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1.
Yohei Tomaru Hiroshi Kamada Yuta Tsukagoshi Shogo Nakagawa Kenta Tanaka Ryoko Takeuchi Yuki Mataki Shumpei Miyakawa Masashi Yamazaki 《Journal of orthopaedic science》2019,24(1):159-165
Background
On April 1, 2016, the Ministerial ordinance was enforced, and musculoskeletal examination of the extremities was made mandatory. From 2008, the University of us started musculoskeletal direct examination. To expand the examination, from 2016, we started to use the marksheet-type questionnaire. This study aimed to report the results of a musculoskeletal examination and investigate the association between musculoskeletal examination and age/gender and reports the reliability of the collected questionnaire data.Methods
Direct musculoskeletal examination was performed in K school by 7 orthopedic surgeons. A marksheet-type screening questionnaire was distributed to all the elementary and junior high school students in Tsukuba and Hitachiomiya cities. The rates of abnormal findings for scoliosis, standing flexion, full squatting with the heels on the floor, general joint laxity, and standing on one leg, torticollis, and flat feet were calculated. We compared the results of the questionnaire and direct examination and calculated sensitivity, specificity, and odds ratio.Results
A total of 1844 students in K school had direct examination, and 22,494 questionnaires were able to correct in Tsukuba and Hitachiomiya cities. The rates of abnormal findings in direct examination/questionnaire in scoliosis, standing flexion, full squat, general joint laxity, standing on one leg, torticollis and flat foot were 18.7% (344/1842)/5.1% (1094/21441), 20.2% (372/1841)/26.6% (5817/22078), 6.2% (114/1832)/6.9% (1516/22101), 7.5% (1648/22252), 4.9% (1100/22077), 2.2% (31/1844)/1.2% (272/21687), and 12.5% (231/1842)/8.7% (1785/20871), respectively. Sensitivities of the questionnaire for scoliosis, stand flexion, full squatting, torticollis, and flat feet were 16.8% (53/316), 67.9% (250/368), 48.2% (55/114), 18.9% (7/37), and 32.2% (65/202), respectively.Conclusion
We reported the result of musculoskeletal examination. Accuracy and reliability of this questionnaire were not satisfactory. To perform high quality musculoskeletal examinations, we will aim to increase the quality of screening methods. 相似文献2.
3.
Leoncio Garrido Bettina Pfleiderer Bruce G. Jenkins Carol A. Hulka Daniel B. Kopans 《Magnetic resonance in medicine》1994,31(3):328-330
Studies using magnetic resonance spectroscopy in human volunteers to evaluate their livers in vivo and to analyze their blood in vitro suggest that there are measurable amounts of silicon compounds in the blood of some women with implants and that there is migration of silicone to other organs such as the liver. 相似文献
4.
P. A. da Silva M. M. S. Boffo I. G. de Mattos A. B. S. Silva J. C. Palomino A. Martin H. E. Takiff 《Clinical microbiology and infection》2006,12(3):293-296
Rapid, accurate and inexpensive methods are essential to detect drug-resistant Mycobacterium tuberculosis and allow timely application of effective treatment and precautions to prevent transmission. The proportion method, the MTT and Alamar Blue redox methods, and the D29 mycobacteriophage assay, were compared for their ability to detect resistance to isoniazid and rifampicin. When tested against a panel of known M. tuberculosis strains, the redox methods and the D29 assay showed good sensitivity and specificity compared to the proportion method, suggesting that they could be useful alternatives for identifying multidrug resistance in M. tuberculosis. 相似文献
5.
6.
目的研究肺腺癌细胞生长环境及转移性与黏附分子CD44v6和CD29的表达关系。方法将起源相同、转移性不同的两个肺腺癌细胞系AGZY和Anip分别用简便肿瘤多细胞球体(MTS)培养法培养,并设常规单层贴壁细胞培养对照。通过倒置显微镜、扫描及透射电镜观察MTS形成情况,并用免疫组化法分别对MTS及贴壁细胞上CD44v6和CD29表达进行检测。结果MTS培养成功,贴壁细胞与MTS在细胞结构及细胞连接结构上相似,两种MTS在形态及结构上差异无显著性。免疫组化结果显示,CD29在高转移性的Anip细胞及其MTS上呈阳性表达;在低转移性的AGZY细胞及其MTS上阴性表达。CD44v6在Anip和AGZY细胞及MTS上均呈阳性表达,差异无显著性。贴壁细胞与MTS上两种黏附分子表达均无差异。结论成功建立了一种简易制备MTS的方法。细胞生长方式(单层贴壁与MTS)可能不影响CD44v6和CD29的表达。CD29表达可能与肺腺痛转移性相关;CD44v6表达可能与肺腺癌转移无关。 相似文献
7.
Yamamoto N Yang M Jiang P Xu M Tsuchiya H Tomita K Moossa AR Hoffman RM 《Clinical & experimental metastasis》2003,20(7):633-638
We have established stable, bright green fluorescent protein (GFP)- or red fluorescent protein (RFP)-expressing HT-1080 human
fibrosarcoma clones. These cell lines showed similar cell proliferation rates and high-frequency experimental lung metastasis.
The HT-1080-GFP and -RFP clones enable simultaneous real-time dual-color imaging in the live animal. HT-1080 cells were transduced
with retroviral vectors containing GFP or RFP and the neomycin resistance gene. Stable transformants were selected stepwise
with G418 up to 800 μl/ml. Subsequently, high GFP- or RFP-expressing clones, HT-1080-GFP or HT-1080-RFP, respectively, were
selected. 3×106 cells from each clone were mixed and injected into the tail vein of SCID mice. The cells seeded the lung at high frequency
with subsequent formation of pure green and pure red colonies as well as mixed yellow colonies with different patterns visualized
directly on excised lungs. The lung metastases were also visualized by external fluorescence imaging in live animals through
skin-flap windows over the chest wall. Lung metastases were observed on the lung surface of all mice. SCID mice well tolerated
multiple surgical procedures for direct-view imaging via skin-flap windows. Real-time metastatic growth of the two different
colored clones in the same lung was externally imaged with resolution and quantification of green, red, or yellow colonies
in live animals. The color coding enabled determination of whether the colonies grew clonally or were seeded as a mixture
with one cell type eventually dominating, or whether the colonies grew as a mixture. The simultaneous real-time dual-color
imaging of metastatic colonies described in this report gives rise to the possibility of color-coded imaging of clones of
cancer cells carrying various forms of gene of interest.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
8.
R. Nitschke J. Leipziger R. Greger 《Pflügers Archiv : European journal of physiology》1993,423(3-4):274-279
Cell swelling induced by hypotonic solution led to an osmolality-dependent increase in intracellular Ca2+ activity ([Ca2+]i) in HT29 cells. At moderate reductions in osmolality from 290 to 240 or 225 mosmol/l in most cases only a small monophasic increase of [Ca2+]i to a stable plateau of 10–20 nmol/l above resting [Ca2+]i was observed. Lower osmolalities resulted in a triphasic increase of [Ca2+]i to a peak value. In a first phase after the volume change, lasting 20–40 s, [Ca2+]i increased slowly by about 30 nmol/l. Thereafter [Ca2+]i increased more rapidly within 20–30 s to a peak value. This peak was 189±45 nmol/l (190 mosmol/l, n=9) and 243±41 nmol/l (160 mosmol/l, n=20) above resting [Ca2+]i. The peak was then followed by a decline of [Ca2+]i over the next 2–3 min to a stable plateau value of 28±6 (n=6) and 32±11 nmol/l (n=11) above resting [Ca2+]i at 190 and 160 mosmol/l, respectively. The plateau lasted as long as the hypotonic solution was present. Under Ca2+-free bath conditions the peak value for the cell-swelling-induced [Ca2+]i transient was reached significantly later (60–100 s, compared to 40–60 s under control conditions). The peak values under Ca2+-free conditions were not significantly lower. This indicates that the [Ca2+]i peak was mostly of intracellular origin. No [Ca2+]i plateau phase was observed under Ca2+-free bath conditions. With the use of the fura-2-Mn 2+ quenching technique an increased Ca2+ influx induced by hypotonic cell swelling was shown (160 mosmol/l; n=4). This influx started immediately after or simultaneously with the cell swelling and preceded the [Ca2+]i peak for more than 50 s.This study was supported by DFG grant Gr 480/10. 相似文献
9.
Barbara Biedziak Justyna Dąbrowska Anna Szponar-Żurowska Ewelina Bukowska-Olech Aleksander Jamsheer Ewa Mojs Jennifer Mulle Rafał Płoski Adrianna Mostowska 《American journal of medical genetics. Part A》2023,191(1):205-219
Many unbalanced large copy number variants reviewed in the paper are associated with syndromic orofacial clefts, including a 1.6 Mb deletion on chromosome 3q29. The current report presents a new family with this recurrent deletion identified via whole-exome sequencing and confirmed by array comparative genomic hybridization. The proband exhibited a more severe clinical phenotype than his affected mother, comprising right-sided cleft lip/alveolus and cleft palate, advanced dental caries, heart defect, hypospadias, psychomotor, and speech delay, and an intellectual disability. Data analysis from the 3q29 registry revealed that the 3q29 deletion increases the risk of clefting by nearly 30-fold. No additional rare and pathogenic nucleotide variants were identified that could explain the clefting phenotype and observed intrafamilial phenotypic heterogeneity. These data suggest that the 3q29 deletion may be the primary risk factor for clefting, with additional genomic variants located outside the coding sequences, methylation changes, or environmental exposure serving as modifiers of this risk. Additional studies, including whole-genome sequencing or methylation analyses, should be performed to identify genetic factors underlying the phenotypic variation associated with the recurrent 3q29 deletion. 相似文献
10.
Since its conception, several techniques have been described for laparoscopic appendicectomy. We describe a technique which utilizes a 5-mm bipolar forceps designed to coagulate and cut tissues at the same time; 50 pediatric patients underwent laparoscopic appendicectomy for acute appendicitis using this stripping and teasing technique. No bleeding complications occurred. There were only two postoperative abscesses in the series. We believe that the bipolar laparoscopic striptease appendicectomy technique as developed by the senior author is safe, quick, and effective, even for severe appendicitis. 相似文献