云南拉祜族六个基因座的遗传多态性研究

目的：了解云南拉祜族６ 个短串联重复序列基因座遗传多态性分布。方法：应用复合扩增技术对ＣＳＦ１ＰＯ、ＴＰＯＸ、ＴＨ０１ 、Ｆ１３Ａ０１、ＦＥＳＦＰＳ和ｖＷＡ等６ 个ＳＴＲ基因座进行分析,采用高分辨率的聚丙烯酰胺凝胶电泳分离、银染法显影技术,对云南拉祜族上述６ 个ＳＴＲ基因座进行遗传多态性调查。结果：ＣＳＦ１ＰＯ基因座,观察到８ 个等位基因,２０ 个基因型;ＴＰＯＸ 基因座,观察到５ 个等位基因,１４ 个基因型;ＴＨ０１ 基因座,观察到６ 个等位基因,１７ 个基因型;Ｆ１３Ａ０１ 基因座,观察到６ 个等位基因,１３ 个基因型;ＦＥＳＦＰＳ基因座,观察到７ 个等位基因,１５ 个基因型;ｖＷＡ基因座,观察到７ 个等位基因,１７ 个基因型。结论：上述６ 个短串联重复序列基因座基因频率分布与Ｈａｒｄｙ Ｗｅｉｎｂｅｒｇ 平衡吻合良好。     

Endoscopic anti-reflux therapy for gastroesophageal reflux disease

Gastroesophageal reflux disease has an increasing incidence and prevalence worldwide. A significant proportion of patients have a suboptimal response to proton pump inhibitors or are unwilling to take lifelong medication due to concerns about long-term adverse effects. Endoscopic anti-reflux therapies offer a minimally invasive option for patients unwilling to undergo surgical treatment or take lifelong medication. The best candidates are those with a good response to proton pump inhibitors and without a significant sliding hiatal hernia. Transoral incisionless fundoplication and nonablative radiofrequency are the techniques with the largest body of evidence and that have been tested in several randomized clinical trials. Band-assisted ligation techniques, anti-reflux mucosectomy, anti-reflux mucosal ablation, and new plication devices have yielded promising results in recent noncontrolled studies. Nonetheless, the role of endoscopic procedures remains controversial due to limited long-term and comparative data, and no consensus exists in current clinical guidelines. This review provides an updated summary focused on the patient selection, technical details, clinical success, and safety of current and future endoscopic anti-reflux techniques.     

Primary pulmonary malignant melanoma diagnosed with percutaneous biopsy tissue: A case report

BACKGROUNDPrimary malignant melanoma of the lung (PMML) is a rare and highly malignant tumor with a poor prognosis. Here, we report a PMML case diagnosed by computed tomography (CT)-guided percutaneous biopsy, describe its pathological features and review relevant literature to improve our understanding of this tumor.CASE SUMMARYA 64-year-old Chinese female presented with productive cough for 7 mo. A chest CT scan showed a large and space-occupying lesion in Lingual lobe. Positron emission tomography-CT revealed multiple nodules located in the superior lobe apicoposterior segment of her left lung. Brain magnetic resonance imaging showed numerous enhancing nodules, suggesting brain metastasis. Abdominal CT scan did not show any abnormalities. By CT-guided percutaneous biopsy, four pieces of gray and taupe tissues (1 cm length and 0.1 mm in diameter) were obtained. After pathologic examination, the tumor was found to consist of epidermal and nested small round cells, fibrosis and thin-walled blood vessels. The finding was suggestive of malignant melanoma. To confirm the diagnosis, pathological morphology and immunophenotypic features of the biopsy specimens were observed. The patient denied any history of skin tumors. No abnormal lesions were detected in other sites of the body. Molecular testing was positive for wild-type EGFR and KIT gene mutations. Finally, the clinical and pathological findings suggested PMML. CONCLUSIONPMML is very rare, and the percutaneous biopsy tissue is limited. Therefore, comprehensive consideration of histology, immunohistochemistry, imaging, and clinical information is important for the diagnosis of PMML.     

蒙西医结合治疗慢性宫颈糜烂82例疗效观察

目的 :探讨蒙西药结合治疗宫颈糜烂。方法 :宫颈糜烂患者 82例 ,随机分为轻度、中度、重度。月经干净后第 3天开始隔日 1次上药 ,1 0次为 1个疗程 ,观察糜烂修复情况。结果 :第一疗程总有效率 70 .2 3% ;第二疗程总有效率 87.5 9% ;轻度糜烂疗效明显高于中度和重度。第二疗程后总有效率明显高于第一疗程。结论 :蒙药嘎木朱尔加用西药维生素 B2 和维生素 C片剂后 ,不但生肌收敛 ,还能促进局部血液循环 ,阻止上皮化生及癌变过程。     

Effects of β-Adrenergic Antagonists on the QT Measurements from Exercise Stress Tests in Pediatric Patients with Long QT Syndrome

It has been proposed that -adrenergic antagonist protection against cardiac events in patients with long QT syndrome (LQTS) may be related to a decrease in baseline QTc dispersion. To determine the effects of -blocker therapy on QT measurements, we evaluated the exercise tests of 25 pediatric patients with LQTS. Measurements were made of the maximum QTc interval and QTc dispersion during the various segments of the exercise test. There was no statistically significant difference between the pre--blocker and post--blocker maximum QTc interval during the supine (0.473 ± 0.039 vs 0.470 ± 0.038 sec), exercise (0.488 ± 0.044 vs 0.500 ± 0.026 sec), or recovery (0.490 ± 0.031 vs 0.493 ± 0.029 sec) phases of the exercise stress test. There was also no statistically significant difference between the pre--blocker and post--blocker QTc dispersion during the supine (0.047 ± 0.021 vs 0.058 ± 0.033 sec), exercise (0.063 ± 0.036 vs 0.063 ± 0.028 sec), or recovery (0.045 ± 0.023 vs 0.052 ± 0.026 sec) phases of the exercise stress test. Therefore, the protection that -blockers offer appears not to be related to a reduction of the baseline QTc interval or a decrease of QTc dispersion. Paper presented at the Third Annual World Congress of Pediatric Cardiology and Cardiac Surgery, Toronto, Canada, May 2001     

Rosiglitazone, a ligand of the peroxisome proliferator-activated receptor-gamma, reduces acute pancreatitis induced by cerulein

OBJECTIVE: In the present study, we investigated the effects of rosiglitazone (10 mg/kg, i.p.), a PPAR-gamma agonist, on the development of acute pancreatitis. DESIGN: Intraperitoneal injection of cerulein in mice induced an acute pancreatitis characterized by edema, neutrophil infiltration elevated serum levels of amylase and lipase. This experimental model was performed to test the anti-inflammatory activity of rosiglitazone. SETTING. University research laboratory. INTERVENTIONS: Male CD mice (20-22 g) were allocated into four groups (n=10 for each group): (a) Cerulein+vehicle group. Mice were treated hourly (x 5) with cerulein (50 microg/kg, in saline solution, i.p.); (b) Rosiglitazone group (same as the Cerulein+vehicle group but were administered rosiglitazone, 10 mg/kg bolus, 30 min prior to cerulein); (c) Sham+saline group. Mice were treated with saline instead of cerulein; (d) Sham+Rosiglitazone. Identical to Rosiglitazone group except that the saline was administered instead of cerulein. Mice were killed at 6 h after the induction of pancreatitis. Blood samples, pancreas, and lungs were collected. MEASUREMENTS AND RESULTS: Infiltration of pancreatic and lung tissue with neutrophils was associated with enhanced lipid peroxidation. Immunohistochemical examination demonstrated a marked increase in immunoreactivity for nitrotyrosine and for ICAM-1 in the pancreas of cerulein-treated mice. In contrast, the degree of (a) pancreatic inflammation and tissue injury, (b) upregulation/formation of ICAM-1 and nitrotyrosine, and (c) neutrophils infiltration was markedly reduced in pancreatic tissue obtained from rosiglitazone-treated mice. CONCLUSION: These findings support the view that rosiglitazone and other potent PPAR-gamma agonists may be useful in the therapy of acute pancreatitis.     

Rare case of fecal impaction caused by a fecalith originating in a large colonic diverticulum: A case report

BACKGROUNDFecal impaction is defined as a large mass of compacted feces in the colon and has the potential to induce a serious medical condition in elderly individuals. Fecal impaction is generally preventable, and early recognition of the typical radiological findings is important for making an early diagnosis. The factors that lead to fecal impaction are usually similar to those causing constipation. Few cases with fecal impaction associated with a diverticulum have been reported.CASE SUMMARYWe present the case of a 62-year-old woman who suffered from abdominal pain and vomiting, had a medical history of repeated acute abdomen and was diagnosed with fecal impaction in the descending colon based on X-ray and computed tomography (CT) imaging. After examination by gastrografin-enhanced colonography following colonoscopy and CT colonography, the fecalith was suspected to have been produced at the site of a large diverticulum in the transverse colon. The fecalith was surgically resected, and a histological diagnosis of pseudodiverticulum was made. There was no recurrence during 33 mo of follow-up.CONCLUSIONThis case highlights the importance of accurate identification and treatment of a fecal impaction. This case indicated that the endoscopic evacuation and subsequent colonography were effective for identifying a diverticulum that might have caused fecal impaction. A fecal impaction was associated with the diverticulum. Consequently, the planned diverticulectomy was performed. Appropriate emergency medical treatment and maintenance treatments should be selected in such cases to prevent recurrence.     

Alterations in gastric mucin synthesis by Helicobacter pylori

Helicobacter pylori is recognized as a cause of chronic active gastritis, gastric and duodenal ulcers, and gastric cancer, though the mechanisms of pathogenesis for H. pylori-associated diseases are not yet well understood[1 -4] The ecological niche to which H. pylori is well-adapted is the mucous layer of the human gastric antrum, which has mucin glycoproteins as major constituents. Mucins, highmolecular weight carbohydrate-rich glycoproteins that coat the surface of the stomach and are secreted into the lumen, function to protect the stomach and could be important in H. pylori colonization. For further understanding the pathogenesis of H. pylori-related diseases, it is important to consider whether H. pylori colonization of the surface epithelium is associated, as cause or effect, with changes in the gastric mucin synthesized by surface mucous cells.