Serum levels of the soluble low affinity receptor for IgE and soluble interleukin-2 receptor in childhood, and their relation to age, gender, atopy and allergic disease

Identifying immune factors associated with the development of atopy can enhance our understanding of the in vivo mechanisms involved and may have utility in paradigms designed to prevent disease. Two candidates sug-gested for such roles are the soluble low affinity receptor for IgE (sCD23) and the soluble interleukin-2 receptor (sCD25). To assess serum levels of these factors blood samples were collected at birth and at age 6 in a large nonselected population from Tucson, AZ. Log mean sCD23 and sCD25 lev-els decreased from birth to age 6, (for sCD23 0. 60 ffi 0. 26pg/l, n=340 and 0. 53 + 0. 28pg/l, n=333 and for sCD25 1. 95 i 0. 14pM. n=304 and 1. 86 ffi 0. 20pM, n=327. for the two ages respectively. Anglo children had lower sCD23 levels at birth compared to Hispanic children (p < 0.01); no effect of gender was observed. Skin test reactivity at age 6 was directly related to sCD25 levels at age 6 (p=0.007) and even levels at birth showed a similar trend (p=0.06). These relations were distinct from any relation to total se-rum IgE. No relation was observed with sCD23 levels for either skin test re-activity or serum IgE. The prevalences of asthma, rhinitis and eczema by age 6 were unrelated to sCD25 or sCD23 levels. The results indicate that soluble CD23 and CD25 have higher levels at birth than later in childhood and that the development of skin test reactivity may be associated with reg-ulatory mechanisms involving sCD25, whereas sCD23 was not similarly implicated.     

Antiallergic action of Magnolia officinalis on immediate hypersensitivity reaction

We studied the effect of aqueous extract of Magnolia officinalis bark (Magnoliaceae) (MOAE) on the immediate hypersensitivity reaction. MOAE (0.01 to 1 g/kg) dose-dependently inhibited compound 48/80 induced systemic anaphylaxis in rats. MOAE (0.1 and 1 g/kg) also significantly inhibited local immunoglobulin E (IgE)-mediated passive cutaneous anaphylactic reaction. When MOAE was pretreated at concentrations ranging from 0.01 to 1 g/kg, the levels of plasma histamine were reduced in a dose-dependent manner. MOAE (0.001 to 1 mg/ml) dose-dependently inhibited the histamine release from rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-dinitrophenyl (DNP) IgE. The level of cyclic AMP (cAMP) in RPMC, when MOAE was added, significantly increased compared with that of the normal control. Moreover, MOAE (0.01 to 1 mg/ml) had a significant inhibitory effect on anti-DNP IgE-induced tumor necrosis factor-alpha production from RPMC. These results indicate that MOAE inhibits immediate hypersensitivity reaction in vivo and in vitro.     

鼻渊灵穴位敷贴对儿童变应性鼻炎血清IgE、ICAM-1及VCAM-1的影响

目的：观察鼻渊灵穴位敷贴对儿童变应性鼻炎血清Ig E、ICAM-1及VCAM-1的影响。方法：选择变应性鼻炎患儿96例,随机分为治疗组和对照组各48例。治疗组给予鼻渊灵穴位敷贴治疗,对照组给予粉尘螨滴剂治疗。结果：两组Ig E和ICAM-1、VCAM-1水平与治疗前比较,有统计学意义（P〈0.05）,两组间比较无显著性差异（P〉0.05）;两组总有效率比较,治疗组优于对照组（P〈0.05）。结论：鼻渊灵穴位敷贴能降低变应性鼻炎患儿血清Ig E、ICAM-1及VCAM-1水平,改善患儿临床症状。     

Association between the MB system and asthma in the Japanese

Twenty-three unrelated Japanese patients with asthma who showed a high total serum IgE level, a strong skin test response to Dermatophagoides farinae, and a high score on a radioallergosorbent test (RAST) using Dermatophagoides farinae were typed for HLA-A locus, -B locus, and -D region antigens. No significant difference in the frequencies of HLA-A, -B, and -DR antigens were observed between the asthma patients and the healthy controls. A significant difference in the frequency of MB3', however, was found between the asthma patients and the healthy controls (corrected p = 0.04, relative risk = 18.5).     

Immediate contact hypersensitivity induced by repeated hapten challenge in mice

An immediate reaction was investigated during repeated challenge testing for contact hypersensitivity to dinitrofluorobenzene (DNFB) in BALB/c mice. The mice were sensitized to DNFB on back skin and repeatedly challenged with the same hapten on the left ear at 1 week intervals. The ear after the 5th challenge showed biphasic responses which consisted of an immediate and a delayed-type reaction. The reactions were hapten specific. Mast cell-deficient WBB6F1 W/WV mice did not show any immediate reaction, while congenic normal mice showed both immediate and delayed-type reactions. Histologically, numerous dermal mast cells were found in the left ear of repeatedly challenged BALB/c and WBB6F1 normal mice, while there were few mast cells in the ear of WBB6F1 W/WV mice. Anti-DNP IgE antibodies were detected in BALB/c, WBB6F1 normal and W/WV mice after repeated challenge with DNFB. Intradermal injection of anti-IgE antibodies in the repeatedly DNFB-challenged ear elicited an immediate reaction. These results suggest that immediate contact hypersensitivity develops through the production of anti-DNP IgE antibodies and an increase in dermal mast cells after repeated challenge with DNFB.     

针刺结合自血穴注治疗变应性鼻炎60例

目的探索一种治疗变应性鼻炎的方法。方法60例变应性鼻炎患者随机分为两组。观察组用针刺结合自血穴注疗法，对照组用针刺结合药物穴注疗法。同时，分别于治疗前后进行血清免疫球蛋白（IgE）检测。结果观察组总有效率为83.33％对照组总有效率为76．67％，观察组明显高于对照组（P〈0．05）。治疗前后两组血清免疫球蛋白（IgE）检测改变均有显著性差异（P〈0.05）；治疗前后两组血清免疫球蛋白（IgE）差值之差异均有显著性意义（P〈0．05）。结论针刺结合自血穴注疗法对于变应性鼻炎患者具有确切疗效其治疗机理与免疫调节相关。     

血清总IgE在儿童呼吸系统疾病诊疗中的应用价值

目的 初步探讨血清总IgE在儿童呼吸系统疾病诊疗中的应用价值.方法 以30例正常儿童为对照,回顾性分析125例哮喘、支气管肺炎、毛细支气管炎、支气管炎4组呼吸系统疾病患儿血清的总IgE.结果 哮喘与支气管肺炎组IgE水平均高于正常对照组,差异有统计学意义（P＜0.05）;毛细支气管炎组和支气管炎组与正常对照比较差异无统计学意义（P ＞0.05）;125例患儿血清总IgE阳性率为64.8％,其中哮喘组阳性率100％,其总IgE水平高于其他疾病组,差异有统计学意义（P＜0.05）.结论 血清总IgE检测可辅助诊断儿童呼吸系统疾病,疾病早期检测血清总I班可有效减低哮喘的发生.     

鸡蛋过敏患儿蛋清4种组分slgE抗体分布特征*

摘要:目的 分析鸡蛋过敏患儿血清中蛋清4种过敏原蛋白组分的slgE,抗体分布特征,明确鸡蛋过敏单组分解析诊断的临床应用价值。方法﹐收集197例鸡蛋过敏儿童血清样本,采用光激化学发光法检测蛋清中4种主要过敏原组分蛋白(卵类黏蛋白卵白蛋白卵转铁蛋白溶菌酶)sIgE抗体的水平,并分析不同组分slgE抗体的分布特征。结果152例(77.16%)患儿血清中含有卵白蛋白slgE抗体,139例(70.56%)含有卵类黏蛋白slgE,抗体,69例(35.02%)含有卵转铁蛋白slgE抗体,但仅有36例(18.27%)患儿血清中含有溶菌酶sIgE.,抗体。30.45%的患儿血清中同时含有针对卵类黏蛋白和卵白蛋白的sIgE抗体;鸡蛋sIgE抗体含量与蛋清4种组分slgE抗体累计含量的相关性不显著(r=0.266 8,P<0.05) ,且不同患儿血清中蛋清4种组分sIgE抗体的含量存在明显的个体差异。结论―患儿血清中蛋清4种组分slgE抗体含量存在个体间异质性,蛋清组分slgE抗体检测可以区分不同形式的鸡蛋过敏,对于儿童鸡蛋过敏的精准诊断与精准脱敏治疗具有重要的价值。     

The Phadiatop test allows adequate screening for atopy with a marked reduction in cost

Sera from 35 patients aged 5 years or younger and sera from 95 patients 6 years and older were assayed for both total IgE level, allergen-specific IgE by RAST and either the Phadiatop Paediatric or Phadiatop procedures, depending on age. Good agreement was found allowing exclusion of specimens anticipated to be RAST negative with an expected reduction in RAST testing of up to 80% of the present workload, without significant exclusion of RAST positive specimens.