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目的:研究中药肉苁蓉中异麦角甾苷标准品的制备及含量测定方法。方法:采用微晶纤维素色谱与半制备高效液相色谱组合分离技术,分离纯化异麦角甾苷,采用薄层色谱法和高效液相色谱面积归一化法检测纯度,波谱法鉴定其结构,反相高效液相色谱法测定其含量。结果:制备的化合物鉴定为异麦角甾苷,纯度为98.6%;含量测定在0.218~1.308μg,呈良好线性关系,r=0.9995,平均回收率为102.8%,RSD为2.60%,n=6。结论:该制备方法效率高,所得化合物纯度高,可用于大量制备;含量测肉苁蓉,定方法准确,简便,可靠,重复性好。 相似文献
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Kyoung Hee Kim Sungun Kim Min Young Jung In Hye Ham Wan Kyunn Whang 《Archives of pharmacal research》2009,32(1):7-13
The chromatographic separation of MeOH extract from Clerodendron trichotomum Thunberg leaves led to the isolation of three phenylpropanoid compounds. Using spectroscopic methods, the structures of these
compounds were determined as β-(3′, 4′-dihydroxyphenyl)ethyl-O-α-L-rhamnopyranosyl (1→3)-β-D-(4-O-caffeoyl)-glucopyranoside, acteoside (verbascoside) (1), β-(3′, 4′-dihydroxyphenyl)ethyl-O-α-L-rhamnopyranosyl (1→3)-β-D-(6-O-caffeoyl)-glucopyranoside, isoacteoside (2), β-(3′, 4′-dihydroxyphenyl) ethyl-O-α-L-rhamnopyranosyl (1→3)-β-D-glucopyranoside, and decaffeoylacteoside (3). We measured the anti-inflammatory activity of these three phenylpropanoid compounds both in vitro (DPPH Reduction Assay, TBARS Assay on Cu 2+-induced oxidized LDL, PGE2 assay) and in vivo (acetic acidinduced vascular permeability in mice and carrageenan-induced hind paw edema in rats). 80% methanol fraction
and acteoside had the activity. 相似文献
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