CD3+木瓜提取物对ApoE基因缺陷小鼠血脂及炎性相关因素的影响

目的观察木瓜提取物对ApoE基因缺陷小鼠血脂水平、主动脉窦斑块中巨噬细胞活化及肿瘤坏死因子(TNF-α)水平的影响。方法30只雄性ApoE基因缺陷小鼠随机分为3组:对照组、低剂量组和高剂量组,低剂量组和高剂量组分别在基础饲料上添加5%和10%的木瓜提取物喷雾干燥颗粒,饲养16周后经小鼠眼眶静脉取血后处死动物,酶法测定血脂水平、采用免疫组化分析主动脉窦斑块中TNF-α的表达水平和采用免疫荧光反映斑块CD68阳性表达水平与巨噬细胞阳性表达率。结果低剂量组和高剂量组的血清TC和LDL-C显著低于对照组(P<0.05),木瓜提取物能显著下调斑块中TNF-α的表达水平以及减少巨噬细胞的阳性率。结论木瓜提取物降低了血清脂蛋白胆固醇水平(TC和LDL-C),减少了炎性因子TNF-α表达以及减弱巨噬细胞参与程度,预示其可能具有减缓动脉斑块发生发展的抗动脉粥样硬化作用。     

TNF-α基因转染对胃癌细胞系的生长抑制效应

名誉主编Ill,llorarrbotoy-n-che吴盂超WuMe叱咖肋（fecorlrlMilitalyMhMUniversty,戳皿吟凶2（xM.--）学术顾问WAdfor巴德年BaDe。（hnesepofMhcaleiences,Betinglop30）刘新垣iulmp（孔皿吟dInstaofBbo,Cllllleseqofdences,砌吧饲2（XXB）吴妄Wuin（CancerInstitUte,ChineseA脚加阳OfMeicalde。,阿吨l（XXll）汤＄1JffiTpZhaoou（aMhcalUmversity,枷呷饲ZffX）32）主编itoY—n—cief张友会Zllazlzyouhni（CancelnstitUte,ClllxleseAcAsyofMwhedAs。,Betwl（XXll）副主编A田叨h扣hoot.勿一咖姓崔正言ClZhangan（Depe…     

痹症停合剂对大鼠佐剂性关节炎的防治作用及其机制研究

痹症停合剂是由雷公藤,赤芍,防风等10余味中药组成.临床主要用于治疗类风湿关节炎.本文研究痹症停合剂对大鼠佐剂性关节炎的防治,治疗作用,并观察其对大鼠腹腔巨噬细胞产生IL-1,TNF-a的影响,用以探讨其作用机制.为临床应用提供药理学依据.     

Immune stimulatory effects of Loranthi ramulus on macrophages through the increase of NO and TNF-α

The activation of macrophages by microorganisms plays an important role in host defense and immunopathology. Loranthi ramulus (LR) is commonly used as a traditional drug and health food in Korea. Here, we investigated the regulatory effects of LR on macrophage-mediated immune responses. Treatment of macrophages with LR resulted in the enhanced cell-surface expression of CD80, CD86 and major histocompatibility complex (MHC) class II, as well as the enhanced production of nitric oxide (NO) and tumor necrosis factor (TNF)-α, and also iNOS and TNF-α mRNA expression. These alterations of LR-treated cells were associated with the activation of NF-κB and mitogen-activated protein kinases (MAPKs). LR increased the phosphorylation of MAPKs (JNK, ERK1/2, p38 MAPK) and the activation of NF-κB in Raw 264.7 cells. These results suggest that LR has increased NO and TNF-α production through phosphorylation of all three MAPKs following IκBα degradation and NF-κB activation. In conclusion, our results demonstrate that LR can effectively promote the activation of macrophages, suggesting that LR may possess the potential to regulate immune responses.     

Does choice of antiretroviral drugs matter for inflammation?

Introduction: The massive implementation of combination antiretroviral therapy (cART) has forever changed the landscape of HIV infection. This unprecedented success has turned HIV infection into a manageable chronic disease. The increased survival of people living with HIV is, however, shadowed by a high burden of aging-related comorbidities. The pathogenic basis underlying this excess of co-morbid conditions is most likely a persistent inflammatory and immune activation state, despite an optimal control of HIV replication, which in turn has largely been attributed to bacterial or bacterial products translocation from the gut.

Area covered: This review is focused on the relationship between cART and the chronic inflammatory and immune activation status in otherwise virologically well-controlled people living with HIV (PLWH). Particular focus will be placed on the differences, if any, between distinct cART modalities, with emphasis on less-drug cART regimens, and especially on dual therapies.

Expert opinion: Research to address the increased inflammatory and immune activation status of cART-treated, HIV-infected patients, should focus on adjuvant means of therapy, rather than on the cART regime itself. With current antiretrovirals, no difference between dual and triple regimens has been demonstrated, provided that virological and immunological outcomes be non-inferior.     

冠心病与牙周病患者IL-6、TNF-a、PGE2水平相关性研究

目的：探讨冠心病与牙周病患者牙龈沟液中IL-6、TNF-a、PGE2水平变化的相关性。方法：临床选择60~74岁的120例患者,分为3组,分别应用放射免疫法检测龈沟液中IL-6、TNF-a、PGE2含量。结果：与健康对照组相比,冠心病伴牙周病组及单纯牙周病组中,龈沟液中IL-6、TNF-a、PGE2含量均明显升高（P〈0.05）;其中冠心病伴牙周病组龈沟液中IL-6、TNF-a、PGE2含量高于单纯牙周病组（P〈0.05）。结论：冠心病与牙周炎存在一定的相关性,伴有冠心病的牙周炎患者龈沟液内IL-6、TNF-a、PGE2水平明显升高。     

醒脑静注射液辅助治疗化脓性脑膜炎患儿观察

目的：观察醒脑静注射液联合头孢曲松治疗化脓性脑膜炎患儿的疗效。方法：100例化脓性脑膜炎患儿随机分为对照组50例和观察组50例,对照组予常规治疗;观察组在对照组基础上加用醒脑静注射液0.4-0.6ml·kg-1·d-1。两组疗程均为3 d。比较两组体温正常、外周血及脑脊液WBC计数正常时间与住院时间,以及两组患儿治疗前后血及脑脊液中肿瘤坏死因子-α（TNF-α）、C反应蛋白（CRP）水平变化,评价两组临床疗效,比较并发症及随访6个月后的后遗症发生情况。结果：与对照组相比,观察组体温正常时间和脑脊液WBC计数正常时间均明显缩短（P〈0.05）,血清及脑脊液TNF-α含量下降更显著（P〈0.05）,观察组临床总有效率明显高于对照组（P〈0.05）。其余指标两组比较差异无统计学意义（P〉0.05）。结论：醒脑静注射液辅助治疗化脓性脑膜炎能有效改善患儿症状,纠正炎症因子异常,临床疗效高,值得临床推广。     

氧化／抗氧化失衡在COPD稳定期慢性肺损伤的机制研究

目的探讨稳定期不同级别COPD患者血清中氧化／抗氧化因子及细胞炎症因子水平与肺功能主要指标的关系。方法选取COPD稳定期患者80例,检测各患者肺功能与血清中反应性氧核素（ROS）、超氧化物歧化酶（SOD）及细胞炎症因子水平,观测各组的差异并分析其相关性。结果FEV1、FVC、FEV1／FVC、SOD、ROS、TNF．d、IL-8、GM—CSF水平在不同组别中存在差异（P〈0．05）,ROS、TNF-a、IL-8、GM—CSF水平与FEV1,FVC、FEV1／FVC存在着明显的负相关（P〈0．05）,SOD与其存在明显正相关（P〈0．05）。结论COPD细胞炎症因子的持续升高及氧化加重可能是引起稳定期COPD肺组织慢性损伤、功能降低的重要机制。     

Anti-inflammatory effects of ethyl acetate fraction from Melilotus suaveolens Ledeb on LPS-stimulated RAW 264.7 cells

Aim of the study

This paper aimed to elucidate the anti-inflammatory effects of EtOAc fraction prepared from Melilotus suaveolens Ledeb ethanol extract with a cellular model of LPS-stimulated RAW 264.7 cell.

Materials and methods

Some key pro-inflammatory cytokines and mediators including IL-1β, IL-6, NO, iNOS, COX-2 and TNF-α, two important anti-inflammatory cytokines and mediators IL-10 and HO-1, I-κB and NF-κB were studied by sandwich ELISA, real-time PCR, western blot analysis and immunocytochemistry. At last a HPLC fingerprint was taken to evaluate the fraction.

Results

The EtOAc fraction could significantly inhibit the production of IL-1β, IL-6, NO, TNF-α, COX-2 in LPS-stimulated cell than that of single LPS-stimulated cell (p < 0.01 or p < 0.05), and the extract could increase the production of IL-10 and HO-1 than that of single LPS intervention cell (p < 0.01 or p < 0.05). Meanwhile, the extract also could inhibit the production of NF-κB compared to single LPS-stimulated cell. All the results showed that the extract had a good anti-inflammatory effect on LPS-stimulated RAW264.7 cell.

Conclusions

Taken together, the anti-inflammatory actions of M. suaveolens Ledeb EtOAc fraction might be due to the down-regulation of IL-1β, IL-6, NO, TNF-α and COX-2 via the suppression of NF-κB activation, and another pathway was up regulating the production of IL-10 and HO-1. Meanwhile, the EtOAc fraction might be further studied to isolate the active anti-inflammatory ingredients besides coumarin.     

阿魏酸钠对佐剂关节炎大鼠血清中瘦素和肿瘤坏死因子-a表达水平的影响

类风湿关节炎(RA)是一种以侵蚀性关节炎为主要表现的全身性自身免疫病,以女性多发,以双手和腕关节等小关节受累为主的对称性、持续性多关节炎。病理表现为关节滑膜的慢性炎症、血管翳的形成,并出现关节软骨和骨破坏,最终可导致关节畸形和功能丧失。阿魏酸钠(Sodium Ferulate,SF)是我国自行研发的一类新的非肽类内皮素受体拮抗剂,普遍存在于当归、川芎、蜂胶、酸枣仁等中药中,可拮抗内皮