阿司匹林对人巨核细胞体外增殖的影响

目的:探讨阿司匹林对巨核细胞增殖、膜抗原表达及凋亡的影响.方法:将骨髓单个核细胞接种于含不同浓度阿司匹林的H2000培养基中,其中含Tpo 50ng·ml-1、SCF 50 ng·ml-1和IL-3 10ng·ml-1,5%CO2、37℃、饱和湿度的孵育箱中培养14d.结果:高浓度阿司匹林使巨核细胞的增殖变化明显高于低浓度,巨核细胞集落(CFU-MK)数减少,细胞丛(FFU-MK)数增加,CD41膜抗原表达率显著降低(P＜0.05);AnnexinV表达率明显增高(P＜0.05);DNA倍体分析的凋亡指数明显升高(P＜0.05);电镜观察可见出芽、空泡样结构和胞浆内颗粒减少.结论:高浓度阿司匹林可影响巨核细胞的正常代谢和促进其凋亡.     

养血升白口服液升高细胞机理研究

本文通过观察养血升口服液对白细胞减少小鼠脾细胞增殖，白介素Ⅱ活性及雄性大鼠血清睾酮浓度的影响来探讨其升高白细胞机理。     

林荫银莲花不定芽增殖培养基的优化

为提高林荫银莲花(Anemone flaccida Fr.Schmidt)不定芽繁殖系数,对其组培快繁体系进行优化。以林荫银莲花无菌苗为外植体,研究了培养基中激素、碳源、添加物和矿质元素钙等组分对其不定芽增殖和生长状况的影响,得出最佳的不定芽增殖培养基配方。以1/2 Ca浓度MS为固体培养基,激素为1.0 mg/L 6-BA和1.0 mg/L NAA,蔗糖或食用白糖30 g/L,水解酪蛋白为添加物,浓度为750 mg/L,培养30 d后繁殖系数可达4.01,大幅提高了不定芽的增殖速度和质量,为工厂化生产林荫银莲花种苗奠定了基础。     

膜联蛋白Ⅱ在乳腺癌组织中的表达以及对乳腺癌细胞侵袭的影响

目的探讨膜联蛋白Ⅱ在乳腺癌中的表达以及对乳腺癌细胞侵袭的影响。方法分别采用免疫组化方法检测AnnexinⅡ蛋白在正常乳腺组织、导管原位癌以及侵袭性导管癌组织中的表达情况,并测定高侵袭性乳腺癌细胞株及低侵袭性乳腺癌细胞株中AnnexinⅡm RNA(RT-PCR法)以及蛋白(蛋白印记技术)的表达情况。结果 AnnexinⅡ在正常乳腺组织中主要表达在间质内,在乳腺导管上不表达,在导管原位癌以及侵袭性导管癌组织中间质、血管、肿瘤组织上均有表达。AnnexinⅡ在正常乳腺组织表达的IOD值为(6.5±1.0)×106,在导管原位癌中表达的IOD值为(27.5±3.0)×106,在侵袭性导管癌组织中表达的IOD值为(40.0±11.0)×106。三组间比较,差异有高度统计学意义(P<0.01),导管原位癌、侵袭性导管癌组织中的表达量显著高于正常乳腺组织,而侵袭性导管癌中的表达量显著高于导管原位癌(均P<0.01)。AnnexinⅡm RNA在高侵袭性人乳腺癌细胞株MDA-MB-435s中的表达灰度值(0.83±0.11)显著高于MCF-7株中的表达(0.07±0.01),差异有高度统计学意义(P<0.01);AnnexinⅡ蛋白在高侵袭性人乳腺癌细胞株MDA-MB-435s中的表达(1.47±0.08)显著高于MCF-7株中的表达(0.11±0.03),差异有高度统计学意义(P<0.01)。结论 AnnexinⅡ在乳腺癌中高表达,并且瘤细胞侵袭性越强,其表达也越强。     

Event-related brain potentials uncover activation dynamics in the lexicon of multiplication facts

The present study was designed to ascertain whether the cortical dynamics underlying multiplication fact retrieval can be modulated as a function of arithmetic relatedness. To this end, event-related brain potentials (ERPs) were recorded from 22 participants engaged in a number-matching task. The task was to decide whether a single probe number had been shown as part of a previously presented pair of cue numbers. Probes in the non-matching condition (50% of total trials) were either the product of the cue numbers (strong cue-probe association), the multiple of either cue numbers (weak cue-probe association), or an arithmetically neutral number with respect to the cue numbers (no cue-probe association). Behavioral data showed a clear interference effect (LeFevre interference), in that performance in the non-matching condition was significantly better when the probe number was arithmetically neutral compared to when it was arithmetically related to the cue digits either strongly (i.e., through the product) or weakly (i.e., through its neighbor in the multiplication table). LeFevre interference was not statistically different in magnitude for the two arithmetically related conditions. In contrast, ERPs allowed us to dissociate cortical processing dynamics for these latter conditions: whereas initially (250-350 ms) both product and neighbor probes evoked a relatively more pronounced positivity compared to neutral probes, in a later interval (350-450 ms, N400-like component), neighbors critically diverged from products, showing relatively more negative values, similar to those of neutral probes. The observed dissociation in ERP measures is interpreted as evidence of activation spreading in the network of multiplication facts, with the short-lasting response elicited by neighbors likely reflecting activation dissipating over time because of a weaker association to the cue digits.     

痤疮丙酸杆菌活菌对小胶质细胞增殖和凋亡的影响

目的探讨外伤后细菌性致死性肉芽肿病原菌一痤疮丙酸杆菌对小胶质细胞（MG）增殖、凋亡的影响。方法病原菌以不同浓度和不同时间作用于小胶质细胞，采用甲基噻唑基四唑（MTT）法检测细胞活性，流式细胞仪检测细胞凋亡和细胞周期，并用光镜和电镜观察细胞形态学变化。结果经痤疮丙酸杆菌作用后小胶质细胞活性明显受到抑制，呈时间依赖性，结果具有统计学意义（P〈0．05）。痤疮丙酸杆菌可诱导小胶质细胞的凋亡，凋亡率在实验组和对照组之间存在显著差异（P〈0．05），但细胞周期检测发现实验组与对照组无明显差别。结论外伤后细菌性致死性肉芽肿病原菌．痤疮丙酸杆菌可抑制小胶质细胞的生长，诱导小胶质细胞的调亡，可能和患者的病原菌入颅后导致的炎症反应密切相关。     

Züchtung und Vermehrung von Herpes simplex-Virus in Zellkulturen vom Nervensystem

Zusammenfassung Die häufig diskutierte, (Mit)beteiligung von Herpes simplex-Virus (HSV) bei bestimmten Alterationen im ZNS sowie der hohe Neutropismus des HSV führte zur Frage, inwieweit sich HSV auch in in vitro gezüchteten, spezifischen Zellen vom Nerven-system vermehren und züchten läßt, und ob sich hierbei licht-und elektronenmikroskopisch charakteristische Alterationen nachweisen lassen.Es zeigte sich, daß sich das HSV in allen verschiedenen angezüchteten spezifischen Zellen (von Plexus chorioideus des Rhesusaffen, Kaninchenspinalganglien, menschlichen Oligodendrogliomen, Meningeomen sowie fibrillären und protoplasmatischen Astrocytomen) kultivieren und vermehren läßt. Hierbei kommt es in den spezifischen Zellen nicht nur lichtmikroskopisch zu charakteristischen cytopathischen Veränderungen, sondern auch biologisch zu einer signifikanten Vermehrung des Virus in diesen Zellen. Auch elektronenmikroskopisch lassen sich viele der charakteristischen Bildungs-, Reifungs- und Ausschleusungsstadien des Virus in den Kernen und im Cytoplasma der Zellen beobachten. Die Besonderheiten der elektronenmikroskopisch erhobenen ultrastrukturellen Befunde werden ausführlich beschrieben, und die sich daraus abzuleitenden Möglichkeiten werden im Hinblick auf die biologischen Eigenheiten des HSV bei in vivo-Infektionen, aber auch im Hinblick auf die Deutung lichtmikroskopisch histochemischer und immunhistologischer Befunde nach in vivo-Infektionen mit diesem Virus ausführlich diskutiert.

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Cultivation and multiplication of herpes simplex virus in cell cultures of the nervous system

Summary The participation of herpes simplex virus (HSV) in certain alterations of the CNS has been partially questioned and frequently discussed. Therefore, the question arose whether HSV can also be cultivated and multiplied in vitro in CNS specific cells, and if so, whether characteristic alterations and structures can be observed by means of light and electron-microscopic examinations.Our data demonstrate that HSV multiplies in all different specific cells originating from plexus chorioideus of rhesus monkeys, from spinal ganglia of rabbit, from human oligodendroglioma, meningeoma as well as from fibrillar and protoplasmatic astrocytoma. Characteristic cytopathic alterations of these specific cells and significant multiplication of the virus in these cells are to be found. Many of the characteristic forms of formation, maturation and release of HSV can be seen by electronmicroscopic examinations. The particular ultrastructural data observed by electron microscopy are described in detail and the resulting possibilities are broadly discussed not only with regard to the biologic particularity of HSV in in vivo infection but also in view of the interpretations, deriving from histochemical and immunohistological data obtained by light microscopy after in vivo infection.

     

The operand-order effect in single-digit multiplication: an ERP study of Chinese adults

Unlike those used in the West, a typical Chinese multiplication table includes only smaller-operand-first entries (e.g., 3 x 7=21, but not 7 x 3=21). Due to this unique feature, multiplication for Chinese subjects has been found to show an operand-order effect. The present study aims to investigate the neural bases of the operand-order effect. Subjects were 20 Mainland Chinese subjects who learned as children the half multiplication table (i.e., smaller-operand-first entries only) and 20 Hong Kong and Macao Chinese subjects who learned as children the whole multiplication table (i.e., both smaller- and larger-operand-first entries) under the British and Portuguese educational systems, respectively. ERP data showed that, for those who learned the half table (Mainland Chinese), but not for those who learned the whole table (Hong Kong and Macao Chinese), the larger-operand-first problems elicited greater negative potentials across representative electrodes of the whole scalp, emerging at about 120 ms after the onset of the second operand and lasting until around 750 ms. These results suggest that the particular experience of acquiring multiplication facts had pronounced impact on their representations in the brain.