艾绒的化学成分研究

目的 对艾绒的化学成分进行研究。方法 采用硅胶、ODS、MCI、Sephadex LH-20和prep HPLC等现代色谱技术对其化学成分进行分离纯化,利用MS、NMR等现代波谱技术鉴定单体化合物结构;采用脂多糖（lipopolysaccharide,LPS）诱导的小鼠巨噬细胞RAW264.7炎症模型,开展新化合物的抗炎活性评价。结果 从艾绒醋酸乙酯提取物中分离得到13个化合物,分别鉴定为 (4S,5S)-5-[(R)-5''-hydroxy-2''-methyl-3''-oxocyclopent-1''-en-1''-yl]-3-methylene-4-(3-oxobutyl)dihydrofuran-2(3H)-one（1）、iso-seco-tanapartholide（2）、3-methoxy-tanapartholide（3）、3-acetyl-iso-seco-tanapartholide（4）、甲基条叶蓟素（5）、圣草酚（6）、泽兰黄素（7）、半齿泽兰素（8）、茵陈色原酮（9）、矢车菊黄素（10）、柚皮素（11）、高圣草酚（12）、异泽兰黄素（13）。化合物1能够抑制LPS诱导的RAW264.7细胞中NO的生成量,半数抑制浓度（median inhibition concentration,IC₅₀）值为（31.15±2.30）μmol/L。结论 化合物1为新化合物,并命名为1R-长叶艾菊内酯,化合物4为首次从菊科植物中分离得到;化合物1具有一定的抗炎活性。     

高圣草酚衍生物的合成及其生物活性的初步研究

槲寄生水提取液制成的注射液对冠心病引起的心绞痛有较好的疗效。现以其甙元高圣草酚为先导化合物,合成了具有不同酸、碱性侧链的氨基酸和二肽衍生物。初步药理结果表明,在相同剂量下,所有氨基酸和二肽衍生物(8a～f)对 ADP 诱导的兔血小板聚集的抑制作用均比甙元显著增强。其中,赖氨酸(3e(?)和甘氨酰丙氨酸的衍生物(3f)当剂量为0.54 μmol/ml 时,抑制率可达100%,而甙元在此剂量则无效。     

Sweetener influences plasma concentration of flavonoids in humans after an acute intake of a new (poly)phenol-rich beverage

Background and aimThe overconsumption of sucrose is closely related to sugar-sweetened beverages and one of the main factors associated with the increase of metabolic diseases, such as type 2 diabetes, obesity, and insulin resistance. So, the addition of alternative sweeteners to new fruit-based drinks could contribute to minimizing the incidence or severity of these pathologies. Nevertheless, current knowledge on the influence of these additives on the bioactive compounds present in these beverages is still scarce.new-onset hypertension, but few data were published in Asian. We aimed to investigate the association of lipid profiles with new-onset hypertension in a Chinese community-based non-hypertensive cohort without lipid-lowering treatment (n = 1802).Methods and resultsHence, to contribute to the understanding of this issue, the plasma concentration of phenolic compounds (anthocyanins and flavanones), after the ingestion of a new maqui-citrus-based beverage, supplemented with sucrose (natural high caloric), stevia (natural non-caloric), or sucralose (artificial non-caloric), was evaluated as evidence of their intestinal absorption and metabolism previous to renal excretion. The beverages were ingested by volunteers (n = 20) and the resulting phenolic metabolites in plasma were analyzed by UHPLC-ESI-MS/MS. A total of 13 metabolites were detected: caffeic acid sulfate, caffeic acid glucuronide, 3,4-dihydroxyfenylacetic, 3,4-dihydroxyfenylacetic sulfate. 3,4-dihydroxyfenylacetic acid di-sulfate, 3,4-dihydroxyfenylacetic di-glucuronide, 3,4-dihydroxyfenylacetic glucuronide-sulfate, trans-ferulic acid glucuronide, naringenin glucuronide, vanillic acid, vanillic acid sulfate, vanillic acid glucuronide-sulfate, and vanillic acid di-glucuronide, being recorded their maximum concentration after 30–60 min.ConclusionIn general, sucralose provided the greatest absorption value for most of these metabolites, followed by stevia. Due to this, the present study proposes sucralose and stevia (non-caloric sweeteners) as valuable alternatives to sucrose (high caloric sweetener), to avoid the augmented risk of several metabolic disorders.     

高圣草素及其B环异构物的合成和结构特征

制备了高圣草素及其 B 环羟基与甲氧基取代位置不同的黄烷酮共6个。经药理初筛,其中5个显示一定的抑制血小板聚集和增加冠脉流量的作用。     

黄花蒿中1个新的香豆素苷类化合物

目的研究黄花蒿Artemisia annua全草大极性部位的化学成分。方法采用Sephadex LH-20柱色谱、硅胶柱色谱、ODS柱色谱和制备薄层色谱等方法进行分离纯化,根据波谱数据确定化合物的结构。结果从黄花蒿70%乙醇冷浸提取物的甲醇可溶部位中共分离得到14个化合物,分别鉴定为黄花蒿苷A(1)、东莨菪苷(2)、2,4-二羟基-6-甲氧基苯乙酮-4-O-β-D-吡喃葡萄糖苷(3)、咖啡酸甲酯(4)、水杨酸(5)、阿魏酸(6)、香草酸(7)、2,4-二羟基-6-甲氧基苯乙酮(8)、高圣草酚(9)、棕鳞矢车菊黄酮素(10)、penduletin(11)、甲氧基寿菊素(12)、圣草酚(13)和(R)-15,16-didehydrocoriolic acid(14)。结论化合物1为未见文献报道的新香豆素苷;化合物14为首次从蒿属植物中分离得到;化合物9为首次从黄花蒿中得到。     

珍珠莲中的黄酮类化学成分

目的 研究珍珠莲Ficus sarmentosa var.henryi中的黄酮类化学成分.方法 应用柱色谱技术分离纯化.核磁共振及质谱鉴定化合物结构.结果 从珍珠莲氯仿和醋酸乙酯层萃取物中分离得到11个黄酮类化合物,分别鉴定为圣草酚(eriodictyol,1)、高圣草酚(homoeriodictyol,2)、槲皮素(quercetin,3)、二氢槲皮素(dihydroquercetin,4)、山柰酚(kaempferol,5)、二氢山柰酚(dihydrokaempferol,6)、柚皮素(naringenin,7)、木犀草素(luteolin,8)、芹菜素(apigenin,9)、柯依利素(chrysoeriol,10)、3',5',5,7-四羟基二氢黄酮(3',5',5,7-tetrahydroxyl-favanone,11).结论 化合物1～11均为首次从该植物中分离得到.