Determination of heavy metals in albumen of hen eggs from the Markazi Province (Iran) using ICP-OES technique

Increase of distribution of environmental contaminants such as heavy metals have been caused the knowledge of the safety and hygiene of food is very important, especially eggs, because of its role in the daily diet. There are very few studies about the investigation of the heavy metal contents in egg-white. In this study, six heavy metals include Aluminium (Al), Arsenic (As), Lead (Pb), Cadmium (Cd), Mercury (Hg), Antimony (Sb) in egg-white from 32 industrial poultry farms were investigated, by ICP-OES. All the samples were collected in all area of Markazi Province, Iran in autumn 2013. The mean concentrations of heavy metals in egg-white as follows: 0.119 for Al, 0.785 for As, 0.750 for Pb, 0.249 for Cd, 0.270 for Hg and 0.186?mg/kg for Sb. Also, the concentration of the some heavy metals were higher than maximum allowable concentration that probably it is associated to use pesticides and activities of industrial factories around the poultry farms.     

微波消解-ICP-OES法检测湘产朱砂中重金属的含量

目的建立微波消解-电感耦合等离子体原子发射光谱法（ICP-OES）检测湘产朱砂中Cd、Cr、Cu、Pb的含量,为矿物药的重金属检测提供参考。方法通过对比湿法消解,优选出微波消解作为朱砂样品的前处理方法,结合电感耦合等离子体发射光谱法（ICP-OES）的分析手段,对湖南产的11批朱砂样品中重金属含量进行检测。结果方法的线性相关系数为0.999 8～1.000 0,检出限为0.18～5.54μg·L^-1,RSD为0.55%～3.20%,回收率为84.30%～108.36%,经方法学验证适合同时测定朱砂中Cd、Cr、Cu、Pb的含量。结论该方法简便、准确,可同时检测朱砂中重金属Cd、Cr、Cu、Pb的含量。     

ICP-OES法测定深圳市面制食品中铝

目的建立面制食品中铝的ICP-OES测定方法,应用测定深圳市2009年10类面制品中铝含量,对其卫生质量状况进行评价。方法样品经硝酸高压消解后,用ICP-OES法测定其中的铝,卫生学评价按面制食品中铝限量卫生标准GB2762-2005进行。结果方法线性范围为0～10mg/L,线性相关系数为0.99996,方法检出限为2.2mg/kg,精密度为2.2%～3.8%之间,回收率在93.7%～103.8%之间。共检测10类491份面制食品,合格338份,合格率68.8%。其中饼、面包、汉堡包三类面制品中铝含量全部合格,油条等其它7类面制品合格率分布在22.2%～63.3%之间。结论 ICP-OES法测定面制品中铝准确可靠,线性范围广。深圳市部分面制品中铝含量超标严重,与制作过程中过量使用含铝添加剂有关。     

四种中药材中铅、鎘、砷、铜的含量测定

目的对四种中药材中的铅、镉、砷、铜的含量进行测定,为不同中药材的筛选提供一定的研究依据。方法采用电感耦合等离子体原子发射光谱仪(ICP-OES)进行样品检测。结果四种中药材中的铅、镉、砷、铜的含量均符合药典规定。结论该含量测定的方法方便、快捷、精密度高,可为药材的质量控制提供依据。     

ICP-OES法分析18种中药材中11种元素及其分布特征

目的:采用电感耦合等离子原子发射光谱法研究18种中药材中11种无机元素的含量,寻找不同中药材中元素分布特性和相关性,为控制中药材中的有关元素含量、保障中药材质量和安全提供依据。方法:收集18种不同产地的中药材,经微波消解后,电感耦合等离子体原子发射光谱法(ICP-OES)同时测定铅、镉、钴、铬、镍、铜、锰、铁、锌、钡和铝元素含量,并应用SPSS 15.0软件对测定结果进行统计分析。结果:建立的ICP-OES法线性相关系数大于0.9999,相对标准偏差小于10%,加标回收率在80%～120%之间。药材中铝、铬、钴、镉、铜、铁、锰、镍、锌元素间存在相关性;铝、铬、铁、锰、镍、锌元素在部分药材品种中具有一定规律性和特异性;铝、钡、铁元素在某些药材中存在含量异常现象。结论:ICP-OES法简便快速、准确、可靠,是中药材中部分元素测定的一种较好方法,为从安全性和有效性方面控制药材质量提供了一种解决方案。     

微波消解结合ICP-OES法测定大蓟不同炮制品中的微量元素

目的考察大蓟不同炮制品中微量元素含量的变化。方法采用微波消解样品,结合电感耦合等离子体发射光谱(ICP-OES)法对大蓟10种炮制品中Na、Mg、K、Ca、Mn、Fe、Cu、Zn 8种微量元素含量进行了测定。结果大蓟不同炮制品中,Ca、K、Fe、Mg的含量明显大于Na、Mn、Cu、Zn含量,其中,以Ca、K含量最为显著;大蓟经炒制后,微量元素含量均比大蓟生品含量高;Ca、K、Mn、Zn、Na在不同炮制品中的变化范围较大,分别是生品Ca、K、Mn、Zn、Na含量的0.99~2倍、1.01~2.37倍、1.07~2.29倍、1.07~1.87倍、1.07~3.66倍。结论大蓟炒炭后微量元素有较大变化与其炒炭后止血药效增强有一定的关系。     

The role of the N-terminus of mammalian copper transporter 1 in the cellular accumulation of cisplatin

The mammalian copper transporter 1 (CTR1) is responsible for the uptake of copper (Cu) from the extracellular space, and has been shown to play a major role in the initial accumulation of platinum-based drugs. In this study we re-expressed wild type and structural variants of hCTR1 in mouse embryo fibroblasts in which both alleles of mCTR1 had been knocked out (CTR1^−/−) to examine the role of the N-terminal extracellular domain of hCTR1 in the accumulation of cisplatin (cDDP). Deletion of either the first 45 amino acids or just the ⁴⁰MXXM⁴⁵ motif in the N-terminal domain did not alter subcellular distribution or the amount of protein in the plasma membrane but it eliminated the ability of hCTR1 to mediate the uptake of Cu. In contrast it only partially reduced cDDP transport capacity. Neither of these structural changes prevented cDDP from triggering the rapid degradation of hCTR1. However, they did alter the potency of the cDDP that achieved cell entry, possibly reflecting the fact that hCTR1 may mediate the transport of cDDP both through the pore it forms in the plasma membrane and via endocytosis. We conclude that cDDP interacts with hCTR1 both at ⁴⁰MXXM⁴⁵ and at sites outside the N-terminal domain that produce the conformational changes that trigger degradation.     

微波消解ICP—OES法测定当归中的微量元素

采用微波消解ICO-OES法对当归中的18种微量元素进行测定。结果表明,当归样品中元素含量高低依次是K、P、Ca、Mg、Fe、Al、Na、Zn、Cu、Mn、Sr、B、Ba、Cr、Ni、Pb、Cd、As,其中Pb、Cd、As含量甚微。除Pb、Cd、As外,各元素相对标准偏差(RSD)小于4.076%,各元素的加标回收率在96%~106%之间。     

Estimating the daily intake of essential and nonessential elements from lamb m. longissimus thoracis et lumborum consumed by the population in Missouri (United States)

This paper estimated the daily intake of essential and nonessential elements from lamb muscle longissimus thoracis et lumborum (m. LTL) available to the population in Missouri. Missouri grain-finished (n = 36), Missouri grass-finished (n = 40) and imported New Zealand grass-finished (n = 40) lamb m. LTL were purchased and assayed for their element composition. Total mercury in samples was quantified by atomic absorption spectrometry and other elements were analyzed by inductively coupled plasma-optical emission spectrometry. Analysis of certified reference materials (DORM-2 and TORT-2) gave recovery rates ranging from 86 to 108% and 85 to 104%, respectively. The abundance of essential elements in Missouri grain-finished and Missouri grass-finished lamb m. LTL followed the order: Potassium > phosphorus > sodium > magnesium > calcium > zinc > iron. The order in the imported New Zealand grass-finished group was: Potassium > phosphorus > sodium > calcium > magnesium > zinc > iron. Since many variables lacked normality, non-parametric (Kruskal–Wallis) comparison test was performed and the results suggested no significant difference (P > 0.05) in element composition among the lamb m. LTL groups. The estimated daily intake of 14 elements from consumption of 100 g lamb m. LTL posed no hazard to consumers.     

藏药珊瑚七十味丸中重金属含量及Hg元素化学形态研究＊

目的 测定珊瑚七十味丸（Ratanasampil，RNSP）中5种重金属元素的含量，并确定Hg元素存在的各种化学形态，为名贵藏药重金属含量及元素形态的确定提供准确的新型研究方法。方法 采用强酸消解法处理珊瑚七十味丸，利用电感耦合等离子体原子发射光谱（Inductively coupled plasma atomic emission spectrometry，ICP-OES）测定样品中不同重金属元素的含量，同时采用X射线光电子能谱技术（X-ray photoelectron spectroscopy，XPS）对样品中的Hg元素进行价态分析。结果 不同批次的珊瑚七十味丸中Hg、As、Cr、Cd及Cu这5种重金属元素含量存在一定的差异；Hg元素存在游离Hg2+、HgS、HgO共3种形式，且差异较大。结论 ICP-OES和XPS对药物中的5种重金属含量和Hg元素的价态进行的检测和分析，得到的数据较为准确、可靠，能够为珊瑚七十味丸的临床使用提供数据上的参考依据，也为传统道地藏药材中的重金属含量检测及价态分析提供了一种新的研究思路和方法。