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1.
运动性中枢疲劳及乳酸对大鼠大脑皮质神经细胞MCT mRNA表达的影响 总被引:3,自引:0,他引:3
目的:探讨运动性中枢疲劳时大鼠大脑皮质MCT1、MCT2mRNA表达的变化以及乳酸对大鼠大脑皮质神经元MCT2mRNA表达的影响。方法:12只Wistar大鼠随机分为对照组和力竭训练1周组,每组6只。通过7天力竭训练建立中枢疲劳模型,观察中枢疲劳时大鼠大脑皮质MCT1、MCT2mR-NA表达变化;采用原代培养的大鼠大脑皮质神经元,观察不同浓度乳酸作用后大鼠大脑皮质神经元MCT2mRNA表达的变化。采用Trizol法提取大脑皮质和神经元总RNA;用SYBRAgreenI荧光定量RT-PCR测定MCT1及MCT2mRNA表达变化。结果:整体水平:力竭训练1周组MCT1mRNA表达无明显变化,而MCT2mRNA表达较对照组显著升高6·35±3·54倍(P<0·05)。细胞水平:乳酸作用后,神经元MCT2mRNA表达在pH=7·25时较对照组(pH=7·35组)升高3·08±2·38倍(P<0·05)。但随着乳酸浓度进一步升高,MCT2mRNA表达无明显增加。结果提示,中枢疲劳可能引发大脑皮质神经元MCT2mRNA表达升高,MCT2基因表达可能与乳酸在中枢疲劳中的作用有关。 相似文献
2.
最小二乘法在异常点存在时,其回归方程的“稳健性”往往受到影响。本文利用中位数比平均数稳健这一性质,给出一种可抗异常点干扰的一元稳健回归方程的求解方法。 相似文献
3.
陈忠祥 《中国现代应用药学》2005,(1):45-45
在分析工作中 ,经常遇到处理 2个变量之间关系的问题。比如分光光度法中吸光度与溶液浓度之间的关系 ,在这2个变量中 ,被测浓度我们称自变量 ,用 x表示 ;吸光度我们称因变量 ,用 y表示。因变量 y随自变量 x变化呈现规律性 ,为减小误差 ,可用一元线性回归方程 y=a+bx来处理这 2个变量之间的关系。分光光度法中通常是用已知溶液测定吸光度 ,通过几组测定数据制作标准曲线。测定待测溶液吸光度 ,从标准曲线上查得该溶液浓度 ,通过计算便可知试样含量。但是绘制标准曲线繁杂 ,加之所测的数据难免有偏离曲线的点 ,因而导致误差。用一元线性回归… 相似文献
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目的:实现一种应用于生物化学分析仪的定量分析方法。方法:利用一元一次线性回归模型,结合标准曲线来计算待测样本的浓度值。结果:经过验证和临床测试,该算法可满足性能要求,且有效可行。结论:该方法能很好地满足临床应用,并能扩展到工业检测、食品安全等领域。 相似文献
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双波长一元线性回归分光光度法同时测定复方氨基比林注射液中三组分含量 总被引:3,自引:0,他引:3
本文依据一组含有不同比例待测和干扰组分的标准混合液的吸收值,采用一元线性回归方法,在选择最佳测定波长对的同时建立标准工作曲线方程,使其更符合实际作品测定时的情况,提高了结果的精度和可靠性,并使计算量和实验工作量得以降低。应用于复方氨基比林注射液中三组分氨基比林、安替比林和巴比妥的同时测定,其平均回收率分别为99.8%,100.4%和99.8%,变异系数分别为0.59,1.48和1.05,结果优于卡尔曼滤波法、偏最小二乘法和目标因子分析法。 相似文献
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一元制剂二氧化氯消毒粉剂杀菌性能及毒性试验研究 总被引:5,自引:4,他引:5
一元制剂二氧化氯消毒粉剂是由3种主要成分组成,为了解其杀菌效果和毒性,采用悬液定量杀菌试验和动物毒性试验进行了实验室研究。结果,以含30 mg/L二氧化氯该消毒剂水溶液对悬液内大肠杆菌作用5 m in,平均杀灭率为99.95%;15 mg/L二氧化氯消毒液对金黄色葡萄球菌作用5 m in,平均杀灭率为99.91%;60 mg/L二氧化氯消毒液对枯草杆菌黑色变种芽孢作用10 m in,平均杀灭率为99.94%。以200 mg/L二氧化氯消毒液作用5m in,可完全破坏悬液内HBsAg的抗原性。菌悬液内含体积分数25%以上小牛血清对其杀灭大肠杆菌效果有轻度影响。该二氧化氯消毒粉剂对小鼠急性经口LD50值大于5000 mg/kg,属无毒类物质;以100 mg/L二氧化氯消毒液对家兔皮肤刺激试验指数为0.63,属于轻度刺激性。结论,一元制剂二氧化氯消毒粉剂对细菌繁殖体和细菌芽孢具有良好的杀菌效果,动物毒性试验属无毒类物质、对家兔皮肤有轻度刺激性。 相似文献
9.
Correlation between changes of central neurotransmitter expression and stress response in mice*☆
A restraint time-course analysis 总被引:1,自引:0,他引:1
BACKGROUND: Changes in central neurotransmitter expression play an important role in stress response and forms the basis for stress-induced psychological and behavior changes.
OBJECTIVE: To observe the effects of different restraint stress intervals on brain monoamine neurotransmitter expression, and to investigate the correlation between stress response and neurotransmitter levels.
DESIGN: Randomized controlled animal study.
SETTING: Chinese Herb and Natural Medicine Institute, Pharmacological College of Jinan University.
MATERIALS: Sixty 7-week-old male Kunming mice of clean grade, weighing 18-22 g, were provided by the Guangdong Medical Experimental Animal Center. The experiment was in accordance with animal ethics standards.
METHODS: This study was performed at the Chinese Herb and Natural Medicine Institute, Pharmacological College of Jinan University from June 2006 to May 2007. A restraint device for mice was constructed according to published reports. Experimental mice were adaptively fed for 1 week and randomly divided into a control group (n = 10) and an experimental group (n = 50). The experimental group was sub-divided into five restraint intervals: 4, 8, 12, 18, and 24 hours (n = 10 mice per time point). Animals in the experimental group were not allowed to eat or drink during the restraint period. Mice in the control group did not undergo restraint, but had identical food and water restrictions. Cerebral cortex and hypothalamus were separated based on observational times and protein was extracted using perchloric acid. Central monoamine neurotransmitter levels were measured using high performance liquid chromatography with electrochemical detection. MAIN OUTCOME MEASURES: Levels of norepinephrine (NE), dopamine hydrochloride (DA), 3,4-dihydroxyphen-ylanetic acid (DOPAC), homovanillic acid (HVA), 5-hydroxytryptamine (5-HT), and 5-hydroxyindoleac-etic acid (5-HIAA) in the cerebral cortex and hypothalamus of mice. RESULTS: Sixty mice were i 相似文献
OBJECTIVE: To observe the effects of different restraint stress intervals on brain monoamine neurotransmitter expression, and to investigate the correlation between stress response and neurotransmitter levels.
DESIGN: Randomized controlled animal study.
SETTING: Chinese Herb and Natural Medicine Institute, Pharmacological College of Jinan University.
MATERIALS: Sixty 7-week-old male Kunming mice of clean grade, weighing 18-22 g, were provided by the Guangdong Medical Experimental Animal Center. The experiment was in accordance with animal ethics standards.
METHODS: This study was performed at the Chinese Herb and Natural Medicine Institute, Pharmacological College of Jinan University from June 2006 to May 2007. A restraint device for mice was constructed according to published reports. Experimental mice were adaptively fed for 1 week and randomly divided into a control group (n = 10) and an experimental group (n = 50). The experimental group was sub-divided into five restraint intervals: 4, 8, 12, 18, and 24 hours (n = 10 mice per time point). Animals in the experimental group were not allowed to eat or drink during the restraint period. Mice in the control group did not undergo restraint, but had identical food and water restrictions. Cerebral cortex and hypothalamus were separated based on observational times and protein was extracted using perchloric acid. Central monoamine neurotransmitter levels were measured using high performance liquid chromatography with electrochemical detection. MAIN OUTCOME MEASURES: Levels of norepinephrine (NE), dopamine hydrochloride (DA), 3,4-dihydroxyphen-ylanetic acid (DOPAC), homovanillic acid (HVA), 5-hydroxytryptamine (5-HT), and 5-hydroxyindoleac-etic acid (5-HIAA) in the cerebral cortex and hypothalamus of mice. RESULTS: Sixty mice were i 相似文献
10.