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人参皂甙Rg1对大鼠局灶性脑缺血脑组织神经干细胞增殖的影响 总被引:4,自引:0,他引:4
目的 观察人参皂甙Rg1对大鼠局灶性脑缺血脑组织神经干细胞增殖的影响,探讨其在脑保护及抗衰老作用中的可能机制.方法 取成年雄性Wistar大鼠,用线栓法制作大鼠大脑中动脉闭塞(MCAO)模型,采用脉冲标记法,经腹腔注射5′-溴脱氧尿苷(BrdU)标记处于增殖状态的神经干细胞,用免疫组织化学单标及双标免疫荧光技术观察人参皂甙Rg1对局灶性脑缺血后BrdU和巢蛋白(nestin)的免疫活性及nestin/BrdU双标阳性细胞的影响,镜下观察脑内神经干细胞的分布,并计数行定量分析.结果 局灶性脑缺血后大鼠室管膜下区及海马齿状回有nestin、BrdU及nestin/BrdU双标免疫阳性细胞分布.应用人参皂甙Rg1后,上述各部位阳性细胞数明显增多,与缺血组之间有显著性差异(P<0.01).结论 人参皂甙Rg1具有促使神经干细胞增殖的能力,这可能是其神经保护及抗衰老作用的机制之一. 相似文献
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目的:建立测定驯服家犬血浆中血塞通分散片的主要成分人参皂苷Rg1的高效液相色谱方法。方法:血浆样品采用蛋白沉淀法,以纯化水-乙腈为流动相,经ZORBAX Edipse XDB-C8色谱柱分离,在203nm波长检测。结果:Rg1质量浓度在2.58~51.6mg·L^-1范围内线性关系良好,最低检测质量浓度为2.58mg·L^-1。低、中、高3个质量浓度的提取回收率大于85%。日内、日间相对标准偏差低于10%。结论:本方法准确、快速,可满足临床药动学研究的要求。 相似文献
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目的 制定长寿液质量标准。方法 采用了LC法对处方中的枸杞子、当归和川芎等3种药材追行定性鉴别;采用HPLC法对处方中人参的人参皂苷RgI追行了含量测定,色谱条件:依利特Hypersil C18色谱柱(4.6×150mm,5μm);流动相乙腈-0.1%磷酸溶液(19.8:70.2);流速1.0ml/min;检测波长为203nm。结果 人参皂苷Rgl在0.0298125—0.477mg/ml范围内线性关系良好,r=1.0000,平均回收率为101.50%,RSD为0.61%。结论 本法操作简便,能准确、可靠地追行定性、定量检测,可有效控制长寿液的质量。 相似文献
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目的建立大七散的质量标准。方法采用高效液相色谱法对大七厘散中的主要成分人参皂苷Rg1进行定量分析,λ=203nm;同时对制剂中其他主要药材大黄、冰片、当归尾进行薄层鉴别。结果能准确对大黄、冰片、当归尾进行定性鉴别;人参皂苷Rg1在0.5710-4.2840ug范围呈良好线性关系。结论本方法快速、简便、准确、重现性好。 相似文献
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Ai-wu SHI ;Xiao-bin WANG ;Feng-xiang LU ;Min-min ZHU ;Xiang-qing KONG ;Ke-jiang CAO 《中国药理学报》2009,(3):299-306
Aim: To investigate the effect of ginsenoside Rgl on the migration, adhesion, proliferation, and VEGF expression of endothelial progenitor cells (EPCs).
Methods: EPCs were isolated from human peripheral blood and incubated with different concentrations of ginsenoside Rgl (0.1, 0.5, 1.0, and 5.0 μmol/L) and vehicle controls. EPC migration was detected with a modified Boyden chamber assay. EPC adhesion was determined by counting adherent cells on fibronectin-coated culture dishes. EPC proliferation was analyzed with the 3.(4,5.dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In vitro vasculogenesis was assayed using an in vitro vasculogenesis detection kit. A VEGF-ELISA kit was used to measure the amount of VEGF protein in the cell culture medium.
Results: Ginsenoside Rgl promoted EPC adhesion, proliferation, migration and in vitro vasculogenesis in a dose- and timedependent manner. Cell cycle analysis showed that 5.0 μmol/L ofginsenoside Rgl significantly increased the EPC proliferative phase (S phase) and decreased the resting phase (G0/G1 phase). Ginsenoside Rgl increased vascular endothelial growth factor production. Conclusion: The results indicate that ginsenoside Rgl promotes proliferation, migration, adhesion and in vitro vasculogenesis. 相似文献
Methods: EPCs were isolated from human peripheral blood and incubated with different concentrations of ginsenoside Rgl (0.1, 0.5, 1.0, and 5.0 μmol/L) and vehicle controls. EPC migration was detected with a modified Boyden chamber assay. EPC adhesion was determined by counting adherent cells on fibronectin-coated culture dishes. EPC proliferation was analyzed with the 3.(4,5.dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In vitro vasculogenesis was assayed using an in vitro vasculogenesis detection kit. A VEGF-ELISA kit was used to measure the amount of VEGF protein in the cell culture medium.
Results: Ginsenoside Rgl promoted EPC adhesion, proliferation, migration and in vitro vasculogenesis in a dose- and timedependent manner. Cell cycle analysis showed that 5.0 μmol/L ofginsenoside Rgl significantly increased the EPC proliferative phase (S phase) and decreased the resting phase (G0/G1 phase). Ginsenoside Rgl increased vascular endothelial growth factor production. Conclusion: The results indicate that ginsenoside Rgl promotes proliferation, migration, adhesion and in vitro vasculogenesis. 相似文献
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人参皂苷Rgl抗肝纤维化的实验研究 总被引:3,自引:0,他引:3
[目的]观察三七总甙单体成分人参皂苷Rg1对实验性肝纤维化的作用.[方法]用50%CC14致大鼠肝纤维化模型,共35 d,同时给予三七总皂甙单体成分人参皂苷Rg1治疗,于实验第2、5周(实验结束)时检测肝功能、血清Ⅲ型前胶原(PCⅢ)、透明质酸(HA)、层黏连蛋白(LN),于实验结束时分离肝组织光镜观察其病理变化.[结果]人参皂苷Rg1及三七总皂甙能改善肝纤维化大鼠的肝功能,降低血清PC Ⅲ、HA、LN水平;人参皂苷Rg1中、高剂量组及三七总皂甙明显减轻肝组织胶原的沉积,改善肝纤维化程度.[结论]人参皂苷Rg1及三七总皂甙具有抗肝纤维化作用,人参皂苷Rg1是三七总甙抗肝纤维化有效成分之一. 相似文献
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目的:建立糖泰片的质量标准。方法:采用TLC法对糖泰片中人参、地黄和黄芪进行定性鉴别,HPLC法测定制剂中人参皂苷Rg1和人参皂苷Re的含量。结果:在薄层色谱中能检出人参、地黄和黄芪;人参皂苷Rg1在21.3~213μg/mL、人参皂苷Re在20.6~206μg/mL范围内线性良好,回归系数r分别为0.9998和0.9996。结论:建立的分析方法简便可行,专属性强,可用于糖泰片的质量控制。 相似文献
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