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Camphor and m-cresol mixtures are used in antiseptic and anti-itching creams. No compendial method exists for these preparations. This paper reports a capillary gas chromatographic method using FID detection with 2,6-di-tert-butyl-4-methylphenol as internal standard on a 30 m×0.32 mm Supelcowax®-10 column (0.25 μm film) with helium as carrier gas. Ramped temperature programming was applied. The method allows simultaneous quantitation of camphor and m-cresol in the presence of o- and p-cresols, calamine and zinc oxide. Overall percent recoveries (±SD, n=9) of camphor, o-, p- and m-cresol from spiked placebo creams, at a labeled amount of 10 (w/w)% were 96.9±0.6, 98.2±0.6, 99.2±0.5 and 101.0±0.9%, respectively, and at a labeled amount of 1% were 96.7±0.6, 97.8±0.9, 97.8±0.6, and 100.3±1.0%, respectively. The recovery studies were carried out at ±30% of the labeled amounts. Linear peak area or height ratios were obtained (r>0.999) for camphor, o-, p- and m-cresol covering a concentration range of 10–200% of the labeled amount. Linearity (r>0.999) was also obtained for m-cresol when the relative concentration of o- and p-cresol was varied from 5 to 100% of the m-cresol concentration. The resolution between the ‘critical pair’ of p- and m-cresol was ≥1.1. The limit of quantitation was 23 pg for m-cresol and 9.3 pg for camphor using an injection split of 1:50. The repeatability (%RSD) for all compounds were <2% for peak area and <1.4% for peak height ratios. System suitability and robustness of the method were established. The method was successively applied to the assay of available commercial products and allows assay of camphor and the three cresol isomers. 相似文献
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本文建立了氢醌酯的薄层扫描测定方法。以甲苯:乙醚(4:1)为展开剂,氢氧化钠的醇溶液浸渍显色,λ_R=620nm,λ_?=375nm,对氢醌酯的含量进行了双波长薄层扫描法测定,结果回收率99.5%,CV=1.2%,表明该法简单,可行。 相似文献
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Cutaneous allergy to mustard in a salad maker 总被引:1,自引:0,他引:1
A case of allergic contact dermatitis to mustard (Brassica nigra) in a salad maker is reported. The sources of skin contact included a commercial salad cream, a vinegrette, and various members of the mustard family, Cruciferae. The Cruciferae family includes many salad vegetables and condiments which contain allergenic isothiocyanates. The extent of skin contact among salad makers and other food handlers may be significant, and sensitization to isothiocyanates from this large family of vegetables may be an important cause of food contact allergy in these individuals. 相似文献
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A double blind clinical trial was conducted with Egocort 1% Cream and plain aqueous cream applied to each half of the body in ten patients with eczema. The patients were assessed for erythema, papules, vesicles, scaling and pruritus at the beginning of treatment, after 7-14 days and again after a further 7-14 days.
The results show that Egocort 1% Cream is more effective in treating eczema than plain aqueous cream. The preparation was found pleasant to use and no side-effects were noted. 相似文献
The results show that Egocort 1% Cream is more effective in treating eczema than plain aqueous cream. The preparation was found pleasant to use and no side-effects were noted. 相似文献
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吡哌酸锌对小鼠烫伤感染的体表保护作用 总被引:2,自引:2,他引:0
目的观察吡哌酸锌软膏对烫伤后常见的感染菌绿脓肝菌、金黄色葡萄球菌的保护作用,并与阳性对照药磺胺嘧啶银霜、磺胺嘧啶锌软膏进行对比。方法取18~22克小鼠,在麻醉下将其尾部造成烫伤,然后再分别将其尾部没入绿脓肝菌和金黄色葡萄球菌液内,使其感染,尾部套上塑料管,将不同浓度的吡哌酸锌,磺胺嘧啶锌软膏及磺胺嘧啶银霜注入管内,封闭,连续观察14d内小鼠死亡现象拼进行显著性检验;结果综合计算法计算各药ED50为:(1)金黄色葡萄球菌,吡哌酸锌软膏ED50=69.84±18.27mg·kg-1,磺胺嘧啶银霜ED50=73.49±19.98mg·kg。(2)绿脓杆菌,吡哌酸锌软ED50=58.16±15.22mg·kg-1,磺胺嘧啶银霜ED50=80.1±2.24mg·kg-1。磺胺嘧啶锌软膏浓度低于0.4%即有明显抗菌作用。结论吡哌酸锋软膏、磺胺嘧啶银霜、磺胺嘧啶软膏均有明显抗菌作用,前两者相比没有显著差异,但吡哌酸锌软膏的抗菌作用比磺胺胺嘧啶锌强。 相似文献