荷移分光光度法测定诺氟沙星含量

本文研究了诺氟沙星与P-苯醌荷移反应条件,确定在乙醇介质中,在室温下反应50min可获得稳定的络合物,其λ_(max)=490nm;组成比为1:1表观摩尔吸光系数ε=4.13×10~3。应用拟定的方法测定制剂中诺氟沙星含量,获得满意的结果。     

高效液相色谱法测定峰丁麻滴眼剂中盐酸麻黄碱含量

目的　建立高效液相色谱法测定蜂丁麻滴眼剂中盐酸麻黄碱含量的方法。　方法　采用YWGC18分析色谱柱 ( 4 6mmID× 2 5 0mm ,粒径 10 μm) ,C18保护柱 ( 4 6mmID× 5mm ,粒径 10 μm) ,流动相 10 %乙腈 ( 0 5 %三乙胺 ,磷酸调pH3 0 ) ,流速 1 0ml/min ,检测波长 2 0 8nm ,测定对照品和样品色谱图 ,记录盐酸麻黄碱色谱峰面积 ,计算其含量。　结果　盐酸麻黄碱的理论板数为 10 0 0 0。回归方程 :y =90 5 878 16 15 1738x ,r=0 9999,线性范围 0 4 0 32～ 1 6 13μg。平均回收率为 99 2 % (RSD1 6 % )。　结论　该法操作简便 ,结果准确 ,可用于测定蜂丁麻滴眼剂中盐酸麻黄碱的含量     

血清亚硝酸盐的快速测定

目的：建立一种无需去蛋白的血清亚硝酸盐的快速测定方法。方法：加入蛋白活化剂后，直接用Ｇｒｉｅｓ试剂显色分光光度测定。结果：方法的检测下限为０．２μｍｏｌ／Ｌ，相对标准差为５．１３％～８．８６％，回收率为９４．５％～９９．８％（平均为９７．８％）。血清ＮＯ－２浓度为１４．３８±０．２５μｍｏｌ／Ｌ时，批内变异系数为１．７５％，ＮＯ－２浓度为５．６９±０．２５μｍｏｌ／Ｌ时，批间变异系数为４．４６％。测定５５例正常人血清，得到血清中ＮＯ－２浓度的平均值分别为５．３６±２．１６ｕｍｏｌ／Ｌ；对糖尿病病人血清测定结果表明，亚硝酸盐浓度明显高于正常人。与去蛋白的Ｇｒｉｅｓ试剂分光光度法比较，结果无统计学差异。结论：用蛋白活化剂替代蛋白沉淀剂，减少操作步骤和干扰，测定准确、简便、快速，适用于血清中ＮＯ－２的测定。     

血清硝酸盐的快速测定

目的建立一种血清硝酸盐的快速测定方法.方法血清经去蛋白后,直接在酸性条件下用麝香草酚显色分光光度法测定.结果方法的检测下限为0.05μg,相对标准差为4.6%～9.6%,回收率为90.0%～95.9%(平均为93.6%).血清硝酸盐氮含量为0.13±0.011μg时,批间变异系数为8.6%.结论本法快速、简便、灵敏、特效性好,适用于血清中NO3-测定.     

Spectrophotometric determination of pyrrole-like substances in urine of rat and man: An assay for the evaluation of 2,5-hexanedione formed fromn-hexane

Male Wistar rats exposed to atmosphericn-hexane excreted in their urine substances which gave rise to absorption spectra like those of pyrroles after the reaction with Ehrlich's reagent. A simple spectrophometric assay was developed to determine these pyrrole-like substances in urine. Their excretion kinetics were evaluated by exposing rats for 8 h to atmosphericn-hexane concentrations between 50 and 3000 ppm. The dose-response curve revealed saturation kinetics according to Michaelis-Menten, V_max being 1.12 [E ⁵²⁶ml urine/8 hn-hexane exposure] and Km, the atmosphericn-hexane concentration at V_max/2, being 250 ppm. The excretion of pyrrolelike substances closely correlated with that of 2,5-hexanedione measured by Fedtke and Bolt (1987). Pyrrole-like substances were also found in the urine of a male volunteer. When exposing the person for 3 h to atmosphericn-hexane at a concentration of 146 ppm (equivalent to 55 ppm/8 h) the excreted amount was twice the background value. Due to the sensitivity of this assay it is possible to determine pyrrole-like substances in urine according to the present German MAK or US TLV conditions forn-hexane (50 ppm/8 h).     

Laryngeal cancer and blood selenium levels

Blood selenium concentrations in 43 patients with laryngeal cancer and 47 normal subjects were determined by atomic absorption spectrophotometry. No significant differences in selenium values were observed in sera from the cancer patients when compared with controls, but there were significant differences between patients with advanced (T4) supraglottic cancers and the normal controls. These results suggest that lower blood selenium levels in this latter group of laryngeal cancer patients may be a consequence of their disease rather than its cause.     

复方益康唑滴耳液的制备及质量控制

目的：制备复方益康唑滴耳液并测定其含量。方法：以甘油和乙醇作为溶煤进行配制。采用双波长分光光度法在波长263.8nm和247.9nm处测定硝酸益康唑含量，在波长295.1nm处测定甲磺酸左氧氟沙星含量，并观察了该滴耳液的稳定性和刺激性。结果：硝酸益康唑和甲磺酸左氧氟沙星的平均回收率分别为99.6%(RSD=0.5%)和101.6%(RSD=0.4%)，刺激性小，稳定性好，结论：本制剂制备工艺简便，质控方法准确可靠，性质稳定，适应临床应用。     

盐酸左氧氟沙星乳膏的的制备

目的：研制盐酸左氧氟沙星乳膏。方法：拟订处方组成及制备工艺，建立紫外分光光度法用于含量测定。结果与结论：乳膏的制备工艺可行，质量稳定，用UV法测定盐酸左氧氟沙星的含量，方法可行，重现性好，可用于医药制剂的质量控制。