盐酸乌苏里藜芦碱对麻醉犬血流动力学的研究

 目的:探讨辽宁千山产的乌苏里藜芦生物碱对麻醉犬的多项血流动力学参数的影响。方法:用MFV - 1200型电磁流圣计及RM-6000型八道生理记录仪分别测定对照组及Vera-U(iv 1 μg·kg^-1)和Vera-U(iv 3 μg·kg^-1)组麻醉犬的多项血流动力学参数。结果:1 μg·kg^-1,3 μg·kg^-1的盐酸乌苏里藜芦碱可使受试动物的血压、左室内压、左室内压变化速率、左室作功指数、心肌耗氧指数发生显著降低(P<0.01)，使CO明显减少(P<0.05).结论:实脸结果提示，盐酸藜芦碱的降压作用主要是通过扩张血管、降低心脏负荷来实现的，是否有中枢性降压机制的参与还有待探讨。     

毛穗藜芦中茋类化合物的化学研究

目的：对毛穗藜芦根茎进行化学成分研究。方法：采用柱层析和薄层层析法进行分离，用ＵＶ，ＩＲ，ＭＳ，Ｈ-ＮＭＲ等光谱技术鉴定化合物的结构。结果：得到２个类化合物，为白藜芦醇和２，３′，４，５′-四羟基。结论：为首次从该植物中分离得到。     

毛穗藜芦中茋类化合物的化学研究

目的：对毛穗藜芦根茎进行化学成分研究。方法：采用柱层析和薄层层析法进行分离，用ＵＶ，ＩＲ，ＭＳ，１ＨＮＭＲ等光谱技术鉴定化合物的结构。结果：得到２个类化合物，为白藜芦醇和２，３′，４，５′四羟基。结论：为首次从该植物中分离得到     

苦参碱对人增生性瘢痕成纤维细胞增殖的体外抑制作用

组织损伤修复是人体自我保护的一个正常病理过程,而过度修复则会出现组织纤维化,影响组织、器官的正常功能,人增生性瘢痕是创伤过度修复的异常结果。苦参碱(matrine,Mat)广泛存在于豆科植物苦参、苦豆子及广豆根中,近年研究发现其具有较强的抗组织纤维化作用[1],目前关于Mat对人增生性瘢痕成纤维细胞(下文简称为成纤维细胞)作用的报道较少。本实验以成纤维细胞为靶细胞观察不同质量浓度的Mat在体外对该细胞增殖的影响,并对其抗纤维化作用机制进行初步研究。1材料与仪器苦参碱标准品(中国药品生物制品检定所提供);DMEM培养基(Gibco公司产…     

龙葵全草皂苷类化学成分研究

目的研究龙葵Solanum nigrum全草的皂苷类化学成分。方法采用硅胶、反相ODS开放柱色谱及反相HPLC等手段分离化合物,运用波谱技术分析确定化学结构。结果龙葵全草60%乙醇提取物经D-101大孔树脂柱吸附,获得的60%乙醇洗脱部分再经分离得到8个化合物。利用理化及波谱分析确定这些化合物结构分别为uttroside B(Ⅰ)、uttroside A(Ⅱ)、22α,25R-26-O-β-D-吡喃葡萄糖基-22-羟基-呋甾-Δ5-3β,26-二醇-3-O-β-D-吡喃葡萄糖基-(1→2)-O-[β-D-吡喃木糖基-(1→3)]-O-β-D-吡喃葡萄糖基-(1→4)-O-β-D-吡喃半乳糖苷(Ⅲ)、22α,25R-26-O-β-D-吡喃葡萄糖基-22-甲氧基-呋甾-Δ5-3β,26-二醇-3-O-β-D-吡喃葡萄糖基-(1→2)-O-[β-D-吡喃木糖基-(1→3)]-O-β-D-吡喃葡萄糖基-(1→4)-O-β-D-吡喃半乳糖苷(Ⅳ)、5α,22α,25R-26-O-β-D-吡喃葡萄糖基-22-羟基-呋甾-3β,26-二醇-3-O-β-D-吡喃葡萄糖基-(1→2)-O-[β-D-吡喃葡萄糖基-(1→3)]-O-β-D-吡喃葡萄糖基-(1→4)-O-β-D-吡喃半乳糖苷(Ⅴ)、5α,22α,25R-26-O-β-D-吡喃葡萄糖基-22-甲氧基-呋甾-3β,26-二醇-3-O-β-D-吡喃葡萄糖基-(1→2)-O-[β-D-吡喃葡萄糖基-(1→3)]-O-β-D-吡喃葡萄糖基-(1→4)-O-β-D-吡喃半乳糖苷(Ⅵ)、dumoside(Ⅶ)、5α,20S-3β,16β-二醇-孕甾-22-羧酸-(22,16)-内酯-3-O-β-D-吡喃葡萄糖基-(1→2)-O-[β-D-吡喃木糖基-(1→3)]-O-β-D-吡喃葡萄糖基-(1→4)-O-β-D-吡喃半乳糖苷(Ⅷ)。结论化合物Ⅲ～Ⅷ为首次从该属植物中分离得到。     

岩高兰生药学研究

目的对药用植物岩高兰的原植物形态、药材性状进行鉴别研究,为开发利用岩高兰提供依据.方法性状鉴别、显微鉴别、化学系统预示和薄层色谱相结合.结果对生药性状、显微特征和化学系统预示试验,薄层色谱实验进行观察描述.结论此研究结果可为挖掘中药新资源,进一步保护和开发利用岩高兰提供依据.     

γ-亚麻酸乙酯的合成研究

目的确定合成γ 亚麻酸乙酯的最佳工艺路线。方法以黑加仑油为原料采用尿素包和、柱色谱法、酸催化法等联合方法分离及合成γ 亚麻酸乙酯。结果确定黑加仑油经 2～ 3次尿素包和 ,反应温度 80℃ ,反应时间90min ,3%盐酸作酸性溶媒 ,乙醇和γ 亚麻酸比例为 3∶1的制备工艺。结论酸催化法合成γ 亚麻酸乙酯工艺简便 ,易于操作 ,产品收率高、稳定性好。     

白藜芦醇对视网膜母细胞瘤细胞多药耐药性相关因子表达的影响

目的 观察白藜芦醇对视网膜母细胞瘤(RB)细胞多药耐药性(MDR)因子表达的影响.方法 体外培养RB细胞,取对数生长期细胞进行实验.实验分为实验组及对照组进行.采用浓度为6.25、12.50、25.00、50.00、100.00 μmol/L的白藜芦醇处理RB细胞,作用24、48 h后,噻唑蓝(MTT)比色法检测不同浓度组RB细胞的吸光度[A,旧称光密度(OD)]值,以此表示RB细胞活性.采用浓度为50.00 μmol/L的白藜芦醇处理RB细胞48 h,逆转录聚合酶链反应(RT-PCR)和蛋白质免疫印迹法(Western blot)分别检测MDR-1、环氧化酶(COX)-2、多药耐药相关蛋白(MRP)-1、谷胱苷肽转移酶(GST)-π的m RNA和蛋白表达.3种检测均以加入等体积0.5％二甲基亚砜细胞培养液为对照组.结果 MTT比色法检测结果显示,与对照组比较,6.25、12.50、25.00、50.00 μmol/L实验组A值呈剂量依赖性降低,差异有统计学意义(F=4.782,P＜0.05).50.00、100.00 μmol/L实验组A值间差异无统计学意义(F=6.351,P＞0.05).RT-PCR检测结果显示,实验组MDR-1、MRP1、COX-2、GST-π mRNA表达较对照组显著降低,差异均有统计学意义(t=9.170、5.758、4.152、4.638,P＜0.05).Western blot检测结果显示,实验组MDR-1、MRP1、COX-2、GST-π蛋白表达较对照组显著降低,差异均有统计学意义(t=3.848、5.955、4.541、3.514,P＜0.05).结论 白藜芦醇可降低RB细胞MDR因子的表达.     

Mistaken identity: Severe vomiting,bradycardia and hypotension after eating a wild herb

A 53-year-old woman presented with severe vomiting 3.5 hours after eating a boiled wild herb thought to be Hosta montana. She was alert, and did not complain of diplopia or numbness of her limbs. Her vital signs were temperature of 35.9°C, blood pressure of 84/33 mmHg, and heart rate of 59 beats/minute. There was no arrhythmia or conduction block on electrocardiogram. Her blood pressure and heart rate were decreased to 75/44 mmHg and 51 beats/minute at the minimum, respectively. Complete blood count and serum chemistry were normal. The herb was correctly identified when the patient's husband brought in the herb for identification.     

Integrated plant nutrient system – with special emphasis on mineral nutriton and biofertilizers for Black pepper and cardamom – A review

Abstract

Integrated Plant Nutrition System (IPNS) as a concept and farm management strategy embraces and transcends from single season crop fertilization efforts to planning and management of plant nutrients in crop rotations and farming systems on a long-term basis for enhanced productivity, profitability and sustainability. It is estimated that about two-thirds of the required increase in crop production in developing countries will have to come from yield increases from lands already under cultivation. IPNS enhances soil productivity through a balanced use of soil nutrients, chemical fertilizers, combined with organic sources of plant nutrients, including bio-inoculants and nutrient transfer through agro-forestry systems and has adaptation to farming systems in both irrigated and rainfed agriculture. Horticultural crops, mainly plantation crops, management practices include application of fertilizers and pesticides which become inevitable due to the depletion of soil organic matter and incidence of pests and diseases. The extensive use of chemical fertilizers in these crops deteriorated soil health that in turn affected the productivity. To revitalize soil health and to enhance productivity, it is inexorable to enrich the soil using microorganisms. The lacunae observed here is the lack of exploitation of indigenous microbes having the potential to fix atmospheric nitrogen (N) and to solubilize Phosphorus (P) and Potassium (K). The concept of biofertilizer application appears to be technically simple and financially feasible, but the task of developing biofertilizers with efficient strains in appropriate combinations in a consortia mode is not easier. More than developing consortia, a suitable delivery system to discharge the microbial inoculants warranted much effort. This review focuses on the integrated plant nutrition system incorporating biofertilizer with special emphasis on developing and formulating biofertilizer consortium.