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1.
Several studies have demonstrated that a descending dopaminergic pathway innervates the dorsal and the intermediate gray matter of the spinal cord and have suggested that this pathway is involved in pain modulation and in the control of autonomie functions. Other studies have also demonstrated the presence of dopamine (DA) and DA metabolites as well as of DA receptors in the ventral cord. There is also evidence for the implication of DA in the control of motor functions at the spinal level. The occurrence of a dopaminergic innervation in the ventral horn has been, however, disputed until recently. But recent work has demonstrated that the motoneural cell groups in the ventral horn (lamina IX) are a target for descending dopaminergic fibers. In addition, the possibility that DA is a mediator of primary afferent fibers has also been postulated. Finally, the occurrence of dopaminergic cell bodies has been suggested in the spinal cord. This indicates that DA is probably implicated in a complex manner in spinal functions. In the present paper the possible involvement of DA in sensory and in motor functions at spinal level will be discussed in view of neurochemical observations made in polyarthritic rats, in which pain-related behavior and reduction of locomotor activity associated with a marked decrease in mobility, are observed. 相似文献
2.
Interactions between 5-hydroxytryptamine (5-HT) and substance P (SP) in the mouse spinal cord were investigated using the tail-flick test and the behavioral response evoked by intrathecal (i.th.) SP or i.th. 5-HT. I.th. injection of 5-HT (20 μg) or the 5-HT1 receptor agonists(+)-8-hydroxy-2-(di-n-propylamino)tetralin ((+)-8-OH-DPAT) (20 μg) or 5-methoxy-3(1,2,3,6-tetrahydropyridine-4-yl)-1H-indole (RU 24969) (20 μg) markedly inhibited the tail-flick reflex. The effect of these compounds was reduced when SP (5 μg) was given i.th. 55 min, or 55 and 45 min before the agonists. The tail-flick latencies recorded 5 min before injection of a 5-HT agonist were similar in animals treated with SP or vehicle. The changes in the tail-flick test were not due to changes in tail skin temperature since only minimal differences in the skin temperature were recorded between the groups injected with SP or vehicle. I.th. injection of SP (10 ng) or 5-HT (2 μg) produced a similar behavioral response consisting of biting, licking and scratching of the caudal part of the body, indicative of nociceptive stimulation. The responses both to i.th. SP and 5-HT were reduced after i.th. application of SP receptor antagonist [d-Arg1,d-Trp7,9,Leu11]-SP (Spantide) (5 μg), as well as 5 min after i.th. injection of the 5-HT receptor antagonist metergoline (4 μg). The data may indicate functional interactions between SP and 5-HT in the mouse spinal cord, which may take place in neurons involved in the processing of nociception. 相似文献
3.
A. D. Craig Jr J. O. Dostrovsky 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1991,85(2):470-474
Summary The technique of antidromic mapping with a roving array of electrodes was used to demonstrate that lamina I trigeminothalamic cells responsive specifically to skin temperature project to the n. submedius (Sm) in the medial thalamus of the cat. This finding indicates that Sm receives thermoreceptive in addition to nociceptive information. 相似文献
4.
Anatomical studies have revealed the existence of an ascending pathway originating in the spinal cord and medullary dorsal horn, relaying in nucleus submedius (Sm) in medial thalamus and terminating in ventrolateral orbital cortex. It has been suggested that this pathway may be involved in the transmission of nociceptive information. In the present study extracellular recordings were obtained from neurons in Sm of anesthetized arthritic and normal rats. Mechanical and thermal stimuli were delivered to various regions of the body to determine the types of somatic stimuli which could activate Sm neurons. Over 40% of the 146 neurons studied responded to somatic stimuli. In the normal rats only high intensity mechanical and thermal stimuli were effective in inducing responses. In the arthritic rats lower intensity mechanical stimuli, joint movements and high intensity thermal stimuli were effective. Such stimuli produce nociceptive reactions in the freely moving arthritic rat. Almost all the responses were excitatory and generally lasted the entire duration of the 15-s stimuli employed. In some cases after-discharges were present. The receptive fields of the neurons were in almost all cases large and bilateral. These findings support the hypothesis that Sm may be involved in mediating the affective-motivational aspects of pain. 相似文献
5.
Discovering genes: the use of microarrays and laser capture microdissection in pain research 总被引:2,自引:0,他引:2
Siobhan C. Reilly Andrew R. Cossins John P. Quinn Lynne U. Sneddon 《Brain Research Reviews》2004,46(2):225
The DNA microarray is a powerful, high throughput technique for assessing gene expression on a system-wide genomic scale. It has great potential in pain research for determining the network of gene regulation in different pain conditions, and also for producing detailed gene expression maps in anatomical areas that process nociceptive stimuli. However, for the potential of this high throughput technology to be realised in pain research, microarrays need to be combined with other technologies. Laser capture microdissection is capable of isolating small populations of homogenous cells, allowing distinct areas involved in nociceptive processing to be examined. In combination with sophisticated PCR-based amplification protocols this technique provides sufficient amounts of messenger RNA (mRNA) for application to microarrays. Aside from the technological issues, a difficult task in any microarray study is the analysis of the resulting enormous data set to reveal the key genes, whose regulation is central to the phenotypic changes observed. For this to be achieved, the methods of data analysis, pattern searching and feature recognition, and bioinformatics have to be properly deployed all within the context of an appropriate statistical design. These issues are especially relevant to pain research where interindividual and interpopulation variation is likely to be high, and where polymorphisms can greatly affect nociceptive sensitivity and susceptibility to pain conditions. Methods for assessing the function of new candidate genes identified in microarray screening experiments are also discussed. 相似文献
6.
通过动物痛行为反应(缩足反射)确定局部和鞘内应用川芎嗪(TMP)对ATP等P2X受体激动剂所致大鼠足底急性伤害性行为反应的影响。P2X3受体拮抗剂TNP-ATP(0.3μmol/L)明显抑制P2X受体激动剂ATP(1μmol/L)或α,β-meATP(0.6μmol/L)引起的大鼠足底急性伤害性反应。大鼠足底局部应用TMP(0.1-10mmol/L)剂量依赖性地对ATP(1μmol/L)或α,β-meATP(0.6μmol/L)引起的伤害性反应具有抑制作用。鞘内应用TMP(50mmol/L)对ATP(1μmol/L)或α,β-meATP(0.6μmol/L)引起的伤害性反应具有抑制作用。结果表明,TMP可通过阻断P2X3受体介导的伤害性兴奋传入抑制P2X受体激动剂引起的大鼠足底急性伤害性反应。 相似文献
7.
Maxwell DJ Kerr R Rashid S Anderson E 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》2003,149(1):114-124
Serotonin 5-HT3 receptors are abundant in the superficial dorsal horn and are likely to have an involvement in processing of nociceptive
information. It has been shown previously that 5-HT3 receptors are present on primary afferent terminals and some dorsal horn cells. The primary aim of the present study was
to determine what classes of primary afferent possess 5-HT3A receptor subunits. We performed a series of double- and triple-labelling immunofluorescence experiments. Subunits were labelled
with an anti-peptide antibody and primary afferent axons were identified by the presence of calcitonin gene-related peptide
(CGRP) and binding of the lectin IB4. Quantitative confocal microscopic analysis revealed that approximately 10% of axons
displaying 5-HT3A immunoreactivity were also labelled for CGRP but that only 3% of these fibres bind IB4. We also investigated the relationship
between immunoreactivity for the subunit and descending serotoninergic systems, axons originating from inhibitory neurons
that contain glutamic acid decarboxylase, and axons of a subpopulation of excitatory neurons that contain neurotensin. None
of these types of axon was associated with immunoreactivity for receptor subunits. Ultrastructural studies confirmed that
punctate immunoreactive structures observed with the light microscope were axon terminals. These terminals invariably formed
asymmetric synaptic junctions with dendritic profiles and often contained a mixture of granular and agranular vesicles. Some
terminals formed glomerular-like arrangements. Immunoreactive cells were also examined and were found to contain intense patches
of reaction product within the cytoplasm. We conclude that the majority (about 87%) of dorsal horn axons that are immunoreactive
for 5-HT3A receptor subunits do not originate from the subtypes of primary afferent fibres that bind IB4 or contain CGRP. It is likely
that most of these axons have an excitatory action and they may originate from dorsal horn interneurons and/or fine myelinated
primary afferent fibres.
Electronic Publication 相似文献
8.
U. Heinemann H. G. Schaible R. F. Schmidt 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1994,79(2):283-292
Summary In 20 cats anaesthetized with alpha-chloralose and spinalized at the thoracolumbar junction we investigated the role of stimulation induced accumulation of extracellular potassium in the spinal cord in the processing of nociceptive discharges from the knee joint. For that we electrically stimulated the posterior articular nerve of the knee. We further performed innocuous and noxious stimulation of the knee and of other parts of the leg and studied the effect of an acute inflammation of the knee on [K+]0 in the spinal cord. Innocuous stimulation of the skin (brushing or touching) and innocuous movements in the knee joint all induced rises in [K+]0 which were maximal at recording depths of 1500 to 2200 m below the surface of the cord dorsum. Peak increases were 0.4 mM for touching the leg and 1.7 mM during rhythmic flexion/ extension of the knee joint. Noxious stimulation of the skin, the paw, the tendon and noxious movements of the knee joint also produced rises in [K+]0, which were somewhat larger for the individual types of stimuli than those produced by innocuous intensities. Electrical stimulation of the posterior articular nerve induced rises in [K+]0 by up to 0.6 mM. Stimulus intensities sufficient to activate unmyelinated group IV fibers were only slightly effective in raising [K+]0 above the levels reached during stimulation of myelinated group II and III fibers. During development of an acute inflammation of the knee joint (induced by kaolin and carrageenan), increases in [K+]0 and associated field potentials became larger by about 25%. We assume that this reflects an increase in neuronal responses. In conclusion, changes in [K+]0 in the spinal cord are some-what larger during noxious stimulation than during innocuous stimulation. The absolute level reached depended more on the site and type of stimulation than on the actual stimulus intensity itself. Hence a critical role of spinal K+ accumulation for nociception is unlikely. 相似文献
9.
A. S. Zagami G. A. Lambert 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1990,81(3):552-566
Summary Extracellular recordings were made in the thalamus of cats anaesthetized with chloralose and urethane following electrical,
mechanical and chemical stimulation of the superior sagittal sinus or middle meningeal artery. Facial receptive fields were
looked for using electrical and mechanical stimuli. The locations of fifty-six cells were verified histologically. Twenty
six cells were located in the ventroposteromedial nucleus (VPM) and six in its ventral periphery (VPMvp). All units in VPM
had facial receptive fields, usually involving the first trigeminal division. Cells with nociceptive receptive fields or responding
to the craniovascular application of bradykinin were often found in the periphery or “shell” region of VPM. Other craniovascular
nociceptive cells were found in VPMvp, in the posterior group and in the intralaminar complex. This study shows that craniovascular
afferents in the cat project to several thalamic nuclei and implicate VPM especially in craniovascular nociception. 相似文献
10.
J. Siegel C. R. Morton J. Sandkühler H. -M. Xiao M. Zimmermann 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1986,62(2):363-372
Summary In cats anaesthetized with sodium pentobarbital and 70% N2O, single lumbar dorsal horn neurons were excited by controlled noxious radiant heating of glabrous hindpaw skin. The EEG was recorded from the pericruciate cortex and posterior lateral gyrus. Subcortical forebrain sites where electrical stimulation inhibited dorsal horn neuronal heat-evoked responses contralaterally were identified by mapping the caudate nucleus, internal capsule, septum, nucleus accumbens and basal forebrain regions. Inhibitory sites were mainly located in the ventral forebrain (ventral septum, diagonal band, basal forebrain). The caudate nucleus and internal capsule had a low incidence and effectiveness of inhibitory sites. In the basal forebrain, the incidence and effectiveness of inhibitory sites decreased from caudal to rostral regions. There was a rostral limit of inhibitory sites, both medially and laterally. The magnitude of inhibition increased with graded increases in brain stimulation intensity. The mean incremental increase in inhibition was greater for caudal than for rostral basal forebrain sites. Mean stimulus currents for threshold of inhibition and for inhibition to 50% of control heat responses were lower for caudal than for rostral sites. Responses of the dorsal horn neurons to increasing temperatures of noxious skin heating were monotonic linear functions over the temperature range studied (48–53° C). Stimulation in both rostral and caudal basal forebrain decreased the slope of this stimulus-response function, with a greater decrease for caudal sites. Cortical EEG synchronization was evoked by stimulation in the caudate nucleus and rostral basal forebrain. For both regions, most synchronogenic sites did not produce descending inhibition of dorsal horn neurons. The significance of these findings in relation to descending inhibition from other brain regions and stimulation-produced analgesia is discussed. 相似文献