绞股蓝多甙对家兔脂质代谢的影响

绞股蓝多甙１００ｍｇ／ｋｇ·ｄ－１×４ｗｋ灌胃给药，观察结果表明该物具有降低实验性高脂血症家兔的血脂水平及血清过氧化脂质、心肌褐脂质含量的作用。     

Central effects of systemic lidocaine mediated by glycine spinal receptors: an iontophoretic study in the rat spinal cord

The objective of this investigation was to demonstrate the possible interactions of systemic lidocaine (lido) with inhibitory receptors in the spinal cord. In the lumbar dorsal horn of anesthetized and curarized rats, 60 physiologically identified, wide dynamic range (WDR) neurons, were recorded extracellularly. Glutamate, glycine and its selective antagonist, strychnine, were iontophoretically applied onto the neurons either singularly or concurrently. The effects of systemic lido on the drug-induced frequency changes and the interaction with the glycine receptors, using strychnine as a probe, were studied. It was consistently found that (i) lido (3–4 mg/kg) inhibited the excitatory responses to iontophoretic glutamate, (ii) this inhibition was significantly antagonized by concurrent iontophoretic strychnine, (iii) iontophoretic glycine induced comparable glutamate inhibition that was reversed by strychnine. In contrast, no effect on glutamate-induced excitations was observed when lido was applied by micropressure or a different local anesthetic was systemically administered. The results suggest that central inhibitory effects of lido could by mediated by spinal strychnine-sensitive glycine receptors, activated by lido itself or possibly by its glycine residue-bearing metabolites.     

表面活性剂在硝酸士的宁注射液酸性染料比色法中的应用

表面活性剂在光度分析中已经有了广泛的应用,不论是在水溶液中测定的胶束增溶光度法还是在有机溶剂中测定的胶束萃取光度法中,表面活性剂的加入都具有明显的增溶、增敏和增稳作用,它能直接影响显色反应的速度、平衡位置以及配合物的构型,能改变溶剂的     

Pretreatment with aldosterone or corticosterone blocks the memory-enhancing effects of nimodipine,captopril, CGP 37 849, and strychnine in mice

Oral pretreatment with aldosterone or corticosterone blocked the memory-enhancing effects of the calcium antagonist nimodipine, the ACE inhibitor captopril, the NMDA blocker CGP 37 849, and the glycine antagonist strychnine in a passive-avoidance test in mice. The memory-disturbing effects of phenobarbitone, diazepam, CGP 37 849 and scopolamine were not influenced by the hormonal pretreatment. These findings could indicate the involvement of a steroid-sensitive mechanism in drug-induced improvement of memory. In the light of clinical observations showing elevated cortisol levels in Alzheimer patients, the results might also explain why only a limited number of these patients respond to therapy with memory enhancers.     

Effects of glycine and GABA,and blocking actions of strychnine and picrotoxin in the hypoglossus nucleus

Summary Neutral ω-amino acids were applied iontophoretically in the hypoglossus nucleus. Intracellular recordings revealed inhibitory actions involving hyperpolarization and conductance increase of the membrane. The antidromic field potential was reduced most effectively by glycine, as judged by the comparison of iontophoretic currents. Picrotoxin, ejected electrophoretically, clearly interfered with the action of GABA, glycine effects being reduced only with rather high currents. Strychnine had very specific blocking ability against glycine actions. Supported by the Deutsche Forschungsgemeinschaft (Br 242/7).     

Analytical studies on illicit heroin

The recovery of heroin in fumes was investigated. In the Netherlands the common mode of heroin smoking is the chasing the dragon procedure: heroin is heated on an aluminium foil by a lighter and the fumes are inhaled. The efficiency of the volatilization of heroin using this procedure was studied under laboratory conditions using thin layer chromatography, gas chromatography and high pressure liquid chromatography. A considerable influence of the form (salt or base) of the heroin was found as well as strong influences of other substances that may be present in illicit heroin samples as diluents. The danger of the inhalation of fumes containing unknown pyrolysis products is mentioned and a hypothesis is given for the phenomenon of heroin-leucoencephalopathy that was observed in heroin smokers in Amsterdam in 1981. The types of heroin encountered in the Netherlands are discussed with regard to their suitability for smoking.     

An in vitro functionally mature mouse spinal cord preparation for the study of spinal motor networks

An in vitro isolated whole spinal cord preparation has been developed in ‘motor functionally mature' mice; that is mice of developmental maturity sufficient to weight-bear and walk. In balb/c mice this stage occurs at around postnatal day 10 (P10). Administration of strychnine elicited synchronous activity bilaterally in lumbar ventral roots. Rhythmic alternating locomotor-like activity could be produced by application of a combination of serotonin (5-HT), N-methyl- -aspartate (NMDA), and dopamine in animals up to P12. Using a live cell–dead cell assay, it is demonstrated that there are primarily viable cells throughout the lumbar spinal cord. The viability of descending pathways was demonstrated with stimulation of the mid-thoracic white matter tracts. In addition, polysynaptic segmental reflexes could be elicited. Although usually absent in whole cord preparations, monosynaptic reflexes could invariably be elicited following longitudinal midline hemisection, leading to the possible explanation that there might be an active crossed pathway producing presynaptic inhibition of primary afferent terminals. The data demonstrate that this functionally mature spinal cord preparation can be used for the study of spinal cord physiology including locomotion.     

Effects of heroin,alone or in combination with other drugs,on the locomotor activity in two inbred strains of mice

The locomotor activity of C57B1/6J and DBA/2J mice was studied, under the influences of heroin, amphetamine, strychnine, or ethanol, and of combinations of the opiate with each one of the other drugs. Heroin treatment was followed by the typical running fit in the C57 mice, while the DBA strain was unaffected. Amphetamine enhanced the activity in the C57 strain only. The combination of heroin with amphetamine or ethanol increased the locomotor activity only in the DBA strain, while heroin + strychnine exerted a clear stimulating effect on the activity of the C57 mice. The strychnine + heroin mixture was more toxic than heroin alone when the lethal doses (LD50) were determined in the 2 strains.     

高效液相色谱法测定颈腰康胶囊中士的宁的含量

目的:探讨颈腰康胶囊中士的宁的含量测定方法。方法:采用高效液相色谱法。色谱柱:Lun silsca柱(4.6mm×250mm,5μm);流动 相:正己烷-二氯甲烷-甲醇-浓氨试液(42.5:42.5:5.4:0.35);检测波长:254nm。结果:士的宁的线性范围为0.1355～0.813μg (r=0.9998,n=5),平均加样回收率为100.6％,RSD=2.05％。结论:高效液相色谱法简便、灵敏、准确,可用于颈腰康胶囊的质量控制。     

薄层扫描法测定通痹灵片中士的宁的含量

目的：建立通痹灵片中士的宁的含量测定方法。方法：测定波长λ5=254nm，参比波长λR=325nm；展开剂为甲苯-丙酮-乙醇-浓氨试液(16：12：1：4)的上层液。结果：士的宁的平均回收率为97．53％；RSD为1．21％。结论：该方法简便、准确，可对产品质量进行控制。