脂肪乳对血液细胞CBC参数及Diff分类参数检测的影响

目的 观察脂肪乳对血液细胞分析结果的影响.方法 在体外将健康志愿者血液样本以及生理盐水与一定比例的脂肪乳混合,配制系列浓度的血液/脂肪乳混合液以及生理盐水/脂肪乳混合液,于血液细胞分析仪上进行测试,观察不同浓度脂肪乳对CBC参数、Diff分类参数的影响.结果 随着脂肪乳浓度的增高,血液/脂肪乳混合液的CBC参数测定值渐进升高,Diff分类参数中的淋巴细胞分类比例呈下降趋势而单核细胞比例逐渐增高；而生理盐水/脂肪乳混合液中白细胞、血红蛋白和PLT-O明显升高.结论 脂肪乳会对血液细胞分析仪多个参数的测定产生影响,可以通过测定结果的一些特征筛选可疑含脂肪乳标本.     

Long-Circulating Multiple-Emulsion System for Improved Delivery of an Anticancer Agent

Stealth multiple emulsions of small size, containing 6-mercaptopurine, were prepared by incorporation of sphingomyelins (SM) and monosialogangliosides (GM1) in the oily phase and by coating with lipid-grafted polyethylene glycol (PEG-PC). The three types of "stealth" multiple emulsions were characterized for size distribution, zeta potential, viscosity, encapsulation efficiency, drug release, and stability. Drug disposition studies were performed with formulated multiple emulsions to assess "stealth" behavior. The tissue distribution studies were carried out with the PEG grafted multiple emulsion. The results suggest that PEG-PC-coated multiple emulsions are superior as prolonged release and extended bloodcirculating carriers compared to multiple emulsions bearing either SM or GM1.     

鱼油脂肪乳与中长链脂肪乳对胃肠道肿瘤患者肠外营养术后细胞免疫及炎症反应的影响

【目的】探讨鱼油脂肪乳与中长链脂肪乳肠外营养对胃肠道肿瘤患者术后细胞免疫及炎症反应的影响。【方法】选取本院2013年10月至2014年10月收治的胃肠道肿瘤术后行肠外营养患者70例,随机分为对照组和观察组,每组各35例。两组患者均接受等氮、等热量的全肠外营养支持：对照组患者术后24 h内、7 d补充中长链脂肪乳;观察组患者术后24 h内、7 d补充中长链脂肪乳联合鱼油脂肪乳。检测两组患者术后d1及d8静脉血中T淋巴细胞亚群（CD3、CD4、CD8细胞）百分比及CD4／CD8比值、单核细胞数目、单核细胞人类白细胞DR抗原（HLA‐DR）表达百分比、白细胞介素‐1β（IL‐1β）、白细胞介素‐6（IL‐6）以及C反应蛋白（C reactive protein ,CRP）含量,并统计两组患者术后并发症的发生情况。【结果】术后d1,两组患者细胞免疫功能指标及炎症反应指标相比较,差异无统计学意义（ P ＞00．5）;术后d8,观察组患者CD3、CD4细胞百分比、CD4／CD8比值、单核细胞数目和 HLA‐DR表达百分比显著高于对照组,差异具有统计学意义（ P ＜00．5）;观察组患者IL‐1β、IL‐6以及CRP含量均显著低于对照组,差异具有统计学意义（ P ＜00．5）;观察组并发症的发生率显著低于对照组,差异具有统计学意义（ P ＜00．5）。【结论】胃肠道肿瘤患者术后以鱼油脂肪乳营养支持能够提高患者细胞免疫功能,减轻患者术后的炎症反应,降低术后并发症的发生率,值得临床推广应用。     

鸦胆子油乳剂治疗恶性胸腔积液58例疗效观察

目的 :观察鸦胆子油乳剂胸腔内注射治疗恶性胸腔积液的疗效和副作用。方法 :对 5 8例恶性胸腔积液患者 ,采用胸腔穿刺或放置引流管排尽胸水后 ,胸腔内每次注入鸦胆子油乳剂 5 0～ 1 0 0 ml,隔 3日 1次 ,最多 4次为 1疗程 ,1月后观察疗效和副作用。结果 :鸦胆子油乳剂治疗恶性胸腔积液的有效率为 96.5 % ( 5 6/5 8) ,不良反应为发热 ( <38℃ )和轻度胸痛各 1例。结论 :鸦胆子油乳剂腔内注射治疗恶性胸腔积液疗效显著 ,副作用轻 ,值得进一步推广使用。     

抗癌药/碘油乳剂及阿霉素微球栓塞治疗中晚期肝癌的研究

用~(131)I-阿霉素/碘油乳剂对3例肝癌患者肝动脉栓塞后,肝区γ照相证实抗癌药选择性地存留在肿瘤组织,作用时间约3d。为获得更理想的栓塞和药物缓释作用,我们制备了阿霉素微球,体外释药显示微球释药明显慢于乳剂,第一阶段9h释药49.9%,第二阶段24h释药60%。对19例肝癌患者分别采用乳剂和微球-乳剂混合剂栓塞治疗,由于碘油的栓塞和载体作用,使肿瘤坏死、缩小、AFP下降、病人症状减轻、生存时间延长,与手术联合使用,对不能切除的肝癌患者提供了肿瘤切除的希望。     

聚苯乙烯核-聚(丙烯酰胺-丙烯酸)壳荧光素微球制备方法的研究

目的:为制备性能良好的核壳结构荧光微球,探讨了加料方式、引发剂选择、交联剂使用、pH调节、浓度配比和溶胀处理等反应条件对形成壳结构的效果以及形态学上的影响。方法:通过以乳液聚合得到的荧光素微球为核,以丙烯酰胺、丙烯酸为壳结构的聚合单体,制得了聚苯乙烯核-聚(丙烯酰胺-丙烯酸)壳荧光素微球。结果:荧光显微镜显示:粒径均一,分布范围7~8μm。红外吸收光谱证实了聚(丙烯酰胺-丙烯酸)壳结构以及表面酰胺基的存在。该种子聚合反应的最佳条件为:在不使用交联剂的前提下以偶氮二异丁腈为引发剂,经过40 h的乙醇溶胀处理,70℃反应3 h,反应过程pH值在6~7之间。该荧光微球的平均载药量和包封率分别为25.14%和90.21%,其荧光释放率40 h后稳定在30%左右。结论:用此法制备的微球具有良好的荧光性能和明显的核壳结构,分散性和稳定性良好,其载药量和包封率也相对稳定,并具有良好的缓释性能。     

Surface energetics analysis of artificial blood substitutes

The surface and interfacial tensions for fluorocarbon aqueous emulsions used in artificial blood substitutes are evaluated over a wide range of surfactant concentrations. The surface tension of the nonionic surfactant in water is directly correlated to the interfacial tension between aqueous and fluorocarbon phases by a surface energetics model of isolated dispersion and polar contributions to interfactial work of adhesion and interfacial tension. This study shows that the polymeric surfactant which is an A-B-A triblock copolymer, where A is polyethylene oxide and B is polypropylene oxide, displays prominent surface and interfacial tension transitions at c=1×10⁻⁷ and c=0·10 weight fraction detergent in water. The c=10⁻⁷ transition is shown to produce a prominent change in the extensibility of the fluorocarbon to water interface and formation of a strong interfacial film that enhances emulsion stabilisation.     

Formulation and evaluation of carrot seed oil-based cosmetic emulsions

The present study deals with the evaluation of antiaging potential of carrot seed oil-based cosmetic emulsions. Briefly, cosmetic emulsions composed of carrot seed oil in varying proportions (2, 4, and 6% w/v) were prepared using the hydrophile–lipophile balance (HLB) technique. Coconut oil, nonionic surfactants (Tween 80 and Span 80), and xanthan gum were used as the oil phase, emulgent, and emulsion stabilizer, respectively. The formed emulsions were evaluated for various physical, chemical, and biochemical parameters such as the zeta potential, globule size measurement, antioxidant activity, sun protection factor (SPF), skin irritation, and biochemical studies. The zeta potential values ranged from ?43.2 to ?48.3, indicating good stability. The polydispersity index (PDI) of various emulsion formulations ranged from 0.353 to 0.816. 1,1-Diphenyl-2-picrylhydrazyl- (DPPH) and nitric oxide-free radical scavenging activity showed the antioxidant potential of the prepared carrot seed oil emulsions. The highest SPF value (6.92) was shown by F3 having 6%w/v carrot seed oil. Histopathological data and biochemical analysis (ascorbic acid (ASC) and total protein content) suggest that these cosmetic emulsions have sufficient potential to be used as potential skin rejuvenating preparations.     

复方氯己定达克罗宁乳膏含量测定方法研究

目的建立反相高效液相层析(RP-HPLC)法同时测定复方氯己定达克罗宁乳膏中两种成分含量的方法,用于制定复方氯己定达克罗宁乳膏的质量标准。方法采用C18(250 mm×4.6 mm,5μm)色谱柱,流动相,乙腈∶0.05moL/L磷酸二氢钾溶液(含0.07%庚烷磺酸钠,0.4%三乙胺,用磷酸调pH至3.0),流速1.0 mL/min,检测波长284 nm,柱温25℃。结果盐酸氯己定、盐酸达克罗宁的线性范围分别为0.100 03～2.000 60μg和0.015 18～0.303 60μg,相关系数均为0.999 99,回收率(n=9)分别为99.83%和99.89%,相对标准偏差(RSD)分别为0.41%和0.14%。结论该方法简便、准确,可作为控制复方氯己定达克罗宁乳膏中盐酸达克罗宁、盐酸氯己定含量的质量标准。     

抗霉毒剂HD乳剂防治烧伤创面感染的研究

本研究通过动物体表创面同体对照,烧伤感染模型实验治疗及20例烧伤病人局部外用药对照的治疗观察,进一步证实3％抗霉毒剂RD乳剂为一种具有抗霉菌、抗细菌的有效药物。