并有针对性选择药物及合适剂量驱除子午沙鼠体内外寄生虫,使子午沙鼠得到净化,为建立清洁级子午沙鼠种群奠定基础,为制定子午沙鼠寄生虫检测标准提供参考依据。方法 对封闭群子午沙鼠寄生虫感染情况进行调查研究,选择胺菊?氯菊酯、吡喹酮、替硝唑三种药物,分为高、中、低剂量对子午沙鼠用药,观察子午沙鼠体内外寄生虫驱除情况。结果 在子午沙鼠体内检出鼠三毛滴虫和短膜壳绦虫两种体内寄生虫,体外寄生虫有鼠肉螨。高、中、低剂量组能不同程度杀死子午沙鼠体表食肉螨,鼠肉螨用1％的胺菊?氯菊酯药浴子午沙鼠三次即可驱除子午沙鼠体表的鼠肉螨,有螨动物只数为零。吡喹酮中剂量组（31mg/kg）有完全杀死子午沙鼠体内短膜壳绦虫的作用,且为最佳给药剂量。替硝唑中剂量组（42mg/kg）为净化子午沙鼠体内鼠三毛滴虫的最佳剂量。结论 现有的子午沙鼠体内有鼠三毛滴虫和短膜壳绦虫感染,体外有鼠肉螨寄生,三种抗寄生虫药物具有选择性驱除这些寄生虫的作用。     

西宁地区保留胸肌开胸方法的应用体会

目的比较两种开胸手术方式的优缺点.方法回顾分析传统开胸、腋下小切口开胸治疗气胸及肺周围性良性肿瘤（共47例）的疗效. 结果两组术后比较腋下组较传统组在缩短手术时间、带管天数、引流量、杜冷丁用量、住院日及保持肺功能上均优先. 结论腋下小切口手术符合微创的要求,适应于开胸手术.传统开胸手术创伤大、恢复慢,其适应症较宽,但对病情复杂病例有不可替代作用.     

耻骨直肠肌部分切除治疗耻骨直肠肌综合征1 49例

目的观察耻骨直肠肌部分切除术的治疗效果.方法149例经直肠排粪造影和耻骨直肠肌肌电图确诊为耻骨直肠肌综合征患者,经尾骨下正中切口行耻骨直肠肌部分切除术,术后观察其临床症状改善情况,复查直肠排粪造影了解其影像学变化.结果手术后排便困难均得到完全缓解,肛门坠胀或疼痛有15例未完全消除,但程度明显减轻;直肠排粪造影显示耻骨直肠肌压迹消失.结论耻骨直肠肌部分切除术治疗耻骨直肠肌综合征方法简便,疗效好,并发症少.     

正常胸大肌的超声图像及临床研究

目的 正确识别健康人胸大肌声像图,为其病变诊断提供依据。方法 通过高频超声检查健康人正常胸大肌,及猪离体新鲜运动肌水槽内超声检测。结果 结合组织学结构确认肌肉声像图特征,动态变化以及血流动力的基础参数。结论 提供正常胸大、小肌声像图的形态、大小、肌肉的回声,正常胸大肌的超声图像及数值,可作为病变诊断的基础参数。     

额肌瓣悬吊治疗重度上睑下垂

上睑下垂有先天性和后天性,单侧和双侧。由于睑上提肌功能减弱或消失,导致上睑缘部分或全部遮盖瞳孔,影响视力及外形,以致在张眼时,患者常以抬眉皱额头部后仰加以代偿。临床上治疗方法有多种,各有特点。本文利用额肌瓣转移治疗不同类型的上睑下垂30例,在原手术方法的基础上加以改进,不切开额肌的外侧缘,不打开眶隔,剥离范围小,出血少,手术操作简便,效果可靠等优点。     

Thymus graft reconstitution of T-cell-deprived mice infected with Trypanosoma musculi

T-cell-deprived CBA mice were given thymus graft implants during the course of an infection with Trypanosoma musculi and at a time when the parasitaemia was high. As a consequence of the grafts, most of the mice survived the infection whereas control ungrafted mice died. In grafted animals there was generally a period of 6&80 days after grafting during which time parasitic numbers remained high, and a further period of 200 or more days when trypanosomes were still detectable in the circulation. The mice were highly variable in their responses and possible reasons for this are considered.     

Ablastin and the control of Trypanosoma musculi infections in mice

Trypanosoma musculi parasites were grown in vitro in the presence of sera from intact or T-cell-deprived infected mice. Parasite division was not inhibited by the presence of the antisera, but the total number of young parasites produced was lower than expected as there was thus no evidence for the presence of ablastin in the sera, only a trypanocidal effect. The implications for in vivo control of the infection are discussed.     

鹰爪豆碱对动物横纹肌作功和肌电的影响

鹰爪豆碱10～20mg/kg静脉给药,明显增加麻醉兔胫前肌收缩力和作功,并能维持30min,显著延长小鼠游泳时间;明显升高麻醉兔胫前肌诱发电位。