糙叶败酱活性成分的研究

采用硅胶柱层析技术从中药糙叶败酱(Patrinia scabra Bunge)的根中分离得到了5个化合物,经理化常数测定和波谱分析,鉴定其分别为落叶松脂醇(Ⅰ)、丁香树脂醇(Ⅱ)、东莨菪内酯(Ⅲ)、槲皮素(Ⅳ)、阿魏酸(Ⅴ)。所有化合物均首次从该植物中分得。     

Syringaresinol protects against hypoxia/reoxygenation-induced cardiomyocytes injury and death by destabilization of HIF-1α in a FOXO3-dependent mechanism

Hypoxia-inducible factor 1 (HIF-1) is a master regulator of hypoxic response and has been a prime therapeutic target for ischemia/reperfusion (I/R)-derived myocardial dysfunction and tissue damage. There is also increasing evidence that HIF-1 plays a central role in regulating aging, both through interactions with key longevity factors including Sirtuins and mTOR, as well as by directly promoting longevity in Caenorhabditis elegans. We investigated a novel function and the underlying mechanism of syringaresinol, a lignan compound, in modulation of HIF-1 and protection against cellular damage and death in a cardiomyocyte model of I/R injury. Syringaresinol caused destabilization of HIF-1α following H/R and then protected against hypoxia/reoxygenation (H/R)-induced cellular damage, apoptosis, and mitochondrial dysfunction in a dose-dependent manner. Knock-down of FOXO3 by specific siRNAs completely abolished the ability of syringaresinol to inhibit HIF-1 stabilization and apoptosis caused by H/R. Syringaresinol stimulated the nuclear localization and activity of FOXO3 leading to increased expression of antioxidant genes and decreased levels of reactive oxygen species (ROS) following H/R. Our results provide a new mechanistic insight into a functional role of syringaresinol against H/R-induced cardiomyocyte injury and death. The degradation of HIF-1α through activation of FOXO3 is a potential therapeutic strategy for ischemia-related diseases.     

Lignans and neolignans from Stelleropsis antoninae

BACKGROUND AND THE PURPOSE OF THE STUDY: Stelleropsis antoninae Pobed. (Family: Thymelaeaceae) grows wildly as an herbaceous plant in Iran. Most of the Thymelaeaceous plants contain lignans and neolignans, which have important pharmacologically properties. In the present study, the isolation and identification of the main lignans and neolignans of S. antoninae, which has not been previously reported is described and compared to other species. METHODS: Column (CC) and High Performance Liquid Chromatographic (HPLC) methods were used for the isolation and purification, and (1)H-NMR, (13)C-NMR, HMBC, HMQC, H-H COSY and MS were employed for the identification of the compounds isolated from the methanol extract. RESULTS: From the methanol extract of the aerial parts of S. antoninae four lignans, syringaresinol (1), syringaresinol 4-O-β-D-glucopyranoside (4), syringaresinol 4-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside (5), liriodendrin (6), and two neolignans, 5-methoxylariciresinol 4'-O- β-D-glucopyranoside (3) and dehydrodiconiferyl alcohol 4-O-β -D-glucopyranoside (2) were isolated and identified. MAJOR CONCLUSION: The results of this study show that siringaresinol, a well-known bioavtive compound, and its glucosides are the main lignans, and lariciresinol and coniferyl alcohol derivatives are the main neolignans of S. antoninae.     

A new pterocarpan, (-)-maackiain sulfate,from the roots of<Emphasis Type="Italic">Sophora subprostrata</Emphasis>

A new pterocarpan, (-)-maackiain 3-sulfate (1) was isolated from the methanol extract of roots of Sophora subprostarata together with (-)-maackiain (2), trifolirhizin (3), lupeol (4), ononin (5), 7,4'-dihydroxyflavone (6), and (+)-syringaresinol (7). The structure of 1 was determined by analyses of 2D NMR and HRFABMS. Compounds 5-7 were isolated from this plant for the first time.     

Lignanoids from Solanum lyratum

In the present study, we aimed to investigate the chemical constituents from Solanum lyratum.The constituents were separated by column chromatography on silica gel, Sephadex LH-20 and preparative HPLC. Their structures were elucidated by spectroscopic means. A total of 13 compounds were isolated fromS. lyratumand identified as syringin (1), (+)-isolariciresinol (2), (+)-syringaresinol (3), leptolepisol D (4),(–)-secoisolariciresinol (5), (–)-epi-syringaresinol (6),aviculin(7), zhebeiresinol(8), ciwujiatone(9),(–)-(7′S,8S,8′R)-4,4′-dihydroxy-3,3′,5,5′-tetramethoxy-7′,9-epoxylignan-9′-ol-7-one(10),(+)-lariciresinol(11), (+)-pinoresinol (12) and (+)-medioresinol (13). All the compounds were isolated from S. lyratumfor the first time.