HPLC法对不同产地两头尖中皂苷D的定量分析

　　Using raddeanoside D, the main active constituent of Anemone raddeana Regel, as the quality control criteria, 10 samples of the herbal drug collected from different localities of Heilongjiang, Jilin, Liaoning and Shandong provinces were analysed by HPLC. Results showed that the average raddeanoside D contents were 0.355 0%, 0.321 3%, 0.280 7% and 0.219 6% respectively. This method was proved to be quick, simple and reproducible, and provided a reliable basis for the quality control and evaluation of A.raddeana.     

Metastatic Epithelioid Gastrointestinal Stromal Tumor: Yet Another Tumor with Anemone Cell Features

Since 1980 when Sibley and coworkers first described a nodal neoplasm of unknown histogenesis with striking surface microvilli for which they introduced the term "anemone cell," a series of reports have appeared in the literature illustrating tumors with similar ultrastructural features. While most reported cases showed differentiation along a particular line, rare cases remained histogenetically unclear. In this report a case is described of epithelioid gastric gastrointestinal stromal tumor metastatic to the liver, showing conspicuous long microvillus-type cell processes partially or circumferentially coating the cell surfaces, thus qualifying as yet another tumor type with anemone cell features.     

蜈蚣三七的化学成分及免疫抑制活性

目的:研究毛茛科银莲花属植物林荫银莲花(Anemone flaccida Fr.schmjdt)的根茎蜈蚣三七的化学成分.方法:利用硅胶柱和C18色谱柱进行分离纯化,根据理化性质和光谱数据鉴定化合物的结构.MTT法评价各化合物的免疫抑制活性.结果:分离并鉴定了5个化合物:S1-Go-2(I),hemsgiganosides B(II),anhuienosides E(III),hederasaponin B(IV)和flaccidoside II(V).化合物Ⅲ,Ⅳ,V对体外淋巴细胞增殖有抑制作用.结论:化合物I,Ⅱ,Ⅳ为首次从银莲花属植物中分离得到,化合物Ⅲ为首次从林荫银莲花中分离得到.化合物Ⅲ,Ⅳ,V具备免疫抑制活性.     

九节菖蒲的化学成分

目的：研究九节菖蒲的化学成分。方法：对九节菖蒲乙醇提取物的氯仿萃取部分进行色谱分离，根据光谱数据和理化性质确定各化合物的结构。结果：分离并鉴定了7个化合物，分别为；三十烷（1），棕榈酸（2），β-谷甾醇（3），异阿魏酸（4），鸡脚刺醛（5），5-羟基乙酰丙酸（6），胡萝卜苷（7）。结论：化合物4和5为首次从本属植物中分离得到。     

HPLC法对不同产地两头尖中皂苷D的定量分析

　　Using raddeanoside D, the main active constituent of Anemone raddeana Regel, as the quality control criteria, 10 samples of the herbal drug collected from different localities of Heilongjiang, Jilin, Liaoning and Shandong provinces were analysed by HPLC. Results showed that the average raddeanoside D contents were 0.355 0%, 0.321 3%, 0.280 7% and 0.219 6% respectively. This method was proved to be quick, simple and reproducible, and provided a reliable basis for the quality control and evaluation of A.raddeana.     

安徽银莲花化学成分的研究(Ⅰ)

从安徽银莲花的根茎中提取、分离出9个化合物,经化学方法和光谱分析,鉴定了4个化合物,化合物Ⅰ为齐墩果酸(oleanolic acid),化合物Ⅱ为胡萝卜甙(daucosterol),化合物Ⅲ为齐墩果酸-3-O-α-L-吡喃鼠李糖-(1→2)-β-D-吡喃木糖甙,化合物Ⅳ为齐墩果酸-3-O-β-D-吡喃葡萄糖-(1→2)-β-D-吡喃木糖甙。以上化合物均为首次从该植物中分离得到。     

林荫银莲花不定芽增殖培养基的优化

为提高林荫银莲花(Anemone flaccida Fr.Schmidt)不定芽繁殖系数,对其组培快繁体系进行优化。以林荫银莲花无菌苗为外植体,研究了培养基中激素、碳源、添加物和矿质元素钙等组分对其不定芽增殖和生长状况的影响,得出最佳的不定芽增殖培养基配方。以1/2 Ca浓度MS为固体培养基,激素为1.0 mg/L 6-BA和1.0 mg/L NAA,蔗糖或食用白糖30 g/L,水解酪蛋白为添加物,浓度为750 mg/L,培养30 d后繁殖系数可达4.01,大幅提高了不定芽的增殖速度和质量,为工厂化生产林荫银莲花种苗奠定了基础。     

两头尖中的香豆素类成分及其生物活性研究

为了研究两头尖的香豆素类化学成分, 利用硅胶柱色谱及高效液相色谱等方法对两头尖提取物进行分离和纯化, 根据理化性质和光谱数据鉴定化合物结构。从中分离得到两个新的香豆素类化合物, 分别鉴定为4, 7-二甲氧基-5-甲基-6-羟基香豆素 (1) 和4, 7-二甲氧基-5-醛基-6-羟基香豆素 (2)。生物活性试验显示两个化合物对肿瘤细胞的增殖有抑制作用, 对维甲酸α受体 (RARα) 有激动活性, 同时对人白细胞弹性蛋白酶也有抑制作用。     

Chemical constituents from rhizome of Anemone amurensis

Phytochemical investigation of the 70% EtOH extract of the rhizome of Anemone amurensis led to the isolation of two new oleanane-type triterpenoid saponins 1 and 2. Their structures were elucidated on the basis of chemical and spectral analysis, including 1D, 2D NMR data, and HR–ESI–MS. Compounds 1 and 2 were tested for cytotoxicities against two human cancer cell lines (A549 and Hep-G2). Compound 2 showed potent cytotoxicity with IC₅₀ values of 38.53 and 66.17 μM, respectively, while compound 1 with IC_50?>?100 μM.     

基于GMPGIS的地乌全球产地生态适宜性分析

目的：本研究在药用植物全球产地生态适宜性区划信息系统（GMPGIS）的支持下,对鹅掌草全球生态适宜性进行了分析。方法：以地乌药材原植物鹅掌草道地产区、主产区和野生分布区的195个分布数据为基点,选取年均温、年均相对湿度、年均降水量、年均日照、土壤类型等7个生态指标作为主要影响因子。结果：鹅掌草全球生态相似度最大的区域主要分布在美国、俄罗斯、加拿大、中国、澳大利亚、哈萨克斯坦、墨西哥、阿根廷、伊朗、巴西、土耳其、乌克兰、南非等国。其中,鹅掌草在中国的生态适宜性地区主要包括陕西、贵州、甘肃、四川、湖北、重庆、浙江、云南、江苏、湖南等省区。结论：本研究表明了GMPGIS系统用于药用植物产地生态适宜性分析的科学性和重要性,并为地乌药材的引种以及合理规划生产布局提供了参考依据。