应用近红外反射光谱技术测定油菜芽期耐旱性的研究

针对油菜芽期耐旱鉴定，提出了用近红外反射光谱技术（NIR法）预测油菜吸胀24 h电导率、PEG模拟干旱条件下的相对发芽率、相对鲜重和鲜重耐旱指数等4个芽期耐旱相关性状的方法。以采集的49份不同耐旱水平甘蓝型油菜近红外光谱数据为基础，采用偏最小二乘法和多元回归算法建立了最优定标模型，并获得较高的决定系数(0.71～0.86)和较低的标准误差(1～15.65)。验证集评估结果表明，NIR法与室内鉴定法测定油菜4个芽期耐旱相关性状无显著差异，且具有极显著的相关关系(决定系数0.72～0.89)。研究表明,近红外光谱技术用于油菜芽期耐旱性鉴定是可行的，可用于耐旱育种早代选择。     

Optical spectroscopic determination of human meniscus composition

This study investigates the correlation between the composition of human meniscus and its absorption spectrum in the visible (VIS) and near infrared (NIR) spectral range. Meniscus samples (n = 24) were obtained from nonarthritic knees of human cadavers with no history of joint diseases. Specimens (n = 72) were obtained from three distinct sections of the meniscus, namely; anterior, center, posterior. Absorption spectra were acquired from each specimen in the VIS and NIR spectral range (400–1,100 nm). Following spectroscopic probing, the specimens were subjected to biochemical analyses to determine the matrix composition, that is water, hydroxyproline, and uronic acid contents. Multivariate analytical techniques, including principal component analysis (PCA) and partial least squares (PLS) regression, were then used to investigate the correlation between the matrix composition and it spectral response. Our results indicate that the optical absorption of meniscus matrix is related to its composition, and this relationship is optimal in the NIR spectral range (750–1,100 nm). High correlations (R²_uronic = 86.9%, R²_water = 83.8%, R²_{hydroxyproline} = 81.7%, p < 0.0001) were obtained between the spectral predicted and measured meniscus composition, thus suggesting that spectral data in the NIR range can be utilized for estimating the matrix composition of human meniscus. In conclusion, optical spectroscopy, particularly in the NIR spectral range, is a potential method for evaluating the composition of human meniscus. This presents a promising technique for rapid and nondestructive evaluation of meniscus integrity in real‐time during arthroscopic surgery. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:270–278, 2016.     

NIR light-induced tumor phototherapy using photo-stable ICG delivery system based on inorganic hybrid

NIR responsive inorganic hybrid (Ti@GO) was synthesized. It could absorb NIR light and convert it into local hyperthermia and ROS synchronously. Ti@GO was firstly developed as a photosensitizer and a photothermal agent to realize tumor PTT and PDT. For anti-tumor application, HA was grafted on Ti@GO simultaneously as water solubility improver and tumor targeting moiety. ICG was chosen as a model drug. Results demonstrated that HA-Ti@GO could remarkably improve ICG stability and drug accumulation in 4T1 cells, enhance tumor phototherapy efficiency and reduce light-associated side effects. HA-Ti@GO/ICG under NIR laser irradiation showed a significant decreased cell viability of 20.7 ± 2.6% and a high DNA damage degree of 82.4 ± 8.3%. Moreover, in vivo results showed that HA-Ti@GO/ICG plus NIR laser achieved almost complete tumor regression on 4T1 tumor-bearing mice, with a tumor volume of 67.0 mm³. Taken together, our study provided a promising strategy to realize synergistic PTT/PDT tumor therapy with a single NIR light.     

Indocyanine green loaded hyaluronan-derived nanoparticles for fluorescence-enhanced surgical imaging of pancreatic cancer

Pancreatic ductal adenocarcinoma is highly lethal and surgical resection is the only potential curative treatment for the disease. In this study, hyaluronic acid derived nanoparticles with physico-chemically entrapped indocyanine green, termed NanoICG, were utilized for intraoperative near infrared fluorescence detection of pancreatic cancer. NanoICG was not cytotoxic to healthy pancreatic epithelial cells and did not induce chemotaxis or phagocytosis, it accumulated significantly within the pancreas in an orthotopic pancreatic ductal adenocarcinoma model, and demonstrated contrast-enhancement for pancreatic lesions relative to non-diseased portions of the pancreas. Fluorescence microscopy showed higher fluorescence intensity in pancreatic lesions and splenic metastases due to NanoICG compared to ICG alone. The in vivo safety profile of NanoICG, including, biochemical, hematological, and pathological analysis of NanoICG-treated healthy mice, indicates negligible toxicity. These results suggest that NanoICG is a promising contrast agent for intraoperative detection of pancreatic tumors.     

近红外光谱法测定头孢氨苄颗粒含量

目的 建立测定头孢氨苄颗粒含量的近红外光谱（NIR）快速分析方法。方法 以全国不同企业生产的187批头孢氨苄颗粒样品采集近红外光谱,分别建立校正集和检验集,校正集经内部交叉验证,建立校正模型,对检验集的样品进行分析。结果 头孢氨苄颗粒的浓度为0.042 0∽0.138 0 mg.mg 1,内部交叉验证决定系数（R2）为98.84,内部交叉验证均方差（RMSECV）为0.003,外部验证预测均方差（RMSEP）为0.003,预测值与真值的相关系数为0.995 0。结论 所建方法快速、简便、结果准确,可用于头孢氨苄颗粒的快速定量检验。     

NIR脉冲红外光技术与强脉冲光技术的临床联合应用

目的探讨NIR脉冲红外光技术与强脉冲光技术临床联合应用的疗效及安全胜。方法50例患者接受4～8次NIR脉冲红外光和强脉冲光联合治疗。于治疗前后采用照片对比,由医师对治疗效果进行综合评估,并对患者进行满意度评估。结果经4～6次治疗后,皮肤的亮度明显提高,皮肤松弛改善、色斑淡化、皱纹消除,患者对治疗效果非常满意。结论联合治疗技术有效地改善了皮肤质地及皮肤松弛和色斑的状况,皮肤变得嫩白,无不良反应发生。     

近红外光谱法快速分析小儿复方磺胺甲嗯唑颗粒的水分含量

目的利用近红外漫反射光谱（NIRDRS）分析技术和化学计量学方法对小儿复方磺胺甲嗯唑颗粒的水分含量进行快速定量分析。方法以全国不同企业生产的小儿复方磺胺甲嗯唑颗粒为分析对象,为扩大检测的浓度范围,通过恒温恒湿引湿的方法制得实验室制备样品,用光纤探头直接接触样品采集近红外漫反射光谱,采用偏最小二乘法（PLS）建立模型。结果小儿复方磺胺甲嗯唑颗粒水分定量分析模型由64个样本经内部交叉验证建立,42个样本用于外部验证,浓度范围为0．12％～6．15％,内部交叉验证相关系数（r）为0．9975,交叉验证均方差（RMSECV）为0．0835,外部验证均方差（RMSEP）为0．0865。结论建立的定量分析模型能对小儿复方磺胺甲嚷唑颗粒的水分含量进行准确、快速定量分析,方法简单可靠,可用于药品的现场快速分析。     

Near-infrared dye bound albumin with separated imaging and therapy wavelength channels for imaging-guided photothermal therapy

Development of theranostic agent for imaging-guided photothermal therapy has been of great interest in the field of nanomedicine. However, if fluorescent imaging and photothermal ablation are conducted with the same wavelength of light, the requirements of the agent's quantum yield (QY) for imaging and therapy are controversial. In this work, our synthesized near-infrared dye, IR825, is bound with human serum albumin (HSA), forming a HSA-IR825 complex with greatly enhanced fluorescence under 600 nm excitation by as much as 100 folds compared to that of free IR825, together with a rather high absorbance but low fluorescence QY at 808 nm. Since high QY that is required for fluorescence imaging would result in reduced photothermal conversion efficiency, the unique optical behavior of HSA-IR825 enables imaging and photothermal therapy at separated wavelengths both with optimized performances. We thus use HSA-IR825 for imaging-guided photothermal therapy in an animal tumor model. As revealed by in vivo fluorescence imaging, HSA-IR825 upon intravenous injection shows high tumor uptake likely owing to the enhanced permeability and retention effect, together with low levels of retentions in other organs. While HSA is an abundant protein in human serum, IR825 is able to be excreted by renal excretion as evidenced by high-performance liquid chromatography (HPLC). In vivo tumor treatment experiment is finally carried out with HSA-IR825, achieving 100% of tumor ablation in mice using a rather low dose of IR825. Our work presents a safe, simple, yet imageable photothermal nanoprobe, promising for future clinical translation in cancer treatment.