拟盘多毛孢菌中新倍半萜的分离与结构解析(英文)

目的:研究一株拟盘多毛孢菌(Pestalotiopsis sp.)的化学成分。方法:采用硅胶(silica gel,Rp-18 silica gel)和凝胶(Sephadex LH-20)柱层析的方法分离和纯化化合物,其结构由核磁共振波谱(1H NMR,13C NMR,DEPT,1H-1H COSY,HSQC,HMBC和NOESY)和HR ESI-Q-TOFMS解析。结果:从拟盘多毛孢菌的静置发酵培养液中分离到一个倍半萜化合物(1)和一个已知化合物4-羟基苯乙醇。结论:化合物1为新化合物。     

DNA methyltransferase inhibitor dramatically alters the secondary metabolism of Pestalotiopsis microspora

5-Azacytidine, a DNA methyltransferase inhibitor, led to significant changes in the secondary metabolism of the plant endophytic fungus, Pestalotiopsis microspora. Analysis of the culture broth extract led to the isolation of a new compound, 4′-formamidophenyl-5-methoxybenzoate (1), along with seven known polyketides, 4-hydroxybenzoic acid (2), LL-P880α (3), 1′-hydroxy-4-methoxy-6-pentyl-2H-pyran-2-one (4), pestalotiollide B (5), pestalotiopyrone G (6), endocrocin (7) and 2′-hydroxy-6′-hydroxymethyl-4′-methylphenyl-2,6-dihydroxy-3-(2-isopentenyl)benzoate (8). HPLC profiles revealed that all compounds except for 4 belonged to the newly induced secondary metabolites. In addition, all compounds were proved to be devoid of significant antifungal activity in the bioassays.     

Endophytic fungi with antitumor activities: Their occurrence and anticancer compounds

Abstract

Plant endophytic fungi have been recognized as an important and novel resource of natural bioactive products, especially in anticancer application. This review mainly deals with the research progress on the production of anticancer compounds by endophytic fungi between 1990 and 2013. Anticancer activity is generally associated with the cytotoxicity of the compounds present in the endophytic fungi. All strains of endophytes producing antitumor chemicals were classified taxonomically and the genera of Pestalotiopsis and Aspergillus as well as the taxol producing endophytes were focused on. Classification of endophytic fungi producing antitumor compounds has received more attention from mycologists, and it can also lead to the discovery of novel compounds with antitumor activity due to phylogenetic relationships. In this review, the structures of the anticancer compounds isolated from the newly reported endophytes between 2010 and 2013 are discussed including strategies for the efficient production of the desired compounds. The purpose of this review is to provide new directions in endophytic fungi research including integrated information relating to its anticancer compounds.     

源于植物内生无花果拟盘多毛孢真菌的新环氧环己二醇衍生物(英文)

从一株植物内生无花果拟盘多毛孢真菌的放大发酵产物中获得了7个新结构的异戊二烯取代环氧环己二醇衍生物,分别命名为pestalofones F-H(1-3)和pestalodiols A-D(4-7),并应用MS、NMR等光谱技术鉴定了其结构。化合物1-3,6和7对HeLa和MCF-7肿瘤细胞株具有中等程度的细胞毒活性。     

一种西沙群岛软珊瑚来源真菌中新天然产物pestalalactone的化学与生物活性研究

目的 从一株中国南海西沙群岛软珊瑚来源的真菌Pestalotiopsis sp. (ZJ-2009-7-6) 中分离鉴定具有生物活性的3-苯基苯并呋喃酮化合物。方法 运用多种色谱分离手段和现代波谱分析方法分离和鉴定化合物,最终得到了一个3-苯基苯并呋喃酮类新天然产物pestalalactone (1),并研究了该化合物在不同温度、不同溶剂条件下的优势构象变化;此外,还对化合物的抗藤壶幼虫Balanua amphitrite附着和抗细菌活性进行了评价。结果 分离鉴定了1个新天然产物 pestalalactone (1),发现该化合物以两种不可拆分的阻转异构体的形式 (1a/1b) 并存;核磁共振氢谱研究后发现在氘代吡啶和氘代氯仿中以1a优势构象存在,在氘代甲醇、氘代丙酮和氘代二甲亚砜中的优势构象却是1b,而温度的变化 (25°C–80°C) 并不引起该化合物构象的变化;该化合物显示出强的抗藤壶幼虫B. amphitrite附着活性,其EC₅₀值为2.30 μg/mL,同时也显示出较强的抗细菌活性,特别是对金黄色葡萄球菌Staphylococcus aureus的最小抑制活性为0.45 μg/mL。结论 从一株西沙群岛软珊瑚来源真菌中发现了一个具有重要抗藤壶幼虫B. amphitrite附着和抗菌活性的新天然产物pestalalactone (1),表明西沙群岛来源的海洋微生物具有重要的药用研究价值和应用开发前景。     

Characterization of Pestalotiopsis sp. causing gray leaf spot in coconut (Cocos nucifera L.) in Bangladesh

Coconut (Cocos nucifera L.) is one of the most important fruit trees in Bangladesh. This tree is susceptible to various pathogens. Among them, a fungus was consistently isolated from gray leaf spot symptom in coconut. This study aimed to isolate, characterize, and find the management strategies of the causal fungus of gray leaf spot disease in coconut. Both morphological and molecular characters identified the pathogen as Pestalotiopsis sp. for the first time in Bangladesh. Artificial inoculation of this fungus showed symptoms similar to those previously observed in the field. Cross-inoculation test suggests that Pestalotiopsis sp. has a wide host range. The infection process of Pestalotiopsis sp. started at 2 h after inoculation (hai) with the formation of germ tube followed by the formation of infection hyphae, which penetrated directly into the host at 6 hai. Gray leaf spot symptom was developed at 120 hai. Numerous conidia developed from the acervuli at 168 hai. These conidia acted as the source of inocula for secondary infection. The optimum temperature for the growth of Pestalotiopsis sp. was 25°C, however, the growth of Pestalotiopsis sp. ceased at 15°C and 35°C. This pathogen was completely inhibited by Autostin 50 WDG (carbendazim) at 100 ppm. Trichoderma viride (Pb-7) was found as the potential biocontrol agent against Pestalotiopsis sp. These findings could contribute to describing the disease cycle and epidemiology of Pestalotiopsis sp. that would ultimately require to undertake effective control measures against this pathogen.     

Pestalotiopamide E,a new amide from the endophytic fungus Pestalotiopsis sp.

Chemical examination of the endophytic fungus Pestalotiopsis sp., isolated from the leaves of the Chinese mangrove Rhizophora mucronata, yielded a new amide called pestalotiopamide E (1). The structure of the new compound was unambiguously elucidated on the basis of extensive spectroscopic data analysis.     

Polyketides from Pestalotiopsis zonata and structure revision of pestalrones A and B

The structures of pestalrones A-B were revised via reinterpretation of the NMR data and a brief chemical transformation from the co-occurring polyketides, in our investigation on the secondary metabolites of Pestalotiopsis zonata, which also afforded a new α-pyrone derivative, pestazonatic acid, and four known analogs.     

环带拟盘多毛孢发酵液中一个新的联苯类化合物(英文)

目的:研究分离于海南红槟榔中环带拟盘多毛孢发酵液的化学成分。方法:利用硅胶柱层析和凝胶柱层析分离纯化得到4个化合物(1~4),借助光谱学手段鉴定其结构。测试了化合物1对大肠埃希氏菌、金黄色葡萄球菌、铜绿假单胞菌、肺炎克来伯氏菌、MRSA耐甲氧西林金黄色葡萄球菌、鲍曼不动杆菌和VRE耐万古霉素屎肠球菌的抑制活性。结果:分离鉴定了4个化合物包括1个新化合物(2'-乙酰基-4',4-二甲氧基联苯-2-醛基),该化合物对7种细菌株有较弱抑制活性,IC50分别为0.75,0.75,0.82,0.81,0.84,0.90和0.87μm·mL-1.结论:化合物1为新的联苯类化合物,对7种细菌株表现出比较弱的抑制活性。化合物2~4为首次从该种中分离得到。