一阶导数光谱法测定维甲酸凝胶的含量

目的：建立维甲酸凝胶的质控方法。方法：采用一阶导数光谱法在378nm处测定维甲酸的含量。结果：维甲酸的平均回收率为100.37％。结论：所建立的方法适用于维甲酸凝胶剂的含量测定。     

视黄酸依赖反义cyclin D1对HL-60细胞增殖和分化的影响及其机制

目的 :探讨视黄酸 (retinoicacid ,RA)及其诱导的反义 (anti sense ,AS)cyclinD1对肿瘤细胞的协同抑制增殖与诱导分化效应及其机制。方法 :通过构建的含有视黄酸反应元件 (retinoicacidresponseelement ,RARE)的反义cyclinD1RNA表达载体 pCI neo/RARE3 TK/AscyclinD1,使反义cyclinD1的表达可受RA诱导调控。以脂质体转染HL 60白血病细胞后 ,运用生长曲线、MTT比色法和流式细胞仪分析检测细胞增殖功能 ,NBT还原实验和透射电镜观测细胞分化状况 ,western blot检测p16基因蛋白表达水平。结果 :反义cy clinD1转染和未转染的HL 60细胞经RA处理后 ,生长减慢 ,细胞被阻滞在G0 /G1期 ,分化能力明显增强 ,p16基因蛋白表达增加 ,其中 ,以反义cyclinD1转染细胞经RA处理后变化更为明显。结论 :RA及其诱导的反义cyclinD1可协同抑制HL 60细胞增殖和诱导细胞分化成熟 ,上调p16基因表达可能是其分子机制之一。     

Rare labial ulcer related to the use of all‐trans retinoic acid in a patient with acute promyelocytic leukemia

Acute promyelocytic leukemia (APL) is a subtype of acute myeloid leukemia (AML). All‐trans retinoic acid (ATRA) is the first‐choice therapy for the treatment of this disease, but has been associated with side effects, the most serious of which is retinoic acid syndrome (RAS). RAS is characterized by unexplained fever, dyspnea, pulmonary infiltrate, leukocytosis and nephropathy. Genital ulcers have been described in some cases, but only two cases of oral ulcers related to this syndrome have been described in the literature. This paper describes the third case of oral ulceration related to ATRA in a 32‐year‐old white man with diagnosis of APL. Clinicians should know the side effects of ATRA and identify oral ulcers resulting from this therapy. The prompt identification of these ulcers enables the institution of appropriate treatment and can therefore contribute to continuation of the patient's cancer treatment.     

A case of all‐trans retinoic acid‐induced myositis in the treatment of acute promyelocytic leukaemia

The use of all‐trans retinoic acid (ATRA) is now standard therapy for the treatment of acute promyelocytic leukaemia (APML). There have been increasing reports of ATRA‐induced myositis, with its frequent association with retinoic acid syndrome and Sweet's syndrome. We report a case of a young man with APML who developed ATRA‐induced myositis characterized by unexplained fevers, bilateral leg swelling and a non‐painful purpuric, petechial rash, with prompt resolution of symptoms and signs with high‐dose steroids and cessation of ATRA. Rapid recognition of this adverse reaction and prompt institution of steroids is of prime importance given its potentially fatal course.     

Spin基因在维甲酸诱导神经管缺损的表达验证

目的探讨印讥基因在神经管缺损的表达及其作用机制。方法用中密度芯片基因比较正常小鼠与神经管缺损小鼠胚胎9．5、10．5d时神经管组织中Spin基因的表达差异,并经Northern杂交验证。结果通过比较发现：印讯基因在正常神经管组织表达上调趋势（Cy5／Cy3〉2．0）,而在维甲酸诱导的神经管缺损组织中呈现下调趋势（Cy5／Cy3〈0．5）。结论Spin基因参与神经管缺损发生和发展过程,可能在神经管异常发育中具有重要作用,对该基因的研究有助于阐释神经管缺损的发病机制。