排序方式: 共有28条查询结果,搜索用时 15 毫秒
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目的探讨防己诺林碱对去卵巢小鼠骨质疏松的保护作用。方法将40只C57BL/6小鼠随机分为空白(假手术)对照组、模型(去卵巢)组、阳性(雌二醇,E2)对照组、防己诺林碱组共4组,每组10只。连续腹腔给药7周后处死小鼠,取股骨及外周血清,通过ELISA法检测骨代谢相关血清学指标:抗酒石酸酸性磷酸酶(TRAc P)、I型胶原羧基末端肽(CTX)及I型胶原氨基末端肽(NTX);通过Micro-CT评估各组小鼠骨组织微结构相关指标:骨小梁百分比(BV/TV)、骨小梁数量(Tb.N)、骨小梁分离度(Tb.Sp)和骨小梁厚度(Tb.Th);通过实时荧光定量PCR检测小鼠骨质疏松骨吸收相关基因的表达情况,包括TRAc P、组织蛋白酶K(Cathepsin K)、活化T细胞核因子1(NFATc1)以及降钙素受体(CTR)。结果与去卵巢模型组比较,防己诺林碱治疗组小鼠BV/TV、Tb.N、Tb.Th显著升高,Tb.Sp明显降低,骨代谢相关指标(TRAc P、CTX、NTX)显著降低,破骨细胞标志基因(TRAc P、Cathepsin K、NFATc1以及CTR)表达水平明显下降。结论防己诺林碱对去卵巢小鼠骨质疏松有保护作用,有望为骨质疏松的临床用药提供新思路。 相似文献
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小续命汤有效成分组中防己诺林碱和粉防己碱在大鼠体内药动学研究 总被引:4,自引:0,他引:4
目的:对防己诺林碱与粉防己碱两种化合物在大鼠体内的药动学及复方给药的影响进行研究.方法:采用高效液相色谱-质谱联用技术,建立了防己诺林碱与粉防己碱的血药浓度检测方法,并对单体给药与小续命汤有效成分组给药两种情况下两个化合物的药动学参数进行对比.结果:与有效成分组给药相比,单体给药防己诺林碱与粉防己碱的T_(max)显著延长,其余参数没有大的差异.结论:有效成分组中其他成分减慢了二者在体内的吸收速度,与单体给药相比,有效成分组给药时防己诺林碱与粉防己碱的生物利用度不变. 相似文献
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目的测定中肺合剂中防己诺林碱、粉防己碱的含量。方法样品经预处理后采用RP-HPLC同时测定它们的含量。色谱条件:色谱柱为Agilent Zorbax Elipse XDB-C18;流动相为0.06%三乙胺-乙腈溶液(44∶56);流速为1.0 mL·min-1;检测波长设在240 nm;柱温30℃。结果防己诺林碱在0.437 5~17.5 mg·L-1与粉防己碱在0.875~35.0 mg·L-1内,峰面积与其浓度具有良好的线性关系。平均加样回收率分别为89.30%~94.35%(防己诺林碱),90.08%~95.74%(粉防己碱)。结论本方法简便、快速、准确,可同时测定中肺合剂中防己诺林碱和粉防己碱的含量。 相似文献
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Cardiovascular actions of Radix Stephaniae Tetrandrae: a comparison with its main component, tetrandrine 总被引:6,自引:2,他引:4
A comparison of the cardiovascular actions of the extract of Radix Stephaniae Tetrandrae (RST), the root of a Chinese herb Stephania tetrandra S Moore, in rats with those of tetrandrine (Tet), the best known active component of RST was reviewed. The RST extract inhibits Ca2 influx into the myocyte and reduces protein release during reperfusion with a Ca2 containing solution following perfusion with a Ca2 free solution (Ca2 paradox) , and arrhythmia during reperfusion in the isolated perfused heart. It also reduces the infarct size induced by ischemia/reperfusion in vitro and in vivo. In addition, the RST extract suppresses elevation of arterial blood pressure in DOCA-salt hypertensive rats. It does not further reduce the heart rate and coronary flow significantly during myocardial ischemia. The effects are similar to those of Tet. When compared with the same doses of Tet alone, the RST extract, of which 9 % is Tet, produces equally potent effects on infarction, arrhythmias, coronary flow and heart r 相似文献
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Fangchinoline (FAN; a plant alkaloid isolated from Stephania tetrandrae) is a nonspecific Ca(2+) channel blocker. The objective of the present study was to investigate the effect of FAN on the growth factor-induced proliferation of primary cultured rat aortic smooth muscle cells (RASMCs). FAN significantly inhibited both 5% fetal bovine serum (FBS)- and 50ng/mL platelet-derived growth factor (PDGF)-BB-induced proliferation, [3H]thymidine incorporation into DNA and phosphorylation of extracellular signal-regulated kinase 1/2. In accordance with these findings, FAN revealed blocking of the FBS-inducible progression through G(0)/G(1) to S phase of the cell cycle in synchronized cells and caused a 62% decrease in the early elevation of c-fos expression induced after 5% FBS addition. Furthermore, significant antiproliferative activity of FAN is observed at concentrations below those required to achieve significant inhibition of Ca(2+) channels by FAN. These results suggest that FAN reduced both FBS- and PDGF-BB-induced RASMCs proliferation by perturbing cell cycle progression. This antiproliferative effect of FAN is dependent on the MAP kinase pathway, but cannot be limited to its Ca(2+) modulation. 相似文献
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目的 建立梯度洗脱联合波长切换HPLC法同时测定稀莶风湿丸中稀莶苷、奇壬醇、稀莶精醇、防己诺林碱和粉防己碱.方法 采用Kromasil C18色谱柱(4.6 mm× 250 mm,5μm),以流动相A:乙腈-甲醇(2∶1)、流动相B:0.05%冰醋酸溶液进行梯度洗脱;流速为1.0 mL/min;样量为20 μL;豨莶苷、奇壬醇和稀莶精醇检测波长为215 nm,防己诺林碱和粉防己碱检测波长为280 nm.结果 豨莶苷、奇壬醇、稀莶精醇、防己诺林碱和粉防己碱在给定浓度范围内的线性范围分别为6.45~129.00 μg/mL(r=0.999 5)、5.61~112.20 μg/mL(r=-0.999 9)、4.25~85.00 μg/mL(r=0.999 3)、9.19~183.80 μg/mL(r=0.999 8)和11.05~221.00 μg/mL(r=0.999 7),线性关系良好;平均加样回收率和相对标准偏差(RSD)值分别为98.73%(1.43%)、97.63%(1.28%)、99.44%(1.29%)、98.33%(1.38%)、97.36% (1.37%).结论 建立的梯度洗脱联合波长切换HPLC法能同时测定豨莶风湿丸中稀莶苷、奇壬醇、稀莶精醇、防己诺林碱和粉防己碱含量,方法简便、准确、重现性好,为稀莶风湿丸质量控制提供了可靠方法. 相似文献
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目的 建立豨莶风湿片中粉防己碱及防己诺林碱的HPLC测定方法.方法 色谱柱为C<,18>柱(4.6mm×l50 mm,5 μm);流动相为乙腈-水-三乙胺(70:30:0.01),流速为1.0 mL/min,检测波长为280nm.结果 粉防己碱及防已诺林碱的进样量分别在0.344 μg~1.72 μg(r=0.999 ... 相似文献
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HPLC法测定防已黄芪颗粒剂中防已诺林碱、粉防已碱的含量 总被引:2,自引:0,他引:2
目的:测定防已黄芪颗粒剂中防已诺林碱,粉防已碱的含量,方法:采用反相高效液相色谱法,结果:防已黄芪颗粒剂中已诺林碱,粉防已碱的含量,平均加样回收率分别已诺林碱:98.2%,RSD0.84%,粉防已碱:96.9%,RSD为1.45%,结论:本法简单,灵敏,结果可靠,可作为防已黄芪颗粒剂质量控制的定量方法。 相似文献
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