HPLC法测定滑膜炎颗粒中原儿茶醛的含量

目的建立滑膜炎颗粒剂中原儿茶醛的含量测定方法质量。方法采用高效液相色谱法对方中原儿茶醛进行含量测定。结果高效液相色谱法测定结果表明原儿茶醛在0．1005～1．005μg范围内呈线性关系，平均加样回收率为99．22％；RSD为0．72％。结论本法简便，重现性好、结果可靠，可作为控制滑膜炎颗粒剂的质量方法。     

正交优选法筛选荆芥炭炮制的最佳工艺

目的　筛选荆芥炭炮制的最佳工艺。方法　采用分光光度法,测定不同工艺炮制的荆芥炭中总黄酮的含量。结果　荆芥炭炮制的最佳条件是2 10℃、加热10 m in。结论　重复试验表明,所选的最佳炮制工艺较为合理。     

河南不同产地夏枯草中迷迭香酸的对比分析

目的：测定河南不同产地夏枯草中迷迭香酸的含量以比较夏枯草质量的优劣，为夏枯草的质量控制方法提供一定的依据。方法：采用高效液相法测定迷迭香酸色谱条件为：采用Betasil C18（250mm×4．6mm，5μm）；流动相：甲醇-1％甲酸（40：60）流速：1．0ml／min；柱温：25℃；检测波长：330nm；结果：迷迭香酸在0．44-8．80μg范围内线性关系良好；r=0．99999；平均回收率为98．17％，RSD％为1．02％。结论：该方法简单、准确并且专属性强。     

清眩软胶囊的质量控制研究

目的建立清眩软胶囊的定性鉴别和定量测定方法。方法采用TLC法对本方中的川芎、荆芥穗和薄荷进行定性鉴别;采用HPLC法对方中主要成分欧前胡素进行测定。Agilent C18色谱柱（250mm×4．6mm,5um）;以乙腈-水（42．5：57．5）为流动相;检测波长300nm,柱温为35℃,体积流量为1．5mL／min。结果薄层色谱斑点清晰,阴性样品无干扰。欧前胡素在1．09-54．55μg／mL与峰面积呈良好的线性关系,平均回收率为97．0％,RSD值为0．77％（n=6）。结论所建立的定性和定量方法结果可靠、准确,重现性好,无干扰,可用于清眩软胶囊的质量控制。     

感冒清热颗粒挥发油特征图谱及薄荷酮和胡薄荷酮的含量测定

目的:建立感冒清热颗粒中挥发油的特征图谱并测定其中薄荷酮和胡薄荷酮的含量。方法:Agilent HP-5毛细管柱(30 m×320 mm,0.25μm);进样口、检测器温度250℃;柱温:初始温度40℃,保持1 min,7℃.min-1升至100℃,保持12min,3℃.min-1升至150℃,保持8 min,10℃.min-1升至240℃,保持2 min;载气为氮气;流速:1 mL.min-1;不分流。结果:特征图谱中标定了6个共有峰。薄荷酮进样量在0.0211～0.422μg,胡薄荷酮进样量在0.0371～0.743μg内均呈良好的线性关系(r=0.9999);平均回收率分别为101.0%和97.5%,RSD均小于3%。结论:建立的气相色谱特征图谱及含量测定方法可从定性和定量两方面控制感冒清热颗粒中的挥发性成分,方法准确、可行,可作为感冒清热颗粒的质量控制方法。     

荆芥挥发油及其主要成分抗流感病毒作用研究

目的:观察荆芥挥发油及其主要成分薄荷酮、胡薄荷酮在鸡胚内及小鼠体内的抗流感病毒作用。方法:分别以预防(鸡胚感染病毒前1h给药)和治疗(鸡胚染毒后1h给药)两种给药方式,经鸡胚尿囊腔和卵黄囊两种给药途径将不同浓度的药物注入鸡胚,孵育48h后收取鸡胚尿囊液进行血凝试验来评价药物在鸡胚内的抗病毒效应。分别以预防(小鼠感染病毒前5d开始给药,连续5d)和治疗(小鼠感染病毒后当天开始给药,连续5d)方式给药,计算肺指数来评价药物的体内抗病毒效应。结果:胡薄荷酮0.1×2-8ml/胚剂量经尿囊腔预防给药后鸡胚尿囊液的血凝滴度明显降低;荆芥挥发油0.05×2-15ml/胚剂量经尿囊腔治疗给药后尿囊液血凝滴度明显降低;荆芥挥发油0.1×2-12、0.05×2-12、0.025×2-12ml/胚剂量与胡薄荷酮0.1×2-8、0.05×2-8、0.025×2-8ml/胚剂量经卵黄囊预防给药后尿囊液血凝滴度明显降低;荆芥挥发油0.1×2-12、0.05×2-12ml/胚、薄荷酮0.05×2-6ml/胚剂量及胡薄荷酮0.1×2-8、0.05×2-8、0.025×2-8ml/胚剂量经卵黄囊治疗给药后尿囊液血凝滴度明显降低。荆芥挥发油0.226ml/kg、薄荷酮0.5ml/kg、胡薄荷酮0.19 ml/kg剂量治疗方式给药明显降低病毒感染小鼠的肺指数。结论:荆芥挥发油及其主要成分胡薄荷酮在鸡胚内具有较好的抗流感病毒作用,且以卵黄囊途径给药效果为优,薄荷酮的抗病毒效应不明显。荆芥挥发油、薄荷酮及胡薄荷酮治疗方式给药对流感病毒感染小鼠有良好保护作用,但预防给药效果不明显。     

A Study on Inhibitory Effect of Spica Prunellae Extract on T Lymphoma Cell EL-4 Tumour

The objective of the study was to investigate the in-vivo anti-tumour activity of Spica prunellae extract, and to preliminarily explore the possible mechanism of in-vivo anti-tumour effect of Spica prunellae extract. Tumour inhibition rate and tumour apoptosis-related protein status were determined using the mice model of transplanted T lymphoma cell EL-4 tumour, and by immunohistochemical method. The results revealed that Spica prunellae extract showed certain tumour inhibitory effect, and compared with the model group. Tumour weight in Spica prunellae high-dose group was highly significantly different (P<0.01). Tumour weight in Spica prunellae medium-dose group was also significantly different (P<0.05) compared with the model group. Spica prunellae high-dose group enabled the high expression of Bcl-2 protein (47.54%) and low expression of Bax protein (13.14%). The study concluded that Spica prunellae extract has certain in vivo anti-tumour effect, which may be achieved through regulation of apoptosis related proteins.     

荆芥挥发油的提取及包合工艺研究

目的:采用水蒸气蒸馏法提取荆芥挥发油,优选提取工艺及包合工艺。方法:以挥发油的提取量为考察指标,以加水量、浸泡时间、提取时间作为考察的3 个主要因素,通过 L9(34) 正交试验法优化荆芥挥发油最佳提取工艺。以挥发油与β-环糊精的比例、溶剂用量、时间为考察因素,优选挥发油的包合工艺。结果:最佳提取工艺为饮片加水12倍量,浸泡3小时,提取8小时; 最优包合工艺为挥发油与β-环糊精比例为1:10,加入溶剂量为3倍量,胶体磨碾磨时间为15min。结论:优选的提取工艺和包合工艺简便、快速、稳定、可行,可作为荆芥挥发油产业化生产工艺。     

夏枯草对乳腺疾病作用机制概述

夏枯草是唇形科植物夏枯草的干燥果穗,是一种具有多种功能的传统药食两用植物。夏枯草具清肝泻火、明目、散结消肿等功效,其味辛、苦,性寒,归肝、胆经,为常用的清热药,自古便在乳腺疾病的治疗中多有应用。随着社会生活的发展,乳腺疾病逐渐成为女性最主要的健康问题。中医所述乳痈、乳癖、乳岩等病皆属于现代乳腺疾病范畴,且认为肝经与之最为密切。现代药理研究表明夏枯草含有萜类、酚酸类、黄酮类等多种生物活性成分,具有抗炎、抑菌、调节激素、抗肿瘤等作用。夏枯草通过抑制p38丝裂原活化蛋白激酶(p38 MAPK)/核转录因子-κB(NF-κB)通路以起到抗乳腺炎的作用,干预雌激素受体的作用效果或调节血脂水平以治疗乳腺增生,通过促进乳腺癌细胞凋亡、抑制乳腺癌细胞的迁移、调控乳腺癌细胞的分裂等多种方式协同作用发挥治疗乳腺癌的效果。在查阅夏枯草相关文献时,发现夏枯草往往通过多途径、多靶点调控发挥药理作用,但大部分的研究并未明确其发挥药效的具体靶点,有待后续深入研究。该综述对夏枯草治疗各类乳腺疾病的作用及其机制进行总结,为夏枯草在临床上发挥更加广泛的治疗作用及其治疗乳腺疾病作用的深入研究提供参考。     

Does Thumb Immobilization Contribute to Scaphoid Fracture Stability?

Immobilization protocols for nondisplaced scaphoid fractures have included the elbow, wrist, and thumb. This study attempts to demonstrate whether or not immobilization of the thumb makes a difference in preventing motion at the scaphoid fracture site. Using six fresh frozen forearm specimens, a transverse waist scaphoid fracture was created through a dorsal approach. Metallic markers were imbedded on either side of the fracture. Sutures were secured to the flexor pollicus longus (FPL) and extensor pollicus longus (EPL). Each specimen was loaded in extension and flexion by attaching 50-g weights to the EPL and FPL, first with no casting, then with a short arm cast, and finally a short arm thumb spica cast. Angulation and displacement at the fracture site were measured in the coronal, sagittal, and axial planes utilizing image reconstructions from computed tomography. One-way ANOVA with repeated measures and Tukey–Kramer multiple comparison test post hoc analysis were used for statistical evaluation. There was no significant difference in fracture angulation or rotation between spica and short arm casts. There was a significant difference in angulation and rotation in all three planes when comparing between casting and no casting, p < 0.05. In our cadaveric model, wrist immobilization is crucial for nondisplaced scaphoid waist fractures, and short arm casting was just as effective as thumb spica casting in preventing fracture displacement.