金属硫样蛋白5在乳腺癌组织中的表达及其与预后的关系

目的探讨金属硫样蛋白5(MTL5)在乳腺癌组织中的表达与患者预后的关系。 方法本回顾性研究采用在中国人类遗传资源共享服务平台(编号：2005DKA2130)获得的2005年1月至2012年9月初次手术、术前无肿瘤远处转、未经过新辅助治疗的77例乳腺癌患者的癌组织和癌旁组织标本进行免疫组织化学实验。术后随访截止至2016年1月,随访时间为3.3～11.0年。用χ²检验、Fisher精确概率法及非参数检验分析MTL5在乳腺癌组织中表达与临床病理特征的关系;用Cox比例风险回归模型分析MTL5表达对患者复发和死亡风险的影响;用Kaplan-Meier法进行生存分析并绘制生存曲线;采用log-rank检验比较不同MTL5表达患者的OS率和无复发生存率。 结果在纳入的77例成对样本中,排除脱片样本5例、癌组织和癌旁组织均无MTL5表达的样本29例、MTL5均有表达但差别无法判断的样本2例后,剩余41例,其中MTL5高表达(癌组织中MTL5表达高于癌旁组织)者21例,低表达(癌组织中MTL5表达低于癌旁组织)者20例。MTL5表达与年龄、腋窝淋巴结转移相关(P＝0.021;Z＝－2.281,P＝0.023),而与ER、PR、肿瘤大小、病理类型、HER-2无关(χ²＝0.034,P＝0.853;χ²＝0.042,P＝0.837;χ²＝1.177,P＝0.278;P＝0.663;P＝1.000)。单因素分析显示：腋窝淋巴结转移及MTL5表达为复发和死亡风险因素(复发风险：N₃期与N₀期比较,HR＝14.524,90%CI: 2.322～90.858,P＝0.004;MTL5高表达者比低表达者,HR＝8.752, 90%CI: 1.093～70.054,P＝0.041;死亡风险：N₃期与N₀期比较,HR＝8.469,90%CI: 1.495～47.988,P＝0.016;MTL5高表达者比低表达者,HR＝8.255, 90%CI: 1.031～66.081,P＝0.047)。多因素分析显示：高表达MTL5的乳腺癌患者复发风险更高,且可以作为独立风险因素(HR＝11.320, 95%CI：1.022～125.436,P＝0.048);同样,高表达MTL5的乳腺癌患者有更高的死亡风险,且可以作为独立风险因素(HR＝9.191, 95%CI：1.037～81.430,P＝0.046)。MTL5高表达者OS率及无复发生存率均低于MTL5低表达者(χ²＝5.652、6.084,P＝0.017、0.014)。 结论MTL5在乳腺癌组织中高表达可提示患者预后差,有潜力作为乳腺癌的治疗靶点。     

锌诱导扁藻类金属硫蛋白的初步研究

用不同浓度的ＺｎＳ４对绿藻门（ｃｈｌｏｒｏｐｈｔａ）、绿藻纲（ｃｈｌｏｒｏｐｈｙｃｅａｅ）、塔形藻亚目（Ｐｙｒａｍｉｍｏｎａ－ｄｌｎｅａｅ）、局藻科（Ｐｌａｔｙｍｏｎａｄａｃｅａｅ）、扁藻属（Ｐｌａｔｙｍｏｎａｓ）、亚心形扁藻（Ｐｌａｔｙｍｏｎａｓ ｓｕｂｃｏｒｄｉｆｃｒｍｉｓ）进行诱导培养,当 ＺｎＳＯ４浓度在１ｍｍｏｌ·Ｌ～－１以上时,随着ＺｎＳＯ４浓度的升高,扁藻的生长率逐渐降低,扁藻不能耐受８ｍｍｏｌ·Ｌ－１以上的ＺｎＳＯ４。对类ＭＴ提取液进行原子吸收测定,结果表明扁藻类ＭＴ的合成随着ＺｎＳＯ４浓度的升高而增大,诱导效果明显。用１ｍｍｏｌ·Ｌ～－１ＺｎＳＯ４诱导培养扁藻,每克湿藻能获得 ２．１ｍｇ类ＭＴ。     

Nature of copper and zinc compounds in tissues from a patient with Menkes kinky hair syndrome

Copper and zinc concentrations, and the nature of the copper- and zinc-binding proteins, were studied using tissues from a Menkes patient who had been given intravenous infusions of cupric acetate. The liver and brain copper contents were lower than in an untreated, non-Menkes control, and the spleen, intestine and kidney showed higher copper concentrations than control tissues. Zinc concentrations in all the organs (except the kidneys) from the Menkes patient were slightly lower than those the control child. Using Sephadex G-75 column chromatography of cytosols (105,000 x g supernatant), three copper- and zinc-containing peaks were eluted. In all the Menkes tissues studied, copper was prominent in peak 3. On the other hand, peak 3 was the smallest and peak 1 was the largest in the control tissues. Zinc predominated in peak 1 in both Menkes and control tissues, except for Menkes kidney. In this tissue peak 3 was again the largest. The copper- and zinc-binding material in peak 3 fractions from Menkes kidney was chromatographed on DEAE-cellulose columns. Three copper- and zinc-containing peaks were observed at the same positions as metallothionein from human adult kidneys.