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Jing Li Lian-mei Zhao Cong Zhang Meng Li Bo Gao Xu-hua Hu Jian Cao Gui-ying Wang 《Oncology research》2020,28(1):51-63
Long noncoding RNAs (lncRNAs) participate in and regulate the biological process of colorectal cancer (CRC)
progression. Our previous research identified differentially expressed lncRNAs in 10 CRC tissues and 10
matched nontumor tissues by next-generation sequencing (NGS). In this study, we identified an lncRNA,
FEZF1 antisense RNA 1 (FEZF1-AS1), and further explored its function and mechanism in CRC. We verified
that FEZF1-AS1 is highly expressed in CRC tissues and cell lines. Through functional experiments, we found
that reduced levels of FEZF1-AS1 significantly suppressed CRC cell migration, invasion, and proliferation
and inhibited tumor growth in vivo. Mechanistically, we discovered that reduced levels of the lncRNA FEZF1-
AS1 inhibited the activation of epithelial–mesenchymal transition (EMT); the overexpression of orthodenticle
homeobox 1 (OTX1) partially rescued the FEZF1-AS1-induced inhibition of protein expression. It indicated
that FEZF1-AS1 may play a role in the occurrence and development of CRC by regulating the FEZF1-AS1/
OTX1/EMT pathway. Furthermore, it was reported that FEZF1-AS1 is located in both the nucleus and cytoplasm of HCT116 cells. Dual-luciferase reporter assays verified that FEZF1-AS1 directly binds miR-30a-5p
and negatively regulated each other. Further, we showed that 5 -nucleotidase ecto (NT5E) is a direct target of
miR-30a-5p, and the inhibition of miR-30a-5p expression partially rescued the inhibitory effect of FEZF1-AS1
on NT5E. Our results indicated that the mechanism by which FEZF1-AS1 positively regulates the expression
of NT5E is through sponging miR-30a-5p. Our study demonstrated that lncRNA FEZF1-AS1 is involved in the
development of CRC and may serve as a diagnostic and therapeutic target for CRC patients. 相似文献
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