复合氨基酸胶囊中水溶性维生素的含量测定

目的:建立复合氨基酸胶囊中5种水溶性维生素的含量测定方法.方法:采用高效液相色谱法,用乙腈与庚烷磺酸钠-磷酸钠的水溶液(pH=3.0)为流动相,流速1.0ml·min-1,色谱柱为C18(4.6×250mm,5μm),柱温40℃,检测波长为280nm,进样量为20μl.结果:维生素Bl、维生素B2、维生素B6、维生素C和烟酰胺分别在24.7～75.2μg·ml-1、15.0～44.9μg·ml-1、12.2～37.5μg·ml-1、99.2～299.9μg·ml-1和100.3～300.9μg·ml-1范围内呈线性关系,相关系数均为1.000,平均回收率均在100%±2%范围以内.RSD分别为0.19%、0.26%、0.46%、1.17%和0.52%.结论:本方法准确,简便,快捷,可以同时测定复合氨基酸胶囊中维生素B1、维生素B2、维生素B6、维生素C和烟酰胺的含量.     

四川市售白芍饮片质量调研

目的:调研四川市售白芍饮片质量。方法:随机采购四川市售白芍饮片16批,参照现行2010版药典的规定,测定饮片水分、总灰分、水溶性浸出物,并采用HPLC法测定芍药苷含量。结果:16批次白芍饮片中仅3批次芍药苷的量符合药典规定,9批次水分符合药典规定。结论:白芍饮片存在的质量问题主要是芍药苷含量和水分含量不合格。针对导致白芍药材或饮片质量问题的多种原因,药品监督管理部门应加强监督管理,规范饮片市场。     

Proposal of a flow scheme for the chemical-form-based quantitative analysis of chlorine compounds in pulp for sanitary products and verification of safety

To determine the amounts and chemical forms of chlorine compounds in elemental chlorine-free (ECF) bleached pulp for sanitary products, a chemical-form-based quantitative analysis flow scheme was created. The scheme involves quantitative determination of compounds eluted in elution tests assuming dermal exposure. The results indicated that most of the chlorine compounds in pulp used for sanitary products were insoluble organically bound chlorine and water-soluble chlorides consisting of chloride ions. The total amount of chlorine obtained by analysis of the total chlorine in the pulp products was close to the sum of the amounts of water-soluble chlorides, residual chlorine, chloroacetic acids, and insoluble organically bound chlorine. Therefore, the balance of chlorine measured by the flow scheme was acceptable. Little residual chlorine was detected in the pulp products, suggesting that the chlorine dioxide used for bleaching was almost completely converted into inactive inorganic chlorides and organic chlorine compounds and that these substances do not affect the health risk posed by pulp products. A risk assessment of the detected chloroacetic acid and dichloroacetic acid revealed that there is no concern about the health risk posed by use of these products.     

Distribution of ochratoxin A in plasma and tissues of rats fed a naturally contaminated diet amended with micronized wheat fibres: effectiveness of mycotoxin sequestering activity.

The effectiveness of micronized wheat fibres (MWF) alone or in association with yeast cell walls (YCW) as active adsorbents to decrease, in vivo, the levels of ochratoxin A (OTA) was checked in a total of 48 rats, equitably distributed into four groups: (1) control; (2) OTA naturally contaminated diet (2.2 microg/g); (3) OTA naturally contaminated diet (2.2 microg/g) amended with MWF (2%); (4) OTA naturally contaminated diet (2 microg/g) amended with MWF (1.8%) in association with YCW (0.2%). A 4 week experimental period corresponding to a daily intake in the range of 132.2-146.1 microg OTA/kg bw decreased the rat body weight gains, as compared to the controls. The adsorbents did not significantly alleviate the growth depression caused by the contaminated diet. However, a significant protective effect of MWF was observed in terms of OTA concentration in plasma (40.5% decrease), kidney (28.1% decrease) and liver (38.8% decrease). Mixing this sorbent with the YCW did not significantly improve its protective activity against OTA. The faecal OTA concentrations were higher for the MWF and MWF+YCW treated animals, as compared to the positive control (group II). Taken together, these results suggest that MWF are a promising tool to counteract the toxic effects of OTA naturally contaminated diets.     

制何首乌质量标准的初步研究

用偏光显微镜观察了淀粉粒在何首乌炮制过程中的变化，并结合测定水溶性浸出物含量，能有效地控制何首乌炮制质量。     

肝毒清方单味药水提物的体外抗HBV作用

采用 2.2 15细胞为靶细胞,Ara-Amp为阳性对照药,观察肝毒清方单味药不同浓度水提物对2.2.15细胞分泌HBsAg、HBeAg的影响。结果表明,白马骨、木芙蓉、毛鸡屎藤、白花丹、山竹子、秋海棠水提物在一定浓度范围内,对2.2.15细胞分泌HBsAg、HBeAg有抑制作用。     

Toll-like receptor-2 agonist functionalized biopolymer for mucosal vaccination

The objective of this study was to provide a new water-soluble chitosan derivative being functionalized with a Toll-like receptor-2 (TLR-2) agonist. At first, we synthesized the water-soluble TLR-2 agonist ω-amido-[N_α-palmitoyl-oxy-S-[2,3-bis(palmitoyl-oxy)-(2R)-propyl]-[R]–cysteinyl]-α-amino poly(ethylene glycol) (Pam₃Cys-PEG-NH₂), which was characterized by ¹H and ¹³C NMR as well as mass spectroscopy. Secondly, Pam₃Cys-PEG-NH₂ was then successfully grafted to 6-O-carboxymethyl-N,N,N-trimethyl chitosan polymers (CM-TMC) using EDC/NHS as condensing agents. The copolymer was analysed by means of ¹H and ¹³C NMR and FTIR spectroscopy. ¹³C NMR spectroscopy did not deliver evidence that an amide bond was formed between CM-TMC and Pam₃Cys-PEG-NH₂. However, ¹H NMR and FTIR spectroscopy demonstrated clearly that successful grafting took place. Based upon these results, this new TLR-2 functionalized biopolymer merits further investigations as material for vaccine delivery systems.     

内蒙古西部地区土壤中水溶性硒含量分布研究

目的：研究内蒙古西部地区土壤中水溶性硒含量分布,为富硒作物种植地区的选择提供科学依据。方法：采集579份耕地土壤样品,用流动注射氢化物发生原子吸收光谱法测定其中的水溶性硒含量。结果：巴彦淖尔、包头、鄂尔多斯、乌海4个地区地下水灌溉的土壤中水溶性硒含量差异有统计学意义,其中巴彦淖尔、包头地区地下水灌溉的土壤中水溶性硒含量均高于鄂尔多斯;巴彦淖尔、包头地下水与黄河水灌溉的土壤中水溶性硒含量差异均有统计学意义,均为地下水灌溉的土壤中水溶性硒含量值较高;巴彦淖尔和包头两地黄河水灌溉的土壤中水溶性硒含量差异无统计学意义。结论：在被调查的四个地区中,巴彦淖尔和包头地区地下水灌溉的土壤中水溶性硒含量最高,可考虑作为富硒作物的种植地区。     

Fluorescent tracking of nickel ions in human cultured cells

The carcinogenic activity of various nickel (Ni) compounds is likely dependent upon their ability to enter cells and elevate intracellular levels of Ni ions. Water-insoluble Ni compounds such as NiS and Ni(3)S(2) were shown in vitro to enter cells by phagocytosis and potently induce tumors in experimental animals at the site of exposure. These water-insoluble nickel compounds are generally considered to be more potent carcinogens than the water-soluble forms. However, recent in vitro studies have shown similar effects for insoluble and soluble Ni compounds. Using a dye that fluoresces when intracellular Ni ion binds to it, we showed that both soluble and insoluble Ni compounds were able to elevate the levels of Ni ions in the cytoplasmic and nuclear compartments. However, when the source of Ni ions was removed from the culture dish, the intracellular Ni ions derived from soluble Ni compound were lost from the cells at a significantly faster rate than those derived from the insoluble Ni compound. Within 10 h after NiCl(2) removal from the culture medium, Ni ions disappeared from the nucleus and were not detected in the cells by 16 h, while insoluble Ni(3)S(2) yielded Ni ions that persisted in the nucleus after 16 h and were detected in the cytoplasm even after 24 h following Ni removal. These effects are discussed in terms of whole body exposure to water-soluble and -insoluble Ni compounds and consistency with animal carcinogenicity studies.     

Reversed-phase ion-pair HPLC determination of some water-soluble vitamins in pharmaceuticals

A reversed-phase ion-pair high-performance liquid chromatografic method (RP-IPC) was developed to assay some water-soluble vitamins in solution dosage forms. Vitamins of the B-group B₁, B₂, B₃, and B₆, including vitamin C were determined in Oligovit^® coated tablets. In Beviplex^® coated tablets the vitamins B₁, B₂, B₃, B₆ and p-aminobenzoic acid were analysed. Hexanesulphonic acid sodium salt and triethanolamine in water–methanol were used as mobile phase with adjusting pH to 2.8 with orthophosphoric acid. Phenol was used as an internal standard. For quantitative simultaneous analysis of vitamins in pharmaceutical formulations, the method of internal standard was used. All parameters for the validation of the method are given.