中药大蜜丸中活螨快速分离方法

本文对极易污染活螨的中药大蜜丸,在应用分离法的基础上,根据螨怕光、怕热的习性,适当调整光照,周围严格避光,达到分离活螨的目的.     

Effect of atropine on the bronchial response of asthmatic subjects to the inhalation of ultrasonically nebulized distilled water

To determine whether atropine provides protection against the bronchoconstriction that develops in asthmatic subjects after inhalation of ultrasonically nebulized distilled water, we exposed six asthmatic patients to this stimulus with and without pretreatment with atropine (0.04 mg/kg). The mean FEV1 decreased from 3.32 to 2.39 L (-28%) without and from 3.49 to 3.18 L (-9%) with atropine. This protective effect was statistically significant (p less than 0.05), suggesting that cholinergic pathways are involved in the obstructive response to the inhalation of ultrasonically nebulized distilled water.     

异丙酚复合芬太尼维持麻醉对脑代谢的影响

观察异丙酚复合芬太尼维持麻醉对脑代谢的影响。方法： 择期手术病人８ 例, 芬太尼、硫贲妥钠、维库溴铵诱导插管, 静脉联接Ｇｒａｓｅｂｙ 微泵异丙酚８ ｍｇ／ （ｋｇ·ｈ） , 芬太尼１ μｇ／ （ｋｇ·ｈ）维持麻醉。连续监测ＭＡＰ、心电图、脉搏氧饱和度和呼气末二氧化碳分压, 同步采集动脉血和颈内静脉血作血气分析, 计算动－ 静脉氧含量差（Ｄａ － ｖＯ２） 和脑氧摄取率（ＥＲＯ２） 。结果： 异丙酚麻醉维持平稳, Ｄａ － ｖＯ２ 和ＥＲＯ２ 在给药３０ ｍｉｎ 、６０ ｍｉｎ 较气管插管后５ ｍｉｎ 无明显改变, 有平均动脉压下降和心动过缓。结论： 异丙酚复合芬太尼维持麻醉可保持脑氧供需平衡稳定。     

蒸馏水与其化疗药物溶液对胃癌MGC-803细胞作用1分钟的灭活效果比较

目的：观察常温蒸馏水、温热蒸馏水和常温蒸馏水加不同化疗药的溶液短时间（1分钟）作用于胃癌细胞MGC-803的结果，从中筛选出能杀灭癌细胞的最低冲洗浓度，为临床胃癌术野的蒸馏水化疗药物冲洗提供实验依据。方法：选取常见胃癌细胞MGC-803为实验对象，以蒸馏水（DW）、43℃温热蒸馏水（HDW）和常用化疗药阿霉素（ADM）、顺铂（DDP）、5-氟尿嘧啶（5-FU）与蒸馏水混合溶液作为干预方法，分别以不同浓度（DW＋ADM/DDP/5-FU：10/10/50μg/ml、20/20/100μg/ml、40/40/200μg/ml、80/80/400μg/ml、160/160/800μg/ml、320/320/1600μg/ml、640/640/3200μg/ml）的水溶液作用细胞1分钟并用培养液继续培养72小时后进行细胞形态学及MTT实验。结果：蒸馏水化疗药溶液相对于单纯蒸馏水和温热蒸馏水对胃癌细胞具有更强的杀伤作用，结果具有统计学意义（P＜0．01）；筛选出3种化疗药物的最低完全抑制浓度（抑制率＞90％），分别为：ADM 160μg/ml、DDP 320μg/ml、5-FU 1600μg/ml。结论：蒸馏水化疗药溶液相比于单纯蒸馏水及温热蒸馏水可明显增加对胃癌细胞的杀伤作用，筛选出的蒸馏水化疗药溶液最低浓度可对临床胃癌手术以及其他癌症手术术野冲洗液的选择提供一定的实验依据。     

Sustained Release of Minocycline From Minocycline-Calcium-Dextran Sulfate Complex Microparticles for Periodontitis Treatment

It is important to address the periodontitis-associated bacteria in the residual subgingival plaque after scaling and root planing to successfully treat periodontitis. In this study, we explored the possibility of exploiting the ion pairing/complexation of minocycline, Ca²⁺, and sulfate/sulfonate-bearing biopolymers to develop an intrapocket delivery system of minocycline as an adjunct to scaling and root planing. Minocycline-calcium-dextran sulfate complex microparticles were synthesized from minocycline, CaCl₂, and dextran sulfate. They were characterized using Fourier-transform infrared spectroscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. An in vitro release study was conducted to evaluate the release kinetics of minocycline from these microparticles. Agar disk diffusion assays and biofilm-grown bacteria assays were used to assess antibacterial capability. High loading efficiency (96.98% ± 0.12%) and high loading content (44.69% ± 0.03%) for minocycline were observed for these complex microparticles. Mino-Ca-DS microparticles achieved sustained release of minocycline for at least 9 days at pH 7.4 and 18 days at pH 6.4 in phosphate-buffered saline, respectively. They also demonstrated potent antimicrobial effects against Streptococcus mutans and Aggregatibacter actinomycetemcomitans in agar disk diffusion and biofilm assays. These results suggested that the ion pairing/complexation of minocycline, Ca²⁺, and sulfonate/sulfate-bearing biopolymers can be exploited to develop complex microparticles as local delivery systems for periodontitis treatment.     

新型氧气湿化输送系统的研制与临床应用

目的保证氧疗效果,解决氧气湿化过程中普遍存在的湿化液污染及湿化产生噪声问题。方法将172例需吸氧的肺癌患者随机分为观察组和对照组各86例,观察组应用自行研制的新型氧气湿化输送系统吸氧,对照组使用传统氧气湿化输送系统吸氧。结果观察组仅1例患者主观感受到湿化噪声,与对照组比较,差异有统计学意义（P〈0．01）;观察组湿化单元细菌培养阳性率显著低于对照组（P〈0．01）。结论新型氧气湿化输送系统应用于临床氧疗,能降低传统氧气湿化液污染导致的医院呼吸道感染的风险,降低噪声,可保证患者休息,且不影响氧疗效果。     

芦荟提取物C诱生小鼠肿瘤坏死因子的研究

目的 :研究芦荟提取物C(aloeextractC ,AE C)在BALB C鼠体内诱导产生肿瘤坏死因子 (tu mornecrosisfactor ,TNF)。方法 :连续 5d给小鼠注射AE C ,第 6天取血分离血清 ,应用结晶紫染色法测其TNF活性 ,观察经AE C诱生的鼠血清对多种瘤细胞的杀伤作用 ,杀伤作用的持续时间和对温度的敏感性及血清不同稀释度的杀伤效果。结果 :AE C能诱导小鼠产生TNF α ,与对照组比较作用明显 (P <0 0 1) ;并且对多种靶细胞有显著的杀伤作用 ;TNF活性 2 4h达高峰 ,第 4天下降明显 ,5d接近正常水平 ;血清标本稀释至 2 0 0倍时 ,仍具有较强的杀伤活性 ;诱生后的TNF有一定的温度敏感性。结论 :芦荟提取物C有诱生BALB C鼠产生肿瘤坏死因子的作用     

蒸馏水NO_3与NO_2杂质检查假阳性结果的成因及防止

 将中国药典（１９９０年版）蒸馏水中与杂质检查法倒置应用，首先检查了所用试剂（液）自身的致阳性状况，探明了诱发假阳性的４种可能因素：醋酸和冰醋酸的致假阳性率分别为６６．６％（４／６）和５０％（３／６）；采用合阳性杂庙的醋醚、冰醋酸或水（三者之一）所配成的Ｇｒｉｅｓｓ’ｓ液；锌粉过量；操作不当所致的污染。试验还证明，醋酸酐在未完全水解之前不仅不能代替醋醚，相反对与杂质检出有间接的掩蔽作用。     

蜘蛛香超临界CO2萃取物化学成分的研究

目的研究蜘蛛香的超临界CO2萃取物的化学成分及相对含量。方法采用超临界CO2萃取蜘蛛香挥发油,利用气相色谱-质谱联用技术(GC-MS)测定化学成分及相对含量;检索质谱库并参考相关文献进行鉴定。结果蜘蛛香超临界CO2萃取物得率为8.85%,分离得到80个组分峰,对其中的74种成分进行了鉴定分析。结论应用超临界CO2萃取蜘蛛香,提高了挥发油收率,最大限度地保留了天然组分,值得推广应用于蜘蛛香的研究开发和工业提取。