激光上皮下角膜磨镶术后角膜上皮瓣临床观察

目的 观察及探讨准分子激光上皮下角膜磨镶术(Laser subepithel ialkeratomileusis，LASEK)后，角膜上皮瓣的成活率及其影响因素。方法 对行LASEK治疗的42例(80眼)于术后1、2、3天，1、2、3、4周在裂隙灯显微镜下进行角膜上皮瓣的观察。结果 34例(68眼)，角膜上皮瓣成活，成活占85％(68／80)；未成活8例(12眼)，未成活占15％(12／80)。结论 LASEK术后角膜上皮瓣成活率的高低，决定着LASEK的临床疗效，影响其成活的因素是多方面的。其中角膜上皮瓣边缘不整齐、破裂、对位不良、操作时间过长可能是其主要原因。     

Survivin在过氧化氢预处理诱导的适应性细胞保护中的作用

目的探讨H2O2预处理对存活素(survivin)表达的影响及存活素在H2O2预处理的适应性细胞保护中的作用。方法在PC12细胞建立H2O2预处理对抗H2O2诱导细胞凋亡的实验模型,应用甲氮甲唑蓝(MTT)法检测细胞存活率,碘化丙啶(PI)染色流式细胞术检测细胞凋亡率,Hoechst染色检测细胞凋亡的形态学改变,免疫印迹法(Westernblot)测定存活素的表达水平。结果用100μmol/LH2O2预处理PC12细胞90min可明显地抑制300μmol/LH2O2作用12h后引起的细胞毒性和细胞凋亡,并可以显著地上调PC12细胞存活素的表达;JAK2抑制剂AG-490不仅可以抑制存活素的表达,而且拮抗H2O2预处理的适应性细胞保护作用。结论存活素是JAK-STAT通路的靶基因,可能在H2O2预处理诱导的适应性细胞保护机制中起着重要的作用。     

An infant with thanatophoric dwarfism surviving 169 days

A female infant with thanatophoric dwarfism surviving 169 days is described. The genetics of the entity are briefly discussed.     

Orthopaedic patients' experience of motor vehicle accident in Singapore

Aim:  The purpose of this paper is to present a study that explored the experiences of orthopaedic patients injured in motor vehicle accidents (MVAs), from the time of the accident until 6 months after being discharged from hospital.
Background:  Trauma injuries from MVAs are increasing, with the number of deaths from such injuries continuing to rise. Victims often sustain open fractures to more than one part of their body and need rehabilitation and support to adjust to long-term chronic or permanent disability. In the last decade, research pertaining to trauma nursing has concentrated on neurologically injured patients. Although there is a paucity of research on the nursing perspective of psychological care for non-neurologically injured patients, the majority of studies located were mainly quantitative in nature and did not analyse the personal experiences of orthopaedic patients.
Method:  A qualitative naturalistic inquiry approach was used, which provided a first-hand account of the traumatic MVA event experienced by six orthopaedic participants in Singapore. Data were collected from face-to-face in-depth interviews. Participants were voluntarily recruited through purposeful sampling and 'snowballing'. Interviews were audiotaped and transcribed verbatim in preparation for analysis.
Findings:  The analysis of information explicated four main themes: the experience of the event, the effect of hospitalization, surviving the event and self-transformation.
Conclusion:  The study provided an understanding of orthopaedic patients' experience of MVA in Singapore. The findings of the study have the potential to contribute to the limited qualitative research available concerning victims' experiences of MVAs and nurses caring for MVA victims.     

福建省残存嗜人按蚊清除效果观察

目的：清除残存嗜人按蚊,阻断疾病传播。方法：搜索残存嗜人按蚊,实施溴氰菊酯浸泡蚊帐灭蚊,复查所有嗜人按蚊分布点,评价清除效果。结果：1989-1994年,于建阳、武夷山、邵武、顺昌和三元等县（市、区）发现残存嗜人按蚊分布点42个,嗜人按蚊占人房按蚊组成的26.20%。2001年完成闽北地区灭蚊后全部嗜人按蚊分布点的复查工作,95.80%分布点经2-3次以上反复性复查,最多达12次,所有复查点均未再捕获嗜人按景。1988年清除工作前,分布区疟疾发病1381例,年发病率为4.47/万。采取措施后,疟疾发病率显著下降,1995年后,嗜人按蚊分布区未再发生疟疾暴发流行。1998-2001年,未发现当地疟疾感染者。结论：闽北地区嗜人按蚊巳被清除,当地疟疾传播得到有效阻断。