前置胎盘孕妇恐惧心理观察及护理

目的观察前置胎盘孕妇的恐惧心理反应，做好前置胎盘孕妇的心理护理。方法由孕妇自行填写CES-D和SA2量表，进行统计学分析。结果70．2％的前置胎盘孕妇具有恐惧心理。结论在妊娠过程应提高孕妇认知水平，加强心理护理，有助于她们顺利渡过妊娠期。     

胎盘早剥的超声诊断

目的:探讨超声对胎盘早剥的诊断价值。方法:对过去16年间我院收治的19例经手术或阴道分娩证实的胎盘早剥患者的声像图与产后结果对照。结果:B超对Ⅰ度胎盘早剥(剥离面积<1/3)的诊断符合率为37.50%,对Ⅱ度(剥离面积1/3左右)、Ⅲ度胎盘剥离(剥离面积>1/2)的诊断符合率为88.89%。结论:超声对中重型胎盘早剥的诊断符合率较轻型患者为高。     

PLACENTAL CALCIUM PUMP: CLINICAL-BASED EVIDENCE

Placenta can be considered as a pump of calcium necessary for the normal development of the fetus. We believe that the location of this pump is in the placental basement membrane. The calcification of this membrane has been described only in cases of in utero fetal death. In this study we describe for the first time a case of placental calcification in a living fetus. The fetus of a normal 21-year-old pregnant woman showed heart abnormalities but the genetic analysis showed a normal male karyotype. The histology of the placenta demonstrated multiple intravillous linear and granular calcific incrustations The hemtoxylin/eosin stain of the sections revealed basement membrane calcific incrustations and intravillous calcium deposits. We postulate that the fetal circulation in the villi was impaired and the calcium that reached the villi from the mother was deposited at this level.     

人胎盘微绒毛膜的制备及其转铁蛋白受体研究

作者分别用差速离心法及蔗糖梯度离心法制备胎盘微绒毛膜(PMM),用受体放射分析法研究了PMM的转铁蛋白受体(TfB),结果表明:两种方法制备的PMM均可用于受体分析,但差速离心法更为简便。测定60例产妇PMM的TfR,其数目为3.53±1.98×10~(12)个位点/mg膜蛋白,最大结合容量为6.33±4.21×10~(-12)mol/mg膜蛋白,Kd值为4.95±3.39×10~(-9)mol/L。受体结合反应体系最适实验条件为,膜蛋白浓度每管50μg,标记物浓度每管50 000cpm,孵育30分钟,B/F分离的聚乙二醇浓度为12％(W/V)。对TfR的特性研究表明:TfR与转铁蛋白的结合具有高度亲和力、高度特异性及可饱和性。     

人胎盘CD133+细胞具有高增殖潜能集落形成细胞特性

目的 通过对人胎盘CD133⁺细胞群中高增殖潜能集落形成细胞(HPP-CFC)检测与生物学特性的分析，证明人胎盘存在早期造血干/祖细胞（HSPC）。 方法 采用机械法制备人胎盘组织（PT）单细胞悬液，用Histopaque-1007分离出单个核细胞(MNC)，经磁式分选（MACS）富集CD133⁺细胞，培养28 d后观察HPP-CFC集落形成能力，用流式细胞仪（FCM）对分选的细胞组份和HPP-CFC进行表型分析，实验全程用脐带血（UCB）作平行比较分析。 结果 培养28 d后，PT-CD133⁺与UCB-CD133⁺细胞组份分别扩增了266和362倍，前者低于后者（P<0.01）；PT-CD133⁺与UCB-CD133⁺细胞中HPP-CFC分别为(32.4±11.2)/5×10³、(17. 7±5.7)/5×10³，前者形成的HPP-CFC数量明显高于后者（P<0.01）；PT-CD133⁺、UCB-CD133⁺细胞培养至28 d时，除UCB-CD133⁺组的CD133⁺CD34^-亚群比例无明显改变外，CD133⁺CD34⁺、CD133^-CD34⁺和CD133⁺CD34^-（PT-CD133⁺组）亚型均比培养前减少。 结论 人胎盘组织CD133⁺细胞中存在HPP-CFC，说明胎盘CD133⁺细胞群中存在早期HSPC。     

水通道蛋白3在人胎盘和胎膜中的表达及分布

目的 研究正常妊娠晚期人胎盘和胎膜水通道蛋白3(AQP3)的表达及分布.方法 收集5例正常足月妊娠剖宫分娩的胎盘和胎膜样本,用RT-PCR测定AQP3 mRNA在胎盘和胎膜组织中的表达;用Western印迹和免疫组织化学方法检测AQP3蛋白质水平在胎盘和胎膜的表达.结果 RT-PCR显示AQP3 mRNA在胎盘和胎膜组织均有表达.Western印迹结果显示胎盘组织在29 000左右有一特异性条带.免疫组织化学结果显示AQP3表达于合体滋养细胞,而在羊膜上皮细胞未见表达.结论 AQP3在胎盘母儿液体平衡中可能发挥重要作用.     

Subperitoneal placenta accreta succenturiate in the case of a successful near-term extrauterine abdominal pregnancy

Placenta from an extrauterine abdominal pregnancy was examined after a 37-week healthy infant gestation. The placenta, with its fetal surface down and maternal surface up, protruded from the pelvic area to peritoneal cavity in the wall of the amniotic sac containing fetus. The placenta was implanted under the thin subperitoneal layer of maternal tissue completely covered by peritoneal serosa and was formed by several small lobes connected by intramembranous placental vessels. Insertion of the trivascular umbilical cord was velamentous. Partially remodeled arteries infiltrated by intermediate trophoblast and frequent veins directly communicating with the placental intervillous space were identified in the subperitoneal maternal tissue. The term "placenta accreta" is appropriate in this case because villi in the basal plate implanted directly in the maternal subserosal connective tissue without intervening decidua.     

Localization of the Antigen and Antibodies in the Placenta of Immunized Sheep

ABSTRACT: Placentomata of sheep immunized with human serum albumin (HSA) were examined. Both HSA and immunoglobulins were found in the maternal part and maternofetal border of the placenta using FITC labelled antisera on paraffin sections. Radiolabelled HSA was also detected in the fetal blood. The ultrastructure of placentomata revealed immunopathological process.     

妊高征患者胎盘血循环中血管舒、缩物质水平的改变及意义

目的　通过测定胎盘部位子宫静脉血中一氧化氮 (nitricoxide,NO)及内皮素 (endothelin ,ET)的浓度 ,并与外周血中同类物质的对比 ,了解妊高征时胎盘血管的病变程度及其与外周血中血管活性物质浓度改变的关系。方法　分别于剖宫产手术前及手术中抽取胎盘部位子宫静脉血和外周静脉血 ,应用硝酸根还原酶与Griess反应相结合的方法测定NO ;应用放射免疫分析法测定ET。结果　妊高征组胎盘部位子宫静脉血中NO2 -/NO3 -为 (70 2 6± 12 6 0 ) μmol/l,外周血清NO-2 /NO-3 为 (6 5 5 2± 14 88) μmol/l,二者之间无显著差异。妊高征组外周血浆ET水平为 (5 3 72± 15 2 8)ng/L ,胎盘部位子宫静脉血浆ET水平为 (5 2 80± 14 19)ng/L ,两者之间无显著性差异 (P >0 .0 1)。与正常晚孕组比较 ,妊高征组血中ET、NO-2 /NO-3 水平均显著增高 (P <0 .0 1)。结论　妊高征患者的子宫、胎盘循环系统血管舒、缩物质平衡失调 ,血管内皮系统的功能亦遭到破坏 ;妊高征患者胎盘血循环ET、NO水平升高 ,但与外周血中ET、NO水平的升高无关。