近红外漫反射光谱技术在甘草指纹图谱中的研究

本文采用光纤近红外漫反射光谱技术对不同产地、不同采收时间、不同级别、不同部位的甘草进行考察,通过非侵入方式获得植物内在成分信息,应用系统聚类分析方法获得分类结果,探索了NIRS在植物分类中的应用途径和方法。方法学研究表明,本法具有快捷、无损和重现性高地表达植物化学信息的特点,可作为中药分类中的一个强有力的工具。     

False and mycoplasma‐contaminated leukemia–lymphoma cell lines: time for a reappraisal

Leukemia–lymphoma cell lines are important research tools in a variety of fields. To represent adequate model systems it is of utmost importance that cell lines faithfully model the primary tumor material and are not cross‐contaminated with unrelated cell material (or contaminated with mycoplasma). As it has been previously reported that cross‐contaminated cell lines represent a significant problem, it is of interest to know whether any improvement in the prevalence of such “false cell lines” had occurred since we called the alert in 1999. A retrospective review of our data archives covered 848 cell lines received from 1990 to 2014 from 290 laboratories in 23 countries spanning the spectrum of leukemia–lymphoma entities. Two variables were considered: authenticity and freedom from mycoplasma infection. Regarding provenance, we separately considered primary sources (original investigators having established the cell lines or reference repositories) and secondary sources. The percentages of mycoplasma‐contaminated cell lines decreased significantly over the 25‐year timespan. Among primary sourced material: mycoplasma‐contamination fell from 23% to 0%; among secondary sourced: from 48% to 21%. The corresponding figures for cross‐contamination declined from 15% to 6%, while among material obtained from secondary sources prevalence remained remarkably high, throughout the time periods at 14–18%. Taken together, our data indicate that using non‐authenticated cell lines from secondary sources carries a risk of about 1:6 for obtaining a false cell line. The use of authentic leukemia–lymphoma cell lines holds important translational value for their model character and the reproducibility of the laboratory data in the clinical arena.