Antiulcer activity of Tinospora cordifolia Miers and Centella asiatica linn extracts

The ethanol extracts of the roots of T. cordifolia Miers and C. asiatica Linn were observed to induce a marked protective action against an 8 h restraint stress induced ulcerization, the activity being comparable to that of diazepam.     

余甘子功能食品的开发及其类SOD活力测定

余甘子是一种具有较高的食用和药用价值的野生植物资源，风味独特，含有丰富的类SOD活性物质和其他多种具有生物活性的物质，具有清除自由基、抗肿瘤、抗衰老等保健作用。作者提出了余甘予保健口服液的生产工艺流程及配方，并采用改良的Marklund方法测定了类SOD的活力，结果较为满意。     

高效液相色谱法测定叶下珠中没食子酸的含量

以高效液相色谱法测定叶下珠中没食子酸的含量。采用YWG C18( 10 μm)色谱柱 ,甲醇—水—磷酸 ( 4∶ 96∶ 0 0 5)为流动相 ,测得叶下珠中没食子酸的含量为 0 114% ,平均回收率为 10 3.1%。测定方法快速、灵敏 ,具有实用价值     

HPLC法测定马先蒿属三种药用植物中桃叶珊瑚苷的含量

目的 建立马先蒿属3种植物中桃叶珊瑚苷的测定方法。方法 药材用乙醚脱脂后，甲醇提取，Varian C18柱；流动相：甲醇-水(15：85)；检测波长：204nm；流速：0．5mL／min。结果 桃叶珊瑚苷在太白参、扭盔马先蒿、短茎马先蒿中的含量分别为0．90％，1．94％，0．95％；回收率分别为102．3％，98．2％，97．6％。结论 马先蒿属植物具有较好的药用开发前景，并为其质量评价提供参考。     

真菌诱导子对胡桐悬浮培养细胞产生红厚壳素的影响

目的探讨真菌诱导子对胡桐悬浮培养细胞产生红厚壳素的影响。方法通过对胡桐叶斑病病菌的分离培养,制成真菌诱导子补加到胡桐细胞悬浮培养基中,考察不同浓度、不同加入时间对胡桐细胞生物量及红厚壳素产量的影响。结果S-I菌株诱导胡桐CR2细胞合成红厚壳素的最佳浓度为60 mg GE·L^-1,诱导子在细胞生长静止期初期(即培养d 18)加入对红厚壳素产量影响最大,可使产量提高27%,并促进了红厚壳素向胞外分泌。结论 壳多孢菌的添加能有效提高胡桐细胞悬浮培养体系中红厚壳素的产量。     

福建叶下珠抗小鼠免疫性肝损伤的实验研究

目的评价福建叶下珠对小鼠免疫性肝损伤的保护作用.方法48只NIH小鼠随机分为叶下珠高、低剂量组、联苯双酯组、正常对照组及模型组.于造模之日起,叶下珠高、低剂量组分别以20 g/kg、10 g/kg叶下珠药液灌胃,联苯双酯组以0.15 g/kg联苯双酯药液灌胃,正常对照组及模型组每天给予等量生理盐水.每天灌胃1次,连用12 d.检测血清谷丙转氨酶(ALT)和肝、脾脏器指数,并做肝脏病理学检查.结果联苯双酯组、叶下珠高、低剂量组小鼠血清ALT、肝指数、脾指数值较模型组有不同程度的改善(P＜0.05,P＜0.01).炎症坏死程度:5组间比较,差异有非常显著性意义(P＜0.005);模型组与正常组、联苯双酯组、福建叶下珠高、低剂量组与模型组间两两比较,差异有显著性意义(P值均＜0.05);联苯双酯组与福建叶下珠大、小剂量组间比较,差异无显著性意义(P＞0.05).结论福建叶下珠能显著降低血清转氨酶活力和肝脾脏器指数,对肝细胞坏死有一定的改善作用,具有较好的抗小鼠免疫性肝损伤作用.     

中国宽叶缬草药用功效研究概况

从宽叶缬草的镇静活性、抗抑郁活性、调节血脂及抗脂质过氧化作用、对肾脏的保护作用、以及对胆囊结石和心、脑血管系统疾病的治疗作用等七个方面综述了近三年来国内对宽叶缬草药用功效的研究进展。     

RP-HPLC测定黄珠子草中短叶苏木酚的含量

目的:建立高效液相色谱法测定黄珠子草中短叶苏木酚的含量。方法:以 Thermo Hypersil-Keystone ODS C_(18)为分析柱,甲醇-1%醋酸(36:64)为流动相,流速1.0mL·min~(-1),检测波长278nm,采用外标法定量测定。结果:短叶苏木酚在10～100μg·mL~(-1)范围内线性关系良好(r=0.999 7);其平均回收率为98.77%,RSD 为0.98%(n=6)。结论:该方法简便可靠,快速,重现性好,可用于黄珠子草中短叶苏木酚的含量测定。     

Potato Disc Bioassay for Some Jordanian Medicinal Plants

Fifteen different plants representing different species, genera and families were used to test the effect of their aqueous extracts on tumor inhibition with a rapid, inexpensive, safe, animal sparing technique, and statistically reliable prescreen for in vivo murine leukemia antitumor activity, which is known as potato disc technique. The inoculum used contains the extract with the bacterium (Agrobacterium tumefaciens) that was known to cause crown gall tumor to different plant species especially dicotyledons and some gymnosperms. After 12 to 21 days of incubation the tumors appeared and were counted, and the maximum inhibition was then calculated. Thirteen species out of fifteen showed a good antitumor effect, and three of them showed a maximum inhibition of 80%. More experiments are necessary to determine the exact mechanism of action.     

Antioxidative Potential of Duranta Repens (Linn.) Fruits Against H2O2 Induced Cell Death In Vitro

The effects of Duranta repens fruits were investigated on H₂O₂ induced oxidative cell death to evaluate its antioxidative potential in vitro. HEK293T cells were treated with different concentrations [0–1000 µg/ ml] of ethanol extract (E-Ex) and methanol extract (M-Ex) of D. repens for 24h, and then treated with 100 µM H₂O₂ for 24h. Cell viability, antioxidant parameters of cells, and antioxidant constituents of the extracts were determined. Treatment with limited dose of E-Ex or M-Ex increased the survival rate of H₂O₂-treated HEK293T cells, however the extra-high dose showed growth inhibitory effect. Treatment with E-Ex or M-Ex protected cellular lipid per-oxidation. In vitro analyses showed the 2,2-diphenyl-1-picrylhydrazyl and H₂O₂ scavenging activities as well as reducing potential of the extracts. We report here that the limited dose of E-Ex and M-Ex possess antioxidative potential, which can protect H₂O₂-induced oxidative cell damage.