高效液相色谱法测定降压袋泡茶中大黄酚的含量

目的建立降压袋泡茶中大黄酚的含量测定方法。方法采用高效液相色谱法,色谱柱为LichrospherODS柱(4.6×250mm,5μm)分析柱,流动相为甲醇-水(88∶12),检测波长为254nm,流速为1.0mL·min-1,柱温为30℃。结果本法精密度高,稳定性好,大黄酚进样量在0.0412~0.206μg范围内具有良好的线性关系(r=0.9997),平均加样回收率为101.7%,RSD=2.37%(n=9)。结论本方法简便、准确,重现性好,可作为该制剂的含量测定方法。     

Nutrient and mineral composition of dried mamaki leaves (Pipturus albidus) and infusions

Mamaki, Pipturus albidus, is currently one of the few native Hawaiian plants suitable for herbal tea preparation. This study reports the nutritional composition and mineral content of dried mamaki leaves and infusions. Three varieties and two sub-varieties, namely “hybrid purple”, “green”, “purple sub-variety 1”, “purple sub-variety 2” and “panaewa”, of mamaki were harvested in winters and summers over a 3-year period. Statistical analyses showed macronutrient values in dried mamaki leaves harvested in summer were significantly different from those in winter. The elemental values of phosphorus, magnesium, sodium, boron, copper, iron, manganese and zinc were also significantly different between the two seasons. Dried mamaki leaves contained higher amounts of ash, protein, fat and total fibers when compared with various commercial teas. Copper and iron levels in infusions from the leaves harvested in summer were significantly higher from those in winter. Phosphorus, potassium, sodium, boron, iron, manganese, and zinc values in steeped infusions of whole, ground, and crushed leaves were significantly different from each other. The dried leaves and infusions both contained the highest amount of calcium when compared to those in other commercial teas. The high calcium content found in infusions make the mamaki leaves a valuable commodity.     

HPLC法测定参花消渴茶中葛根素的含量

目的:建立参花消渴茶中葛根素的含量控制标准。方法:采用高效液相色谱法对处方中的粉葛进行含量测定,色谱柱用Discovery C18(4.6 mm×250 mm),流动相为甲醇-0.1%磷酸溶液(22∶78),检测波长250 nm。结果:葛根素在0.039 8～2.488 0μg范围内呈良好的线性关系(r=0.999 9)。平均回收率为98.6%,RSD为1.1%(n=6)。结论:该方法简便、准确。     

低酚酸银杏叶茶的制备

目的：研究低酚酸银杏叶茶的制备工艺。方法：市售银杏叶经过净选、洗净、烘干、粉碎及超临界二氧化碳萃取等工序可制备出低酚酸的银杏茶。结果：制得的银杏茶既保持有效成分,又降低了银杏酚酸的含量。结论：本工艺简便易行,具有可行性。     

别样茶降血脂研究进展

别样茶（Non—CamelliaTeas）是一类具有悠久的应用历史和应用传统,不属于山茶科（Theaceae）山茶属（Camellia）,迄今在民间仍广泛作茶饮的植物。现代化学研究表明,别样茶富含黄酮类、茶多酚、氨基酸、生物碱等多种化合物;现代药理和临床研究证明别样茶具有降压、降血脂、降糖、抗氧化等作用,是预防慢性代谢性疾病的很好的研究对象。通过查阅大量文献对别样茶降血脂方面进行了整理,此文从别样茶降血脂的化学成分及降血脂作用的机理进行阐述,为别样茶降血脂方面的深入研究和开发提供一定的参考。     

茶黄素通过上调环状 RNA叉头框蛋白 3表达调控结肠癌细胞增殖及凋亡

目的探讨茶黄素( TF)对结肠癌细胞增殖、凋亡的影响及其对环状 RNA叉头框蛋白 3(circ-Foxo3)的调控作用。方该研究于 2018年 10月至 2019年 12月进行,体外培养人结肠癌细胞 HCT-8,分别使用不同剂量的 TF处理细胞,分别将空载法体( pcDNA)、过表达 circ-Foxo3(pcDNA-circ-Foxo3)转染至 HCT-8细胞,分别将抑制物( si-NC)、抑制 circ-Foxo3(si-circ-Foxo3)转染至 HCT-8细胞,随后加入 TF处理细胞;采用噻唑蓝( MTT)法检测细胞增殖;流式细胞术检测细胞凋亡率;采用实时荧光定量 PCR(qRT-PCR)法检测 circ-Foxo3的表达量。结果 TF可明显降低细胞存活率［(100.00±0.12)%比( 82.93±5.51)%、     

灵源万应茶质量标准研究

目的 建立灵源万应茶的质量标准。方法 采用薄层色谱法对制剂中肉桂、丁香、积雪草、木香进行鉴别,用热漫法测定其醇漫出物含量。结果 薄层色谱法可对制剂中肉桂、丁香、积雪草、木香进行专属定性鉴别,醇浸出物不少于4％。结论 所建立的方法简便、准确,可用于灵源万应茶的质量控制。     

HPLC-ELSD法测定银杏叶茶中总内酯的含量

目的：采用RP-HPLC法测定低酚酸银杏叶保健茶中总内酯的含量。方法：采用AgilentC18（4.6mm×250mm,5μm）色谱柱,以甲醇-四氢呋喃-水（25：10：65）为流动相,柱温为30℃,蒸发光散射检测器检测。结果：银杏叶保健茶中银杏内酯A、银杏内酯B、银叶内酯C和白果内酯的进样量的对数与峰面积的对数线性关系较好,线性范围分别为0.485～9.701μg、0.460～9.204μg、0.501～11.899μg、0.935～18.703μg;银杏内酯A、银杏内酯B、银叶内酯C和白果内酯加样回收率分别为100.45%（RSD为2.79%）、98.29%（RSD为2.90%）、98.22%（RSD为2.53%）、101.32%（RSD为1.81%）。结论：本方法简便、准确、分离效果好,适合于银杏叶茶总内酯的含量测定。     

“通便茶”袋泡剂与汤剂的比较研究

袋泡剂为一种新剂型,具有溶出快、体积小、服用方便等优点。实验比较了全生药型“通便茶”袋泡剂与汤剂的质量,选择蒽醌类化合物的定性分析、大黄素的含量测定、袋泡剂的浸出速率测定、水溶性浸出物测定及对大鼠在体大肠内容物推进作用的影响等为指标。结果表明:“通便茶”袋泡剂与汤剂所含蒽醌类化合物的种类基本一致,游离大黄素袋泡剂为汤剂的2倍,结合型大黄素为3倍以上,水溶性浸出物袋泡剂为汤剂的1.9倍,对大鼠在体大肠内容物的推进作用两者无明显差异(p>0.05)。提示该处方可以用袋泡剂代替汤剂。     

Simultaneous determination of amino acids in different teas using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

Tea is a widely consumed beverage and has many important physiological properties and potential health benefits. In this study, a novel method based on supercritical fluid chromatography coupled with mass spectrometry (SFC-MS) was developed to simultaneously determine 11 amino acids in different types of tea (green teas, Oolong tea, black tea and Pu-erh tea). The separation conditions for the analysis of the selected amino acids including the column type, temperature and backpressure as well as the type of additive, were carefully optimized. The best separation of the 11 amino acids was obtained by adding water (5%, v/v) and trifluoroacetic acid (0.4%, v/v) to the organic modifier (methanol). Finally, the developed SFC-MS method was fully validated and successfully applied to the determination of these amino acids in six different tea samples. Good linearity (r ≥ 0.993), precision (RSDs ≤ 2.99%), accuracy (91.95%–107.09%) as well as good sample stability were observed. The limits of detection ranged from 1.42 to 14.69 ng/mL, while the limits of quantification were between 4.53 and 47.0 ng/mL. The results indicate that the contents of the 11 amino acids in the six different tea samples are greatly influenced by the degree of fermentation. The proposed SFC-MS method shows a great potential for further investigation of tea varieties.