利用噬菌体肽库技术获得与人Fas结合的多肽基序

目的　利用噬菌体随机肽库技术获得与人Fas胞外区结合的多肽及其多肽基序 ,观察多肽基序的生物学功能。方法　以人Fas胞外区与IgGFc段的融合蛋白Fas .Fc为筛选配基 ,筛选噬菌体随机九肽库 ;微量淘洗与ELISA相结合鉴定阳性克隆 ,DNA测序和分析。化学合成多肽进行竞争性ELISA以及细胞增殖抑制实验。结果　经过 4轮亲和筛选 ,微量淘洗鉴定 ,获得 4 2个阳性克隆 ;固定ELISA实验显示筛选到的噬菌体短肽能与Fas .Fc特异性结合 ,并呈剂量依赖关系 ;随机选取 13个阳性克隆进行DNA测序 ,其序列及出现几率分别为 :PRKARVDTS(2 / 13)、YKKKSLQVQ (2 / 13)、YKKKSMLQA(2 / 13)、SRKKYDQYA(4/ 13)、YARKIKPTA(2 / 13)和ARKKTEGAG(1/ 13)。经多重序列分析 ,获得多肽基序 : R/KKK A。在ELISA和竞争性ELISA实验中 ,化学合成多肽EGEFYKKKSM LQADPAK (P3)可抑制Fas与抗人Fas单抗Apo 1的结合 ,且呈剂量效应关系 ;P3不能抑制Fas与FasL的结合。细胞增殖实验表明 ,多肽可抑制Jurkat细胞增殖 ,且随多肽剂量的增加而加强。多肽与单抗Apo 1联合作用对Jurkat细胞增殖的影响与单独使用P3没有明显差别。结论　通过噬菌体随机肽库技术获得与Fas结合的多肽及其多肽基序 ,它们可能模拟了抗Fas抗体Apo 1对Fas的结合位点 ,为基于Fas凋     

Chemical,radiochemical, and radionuclide purity of eluates from different commercial fission99Mo/99mTc generators

Seven⁹⁹Mo/^99mTc generators (using fission⁹⁹Mo) obtained from seven different manufactures were studied in 1984 and 1985 to test the quality of the eluates. We present the findings concerning the elution efficiency, radionuclide purity,⁹⁹Mo breakthrough, radiochemical purity, pH, and aluminium content of the eluates. One generator was overloaded with⁹⁹Mo by about 40%, while one generator had^99mTc yields of only about 80%. The eluates generally (although with some exceptions) exhibited a high and satisfactory radionuclidic purity and good radiochemical purity. The low-level determination of⁹⁹Mo break-through using a commercially available dose calibrator with a⁹⁹Mo assay shiled indicated a misleadingly high⁹⁹Mo content. All of the eluates had pH values of between 5.0 and 5.5, and the aluminium content was always below the detection limit of 1 g per milliliter of eluate. The generators performed well and proved their capability of functioning as reliable sources of sodium pertechnetate Tc99m. In all cases, the pertechnetate produced met the requirements of the European Pharmacopeia.This report is based on work conducted within the scope of a research poject devised by the Ministry of the Interior of the Federal Republic of Germany (Hammermaier et al. 1985). The present report reflects the opinions of the authors and does not necessarily express the views of the Federal Ministry of the Interior     

Coronary artery bypass surgery for left main coronary artery disease.

The course of 146 consecutive patients with significant occlusive disease of the left main coronary artery who underwent coronary artery bypass surgery during a 4 year period is reviewed. Preoperatively, 11 patients were in New York Heart Association functional class II, 57 in class III and 78 in class IV. Seventy patients had progressive angina and 12 unstable angina. There were two operative deaths (surgical mortality rate 1.4 percent). Seven patients (4.8 percent) had a perioperative acute myocardial infarction. Complete follow-up has been achieved in the surgical survivors over an average period of 18.1 months; 77 percent of the surviving patients are completely asymptomatic and 19 percent are in functional class II. Four patients (2.8 percent) had a nonfatal late postoperative myocardial infarction and five (3.5 percent) died during the late postoperative period (3.3 percent annual mortality rate during a 2 to 47 month follow-up period). Postoperative cardiac catheterization studies performed in 35 patients an average of 12.1 months postoperatively revealed 78 percent of 80 grafts and patency of at least 1 graft in 93 percent of patients. Results of 42 (89 percent) of 47 near maximal treadmill stress tests were abnormal preoperatively compared with results of 14 (26 percent) of 54 postoperatively; in 74 percent of patients having both a preoperative and postoperative stress test, abnormal preoperative test results converted to normal after surgery. This study suggests that direct myocardial revascularization may offer an effective means of improving both the quality and duration of life in a patient with significant occlusive disease of the left main coronary artery.     

Giant cell (cranial) arteritis: a clinical review

Giant cell arteritis is a disease of the elderly which is more common than previously recognized. It is important to be aware of this condition because treatment effectively relieves symptoms and prevents serious complications. The disease is suggested when an elderly patient complains of constitutional symptoms, headache, jaw claudication, or the musculoskeletal manifestations of polymyalgia rheumatica. Abnormalities in temporal arteries or other cranial arteries, or evidence of large vessel involvement may be detected by physical examination. A markedly elevated sedimentation rate in association with other clinical features of the disease strongly suggests giant cell arteritis, but a biopsy should be performed to confirm the diagnosis. Corticosteroid therapy should be started promptly in high doses in order to prevent blindness. Prolonged treatment with lower dose corticosteroids is generally necessary for up to 1 to 2 years, and sometimes longer, for continued symptomatic relief. Long-term follow-up of treated patients has demonstrated no detectable effect on survivorship.     

钼、锌对克山病心肌坏死的保护作用——钼、锌对缺氧性心肌损伤血浆cGMP血清Mg~(++)Ca~(++)含量变化的实验研究

本实验在分别饲以基础饲料,基础饲料加钼、加锌、加钼锌的基础上.用NaNO_2造成大鼠缺氧性心肌损伤,并与空白对照组进行对照。采用放射免疫法和原子吸收分光光度计测定各组大鼠血浆cGMP.血清Mg~(++)、Ca~(++)含量。结果表明基础饲料中加钼、加锌、加钼锌组较饲用基础饲料组大鼠血浆cGMP含量为低,血清Mg~(++)含量为高差异显著,其中基础饲料加钼锌组差异更为显著。分析其发生机理.说明了钼、锌对克山病心肌坏死具有保护作用,钼锌联合作用效果更为显著。     

Effects of dibutyryl adenosine 3',5'-monophosphate on hepatic metabolism of free fatty acids.

The effects of dibutyryl cyclic adenosine 3',5'-monophosphate (Bu2cAMP) on metabolism of free fatty acids by perfused livers from normal fed male rats were investigated. In one group of experiments, Bu2cAMP was added to the medium and infused at a constant rate to maintain concentrations of 0, 0.4, 1.0, 4.0, or 10. 0 X 10(-5) M nucleotide in the perfusate plasma, assuming the nucleotide was not metabolized by the liver. Oleic acid was infused as the complex with albumin at the rate of 124.3 mumoles/hr. Uptake of free fatty acid by the liver was identical in all groups. Production of ketone bodies, however, increased, and output of triglyceride decreased with increasing concentration of Bu2cAMP. The nucleotide also stimulated output of glucose. Maximal effects were observed when the concentration of Bu2cAMP was approximately 2-3 X 10(-5) M. The output of very low density lipoproteins, as judged by flotation in the zonal ultracentrifuge, was also diminshed by the nucleotide. In other experiments, 1-14C-oleate was infused (120.8 mumoles/hr) along with 2 X 10(-5) M Bu2cAMP, and the disposition of 14C into CO2, ketone bodies, and esterified lipids was evaluated. Bu2cAMP depressed the proportion of 1-14C-oleate converted to triglyceride and increased the fraction converted to ketone bodies and CO2. Not only was ketogenesis stimulated, but a larger proportion of the ketone bodies was derived from exogenous fatty acid.     

Biomedical Ti–Mo Alloys with Surface Machined and Modified by Laser Beam: Biomechanical,Histological, and Histometric Analysis in Rabbits

Purpose: In vivo bone response was assessed by removal torque, hystological and histometrical analysis on a recently developed biomedical Ti‐15Mo alloy, after surface modification by laser beam irradiation, installed in the tibia of rabbits. Materials and Methods: A total of 32 wide cylindrical Ti‐15Mo dental implants were obtained (10 mm × 3.75 mm). The implants were divided into two groups: 1) control samples (Machined surface – MS) and 2) implants with their surface modified by Laser beam‐irradiation (Test samples – LS). Six implants of each surface were used for removal torque test and 10 of each surface for histological and histometrical analysis. The implants were placed in the tibial metaphyses of rabbits. Results: Average removal torque was 51.5 Ncm to MS and >90 Ncm to LS. Bone‐to‐implant‐contact percentage was significantly higher for LS implants both in the cortical and marrow regions. Conclusions: The present study demonstrated that laser treated Ti‐15Mo alloys are promising materials for biomedical application.     

ICP-MS法测定酒石酸长春瑞滨中有害元素及催化剂银残留

目的：建立电感耦合等离子体质谱法测定酒石酸长春瑞滨中有害元素及催化剂银残留的方法。方法：用微波消解处理样品,电感耦合等离子体质谱内标法测定银、钒、铬、钴、镍、铜、砷、钼、镉、锡、锑、钡、铅、汞的含量。结果：上述14种元素的方法检出限分别为0.003、0.020、0.141、0.069、0.077、0.362、0.031、0.057、0.022、0.078、0.017、0.724、0.095、0.005 ng·mL^-1,线性关系良好（r≥0.9988）,回收率为86%~110%,RSD小于15%;同时,给出了此方法各元素的不确定度评定程序。结论：本方法采用在线加内标,可有效校正仪器漂移,抑制基体干扰,提高准确率,多元素测定便捷、准确、灵敏、可靠,可用于酒石酸长春瑞滨中有害元素及催化剂银残留的测定。     

数字化乳腺双靶X线机诊断乳腺疾病1600例分析

目的:探讨采用美国GE公司产钼铑双靶X线机摄片系统对乳腺癌的诊断价值.方法:通过数字化后处理方法,对1 600例乳腺疾病患者钼钝双靶X线摄片结果进行分析.结果:具备更高质量的乳腺图像,提高了乳腺疾病的诊断正确率,而且能够发现原有的单钯X线机所不易检出的疾病,如微小乳腺癌及微小钙化等,大大地提高了乳腺癌的早期检出率,而且其对人体辐射剂量减少,更具人性化,适合临床应用.