茶多酚通过调节Akt通路诱导胰腺癌细胞凋亡

Objective To investigate the regulation of Akt signaling pathway involved in apeptosis of Panc-I cells induced by polyphenol ( - )-epigallocatechin-3-gallate. Methods Pane-1 cells were maintained in DMEM with 10% fetal calf serum in a 95% air,5% CO2 atmosphere,then incubated in different concentrations of EGCG (6.3,12.5,25.0,50.0 μmol/L). The growth arrest effect was determined by MTT assay, and apoptosis was detected by agarose gel electrophoresis. The levels of Akt (60×103 ) and Bad (23×103) were determined by Western blot. Results EGCG time and dose dependently repressed the growth of Panc-1 cells and induced apeptosis in Panc-1 cells ( P <0.05 ). The expression of p-Akt (set473) and p-Bad (ser136) was reduced in a dose-dependent way,whereas the pan-Akt and pan-Bad were unchanged (P < 0.05). Conclusion EGCG can induce apoptosis in pancreatic cancer cells in a dose-and time-dependent fashion by attenuating the blocking effects of Akt signaling pathway on Bad proteins.     

“石话石说”养生经

中国人对石头的感情自古以来深厚而绵延,无论是凝练了劳动人民智慧的石具,还是点缀于各大名著之中的"石缘",石头成就了中国石器时代的文明,成就了诸如《石头记》一样的文学奇葩,甚至成就了独特的石文化.中国也因此有了"山无石不奇,水无石不清,园无石不秀,室无石不雅,宅无石不安,画无石不名,商无石不发"的爱石情结.但除此之外,石头还有一项特质容易被现代人忽略,那就是养生保健的特质,甚至可以说"人无石不寿",因为石头的养生保健功能的确不少.     

生气不如争气

1宋徽宗喜欢书画,并且有很深的造诣。一天,他问随从:"天下何人画驴最好?"随从四处打听,匆忙中得知一位叫朱子明的画家有"驴画家"之称,即召他进宫画驴。得知被召进宫是为皇上画驴时,朱子明吓出一身冷汗。他原本是个很有功底的山水画家,可同行们嫉     

茶多酚通过调节Akt通路诱导胰腺癌细胞凋亡

Objective To investigate the regulation of Akt signaling pathway involved in apeptosis of Panc-I cells induced by polyphenol ( - )-epigallocatechin-3-gallate. Methods Pane-1 cells were maintained in DMEM with 10% fetal calf serum in a 95% air,5% CO2 atmosphere,then incubated in different concentrations of EGCG (6.3,12.5,25.0,50.0 μmol/L). The growth arrest effect was determined by MTT assay, and apoptosis was detected by agarose gel electrophoresis. The levels of Akt (60×103 ) and Bad (23×103) were determined by Western blot. Results EGCG time and dose dependently repressed the growth of Panc-1 cells and induced apeptosis in Panc-1 cells ( P <0.05 ). The expression of p-Akt (set473) and p-Bad (ser136) was reduced in a dose-dependent way,whereas the pan-Akt and pan-Bad were unchanged (P < 0.05). Conclusion EGCG can induce apoptosis in pancreatic cancer cells in a dose-and time-dependent fashion by attenuating the blocking effects of Akt signaling pathway on Bad proteins.     

石子“跑”掉了

去年秋天,单位组织职工体检,除了体重超标,B超还在我的右肾里查出一颗0.2×0.2厘米的结石。虽然没有感到任何不适症状,但有这个"定时炸弹"潜伏在身体里,着实让我吃不香、睡不好。尤其是听了有些以前做过开刀或体外冲击波碎石手术的同事讲起手术时的疼痛感,更是令我紧张。好在医生给了我一个很好     

你是我的幸福石

时间是链子,快乐是珠子,用链子串上珠子挂在胸前就可以幸福一辈子。幸福是石子,烦恼是沙子,时间是筛子,用筛子漏掉沙子,石子就会陪你一辈子。     

影响薄片法血小板聚集实验的因素

目的:研究常见影响因素对薄片法血小板聚集实验(SPAT)时间的影响.方法:测定下列富含血小板血浆(PRP)的SPAT:①调整血小板计数分别为240,120,60,30和15×109/L的8例健康献血者PRP;②分别在40,35,30,25,20,15,10和5℃条件下恒温30 min的10例健康献血者PRP;③含终浓度为0,10,20,30,40和50g/L的二甲基亚砜(DMSO)并室温放置30 min的10例健康献血者PRP;④在室温放置1,2,3,4 h后的8例健康志愿者服用阿司匹林前后PRP;⑤含浓度分别为0,0.5,1.0,2.0和3.O U/L的肝素或0.1 g/LEDTA的8例健康献血者PRP.结果:①随着血小板计数的降低,SPAT时间逐渐延长;同一供者血小板计数与其SPAT时间呈显著的直线负相关(r=0.996,P=0.004);②温度在20～40℃对SPAT时间无明显影响,但当温度低于20℃时SPAT时间明显延长;③DMSO对SPAT有明显的影响,使SPAT时间明显延长,并呈明显的剂量效应;④血小板标本室温放置1,2,3和4 h SPAT无显著差异(P=0.815);⑤肝素对SPAT时间无明显影响,而0.1 g/L乙二胺四乙酸(EDTA)可完全抑制血小板聚集.结论:血小板计数、所处的温度以及含有DMSO时,均能在一定程度上影响SPAT时间,但是上述因素均不影响血小板最终形成肉眼可见的聚集,且聚集时间仍然在180 s以内.     

踩石子,踩出脑健康

现在,在许多大中城市里,中老年健身爱好者掀起了一股光脚踩石子的热潮。每天,在一些公园的林荫道上,许多市民纷纷脱下鞋子在铺着石子的路上来回走着,那种又痛又痒的感觉,令他们特别惬意。不仅如此,     

过氧化氢诱导体外损伤性白内障模型的构建及晶状体上皮细胞的凋亡

背景：氧化应激能够诱导晶状体上皮细胞发生凋亡。 目的：观察Fas蛋白与过氧化氢诱发白内障中晶状体上皮细胞凋亡的关系以及表没石子儿茶素没食子酸酯对Fas蛋白表达及晶状体上皮细胞凋亡的影响。 方法：将健康成年兔透明晶状体随机分为3组：空白对照组仅加入DMEM培养液,过氧化氢组加入DMEM+过氧化氢,表没石子儿茶素没食子酸酯组加入DMEM+过氧化氢+表没石子儿茶素没食子酸酯。 结果与结论：各组体外培养72 h后过氧化氢组晶状体上皮细胞凋亡率及Fas蛋白阳性表达率显著高于空白对照组(P < 0.05)。表没石子儿茶素没食子酸酯组晶状体上皮细胞凋亡率及Fas蛋白阳性表达率显著低于过氧化氢组(P < 0.05)。结果显示,过氧化氢可能是通过上调Fas蛋白的表达诱导晶状体上皮细胞凋亡,而抗氧化剂表没石子儿茶素没食子酸酯可下调Fas蛋白的表达而减轻晶状体上皮细胞的凋亡。     

握住自己的命运

从前，一位国王准备处死一名使节。为显示自己的公正，那天，国王召集了很多民众，并当众拿出了一个布袋，说布袋里有两粒石子，一粒是白石子，一粒是黑石子，现在由使节自己决定自己的命运，如果他从布袋里摸出了一粒白石子，便可无罪释放；如果摸出的是一粒黑石子，便会立即处死。