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1.
R. Janknegt 《Pharmacy World & Science》1990,12(3):81-90
The microbiological, pharmacokinetic, toxicological and clinical aspects of aminoglycosides are reviewed. Aminoglycosides still have an important place in serious infections in neutropenic patients, endocarditis andPseudomonas aeruginosa infections, all in combination with beta-lactams. Monotherapy (with streptomycin) is indicated in less common diseases like tularaemia and bubonic plague. Several experimental studies support a oncedaily dosing regimen for aminoglycosides (comparable or better efficacy with less ototoxicity and nephrotoxicity). Only a very limited number of prospective comparative studies have been performed, and much more data on efficacy, development of resistance and toxicity is needed before once-daily administration can be recommended. The choice of an aminoglycoside should be based primarily on the local sensitivity patterns and cost. Differences in ototoxicity and nephrotoxicity are usually minor. If the acquisition costs of amikacin decline, it is to be expected that amikacin will be the aminoglycoside of choice. 相似文献
2.
目的 :探讨妊高征患者分娩期应用硫酸镁对母儿预后的影响。方法 :回顾性分析近 10年来在该院分娩的妊高征患者分娩期应用硫酸镁情况的临床资料。结果 :分娩的妊高征患者共 82 6例 ,其中分娩期硫酸镁治疗组 4 2 2例 ,未治疗组 4 0 4例。轻、中、重度妊高征分娩期硫酸镁用量分别为 :4 .33± 1.14 g ;5 .5 0± 1.6 4 g ;9.0 0± 1.70g。不同程度妊高征硫酸镁治疗组与未治疗组比较 ,产程、产后出血量及新生儿窒息率差异均无显著意义 (P >0 .0 5 ) ,而两组中轻、中度妊高征分别与重度妊高征比较 ,产后出血量差异均有显著意义 (分别P <0 .0 1,P <0 .0 5 )。硫酸镁配伍酚妥拉明预防子痫 (n =89)较单用酚妥拉明治疗组 (n =5 4 )子痫发生率显著降低 (P <0 .0 5 )。结论 :妊高征患者分娩期适量应用硫酸镁对母儿是安全的 ,有利于预防子痫等并发症。 相似文献
3.
茅力 《中国药科大学学报》1998,29(5):38-41
建立了用离子色谱法测定硫酸软骨素的方法。在6mol/L盐酸介质中将硫酸软骨素水解生成等摩尔的硫酸根离子,然后用AnionHC离子色谱柱进行分离,以硼酸钠/葡萄糖酸钠淋洗液为流动相,流速1.5ml/min,电导检测器检测。硫酸根离子在0.001~0.080mg/ml浓度范围内线性关系良好,相关系数为0.9994,最低检测浓度为0.0006mg/ml。样品中硫酸根离子的加样回收率为98.51%~99.52%,硫酸软骨素原料药和注射液及鲨鱼软骨粉胶囊中硫酸软骨素测定的相对标准偏差分别为0.25%,1.06%和1.96%。该方法已应用于多种硫酸软骨素制剂中硫酸软骨素的含量测定 相似文献
4.
高效液相色谱法测定硫酸奈替米星注射液的含量 总被引:4,自引:0,他引:4
目的采用高效液相色谱法测定硫酸奈替米星及注射液的含量。方法以μ-Diamonsil—C18为分析柱,流动相为1.5%的磷酸二氢钾-乙睛,流速为1.0ml/min,检测渡长为205nm。含量测定用峰面积外标法。结果硫酸奈替米星在0.1—1.6mg/ml浓度范围内呈现良好线性关系,r=0.9998.平均回收率为99.5%,RSD=0.4%(n=9)。结论本方法操作简便、快速,结果准确可靠,重现性好,均能很好地满足质量控制的要求,为开辟氨基糖苷类抗生素含量测定的仪器提供了一定借鉴。 相似文献
5.
Renal adaptation to changes in inorganic sulfate intake and age was studied by comparing sulfate uptake by proximal tubule
brush border membrane vesicles (BBMV) from guinea pigs of different ages on relatively high- or low-sulfate diets. Adult (>60
days) or young guinea pigs (<25 days) were fed either a control diet (0.28% sulfur content), a sulfur-free diet, or a high-sulfate
diet. After 5 days on the diet, BBMV were obtained and kinetic analysis of 35sulfate uptake was determined. In adult guinea pigs, the low-sulfate diet produced a significant increase in apparent maximal
velocity (V
max). In young guinea pigs, a lower sulfate intake did not appreciably increase V
max, but a high-sulfate intake produced a reduction in V
max. The affinity for sulfate (K
m) was not changed in either age group. The dietary sulfate intake did not alter sodium gradient dependent-D-glucose or 32phosphate V
max. In conclusion, our data indicate that renal inorganic sulfate BBMV uptake is regulated and responds to conditions of increased
need (i.e., during the growth phase in young animals and during periods of decreased sulfate availability in adult animals)
by increasing BBMV V
max.
Received September 9, 1994; received in revised form March 18, 1997; accepted March 20, 1997 相似文献
6.
P M van der Kraan B J de Vries E L Vitters W B van den Berg L B van de Putte 《Journal of orthopaedic research》1989,7(5):645-653
We have studied the effect of environmental sulfate concentration on the glycosaminoglycan synthesis of anatomically intact patellar cartilage of the mouse in vitro. Incubation of mouse patellae in medium with sulfate concentrations below 0.5 mM resulted in a diminished incorporation of sulfate but in unaltered incorporation of glucosamine. This suggested the synthesis of undersulfated glycosaminoglycans under these conditions. We characterized glycosaminoglycans synthesized at three different sulfate concentrations: a sulfate concentration physiological for the mouse (1.0 mM), a sulfate concentration in the range where sulfate incorporation was strongly diminished (0.1 mM), and an extremely low sulfate concentration (10 nM). Analysis of glycosaminoglycan disaccharides and DEAE anion chromatography of the glycosaminoglycans could not confirm the synthesis of undersulfated glycosaminoglycans at 0.1 mM. The chromatogram of glycosaminoglycans synthesized in medium containing 10 nM showed the presence of a very low sulfated glycosaminoglycan pool not observed at higher medium sulfate concentrations. Intermediately sulfated glycosaminoglycans were also synthesized during incubation with 10 nM sulfate. So, our data indicate that only very low sulfate concentrations in the medium lead to the synthesis of undersulfated glycosaminoglycans and that the sulfation mechanism of murine patellar cartilage chondrocytes does not seem to fit completely in an "all-or-nothing" pattern. 相似文献
7.
Objective and design: Myeloperoxidase (MPO) and proinflammatory cytokines play an important role in the development of inflammation. These markers
are generally measured using tedious ELISA procedures. In this study, a novel technique utilizing antibody conjugated quantum
dot nanoparticles was developed to detect Myeloperoxidase, Interleukin-1α (IL-1α) and Tumor Necrosis Factor-α (TNF-α) in vivo in the dextran sodium sulfate (DSS) model of experimental colitis.
Materials and methods: Colitis was induced in animals (n = 8 animals/group) by feeding 4% DSS solution ad libitum for seven to eight days. Quantum Dots (QDs) exhibiting fluorescence at various wavelengths were conjugated to MPO, IL-1α
and TNF-α polyclonal antibodies and tested in vivo at various stages of colitis. Tissue sections obtained were imaged with confocal microscope. The image intensity obtained
from the tissue specimen was correlated with clinical activity measured as Disease Activity Index (DAI).
Results: Myeloperoxidase, IL-1α and TNF-α were visualized with quantum dots on various days of disease. The intensity of quantum dots
increased with the increase in inflammation. The increase in intensity showed an excellent correlation with the DAI based
on the clinical parameters.
Conclusion: The study demonstrated that multiple biomarkers can be detected simultaneously and their quantitative expression correlated
well with clinical disease severity. This novel technology should facilitate design of a novel optical platform for imaging
various biomarkers of inflammation, early detection of acute and chronic disease markers and inflammation-mediated cancer
markers. This detection may also facilitate determination of therapeutic success.
Received 14 March 2007; returned for revision 8 May 2007; accepted by M. Parnham 27 June 2007 相似文献
8.
Fatemeh Jahanian Iraj Goli Khatir Hamed Amini Ahidashti Sepideh Amirifard 《Ethiopian journal of health sciences》2021,31(2):267
BackgroundAcute exacerbations of chronic obstructive pulmonary disease (AECOPD) are serious complications that often require immediate intervention in an emergency department (ED). The aim of this study was to investigate the effect of intravenous magnesium sulphate as an adjuvant in the treatment of AECOPD in the ED.MethodsIn a double-blind, randomized clinical trial, a total of 60 patients with AECOPD presenting to the ED of Imam Khomeini Hospital in Sari, Iran, were included. The study was conducted between September 2016 and February 2018. Eligible patients were randomly allocated into two groups of intervention and control. Patients in the intervention and control groups received intravenous infusion of magnesium sulfate (2 gr) or normal saline over 30 minutes, respectively. For all patients, Borgdyspnea score, forced expiratory volume in one second (FEV1) result and clinical variables of interest were evaluated before the beginning of the intervention, and also 45 minutes and 6 hours after the commencement of intervention.ResultsRegardless of time of evaluation, pulse rate (PR), respiratory rate (RR) and Borg score in intervention group was lower than control group. Also, FEV1 and SPO2 were greater in intervention group compared to control group. However, these differences were not statistically significant (between-subject differences or group effect) (p<0.001). The trends of FEV1, SPO2, PR, RR and Borg score were similar between two groups of study (no interaction effect; P>0.05).ConclusionAccording to the results of this study, it seems that using intravenous magnesium sulfate has no significant effect on SPO2, FEV1, RR, and PR of patients with AECOPD who presented to ED. 相似文献
9.
目的:探讨按中国药典(2000年版)抗生素微生物检定法测定硫酸卡那霉素含量的最后稀释液浓度范围;方法:应用ZY-300IV型抗生素抑菌圈面积测量分析仪,测定硫酸卡那霉素抑菌圈面积与对数剂量的线性关系范围;结果:测定硫酸卡那霉素含量时,最后稀释液浓度范围在5.5-20.4IU/ml内对数剂量与抑菌圈面积呈直线关系。 相似文献
10.
高效液相色谱法测定复方吲哚美辛栓中硫酸沙丁胺醇的含量 总被引:2,自引:0,他引:2
目的 :建立 HPL C法测定复方吲哚美辛栓中硫酸沙丁胺醇的含量。方法 :采用 Dinamonsil C1 8柱 ,5 μm,4 .6 mm×2 0 0 mm;流动相 :磷酸二氢钠溶液 (用磷酸调 p H3.10± 0 .0 5 ) -甲醇 (85∶ 15 ) ,流速 1.0 ml·min- 1 ,检测波长 2 2 4 nm。结果 :硫酸沙丁胺醇在 9.6~ 38.4μg· ml- 1 范围内呈良好的线性关系 ,r=0 .99997,回收率为 99.95 %。结论 :该法快速、灵敏、准确。 相似文献