Genetic polymorphisms of 20 STR loci in a Chaoshan group from Jieyang,China

We obtained allelic frequencies and forensic parameters of 20 short tandem repeat (STR) loci (D3S1358, FGA, D5S818, D7S820, CSF1PO, D16S539, D19S433, vWA, D8S1179, D18S51, D13S317, TPOX, TH01, D2S1338, D12S391, D1S1656, D21S11, D6S1043, Penta D, Penta E) from 529 unrelated individuals in Jieyang Han population using PowerPlex® 21 (Promega, Madison, Wi, USA). The relationship between the Jieyang Han group and other Han populations was studied and the results showed that the Jieyang Han population had the closest genetic relationship with the Fujian Han population.     

广东省潮汕地区单中心头颈部鳞状细胞癌患者人乳头瘤病毒初步检测分析

目的探讨广东省潮汕地区单中心头颈部鳞状细胞癌(HNSCC)患者人乳头瘤病毒(HPV)感染及亚型状况。方法收集2014年12月至2016年12月汕头大学医学院附属肿瘤医院167例HNSCC患者的肿瘤原发灶标本。采用免疫组织化学(IHC)法检测肿瘤组织中p16蛋白的表达,以肿瘤细胞p16蛋白阳性率≥76%为判断HNSCC存在HPV的依据,分析p16蛋白与患者临床病理因素的关系。采用原位杂交法(ISH)检测肿瘤组织中是否存在HPV 16、18 DNA;应用RNAscope法检测肿瘤组织中18种常见的高危HPV亚型(HPV HR 18)RNA的表达情况,分析p16蛋白阳性细胞比例≥50%的肿瘤组织中HPV HR 18阳性情况。结果患者p16蛋白强表达率为7.2%(12/167);低龄组(<50岁)p16蛋白强表达率高于高龄组(≥50岁)[17.2%(5/29)比5.1%(7/138),χ^2=5.321,P=0.021];口咽癌组p16蛋白强表达率高于非口咽癌组[29.4%(5/17)比4.7%(7/150),χ^2=14.019,P<0.01];性别、烟酒嗜好及肿瘤分期分层患者间p16蛋白强表达率差异均无统计学意义(均P>0.05)。ISH检测示,全部HNSCC原发灶均未发现HPV 16、18 DNA,重复实验结果一致。RNAscope法检测示,肿瘤细胞p16蛋白阳性率≥50%的19例患者中,3例(15.8%)肿瘤组织HPV HR 18 RNA阳性。结论潮汕地区HNSCC患者的HPV阳性率较低,以口咽癌患者最高,且呈年轻化趋势。潮汕地区HNSCC主要致HPV亚型可能并非HPV 16、18,而可能是包括HPV HR 18中的其他致病亚型。     

Polymorphisms of HLA-A and HLA-B genes in genetic susceptibility to esophageal carcinoma in Chaoshan Han Chinese

Esophageal carcinoma (EC) occurs at high rate in Chaoshan region of southern China. Human leukocyte antigen (HLA) polymorphism has been implicated in risk for various cancers. To investigate the impact of HLA-A and HLA-B polymorphisms on susceptibility to EC, a case–control study was conducted among 206 patients with esophageal squamous cell carcinoma and 524 controls from Chaoshan Han population. HLA-A and HLA-B polymorphisms were genotyped by polymerase chain reaction-sequence-specific primers. Genotypic association tests for dominant, recessive, and additive models, and haplotypic association were calculated using unconditional logistic regression. A*11 was identified in a recessive model as an only allele strongly associated with EC risk (odds ratios [OR]= 2.10, 95% confidence interval [CI]= 1.33–3.31) even after correction for multiple test. The haplotypes A*02-B*46 (OR = 1.53, 95% CI = 1.04–2.24) and A*11-B*51 (OR = 2.29, 95% CI = 1.20–4.40) showed association with increased risk for EC, whereas A*11-B*58 (OR = 0.00, 95% CI = 0.00–0.82) was associated with decreased risk, though the significance of these haplotypes was lost after correction. This is a first association study at genetic level identifying HLA-A and HLA-B-related variations in genetic susceptibility to EC among Chaoshan population. The variation pattern is likely to be EC-specific because it is different from that observed for nasopharyngeal carcinoma in the same study population and might, at least in part, explain the high rate of EC in this ethnic group.     

潮汕地区椎动脉型颈椎病中医证候分布的调查分析

目的：观察潮汕地区椎动脉型颈椎病患者的中医证候分布规律。方法：对在潮汕地区居住两年以上的300例该病患者开展调查、结合中医辨证进行分型并对调查结果进行统计分析,以了解本病在该地区人群的分布状态及痰浊上蒙型所占的比例。结果：痰浊上蒙型比例最高,（90例,30.0％）。结论：痰浊上蒙型颈椎病的发病率高于其他各型颈椎病,有必要进一步开展从“痰浊”论治椎动脉型颈椎病的前瞻性临床研究,为该病的治疗拓宽思路。     

Reproducibility of a Semi-quantitative Food Frequency Questionnaire in Chaoshan Area,China

Objective: To examine reproducibility of assessed intake of foods and nutrients according to a semi-quantitativefood frequency questionnaire (SQFFQ) in adult doctors and nurses residing in Chaoshan area of China. Subjects:The SQFFQ was administered first in October to December of 2004 to 120 adult doctors and nurses living in Chaoshanarea of China and was then re-administered to 102 three months later between January and March of 2005 (SQFFQ1 and SQFFQ 2). Methods: Reproducibility was evaluated in terms of consumption of 10 food groups and energyand 34 macro- and micro-nutrients based on the SQFFQ from the 102 doctors and nurses. Results: For intake offoods, Pearson’s correlation coefficients (CCs) with log-transformation and energy adjustment (minimum – median- maximum) range from 0.43 (eggs) – 0.84 - 0.90 (teas). Spearman’s rank CCs with energy adjustment rangedfrom 0.77 (cereals) – 0.84 - 0.94 (milks). Kappa statistics with energy adjustment ranged from 0.53 (vegetables) -0.63 - 0.82 (teas). For consumption of nutrients, Pearson’s correlation coefficients (CCs) with log-transformationand energy adjustment (minimum – median - maximum) range from 0.83 (docosahexaenoic acid and oryzanin) -0.88 - 0.90 (linolenic acid, vitamin A, folic acid, vitamin E, calcium, sodium, selenium and magnesium). Spearman’srank CCs with energy adjustment ranged from 0.81 (oryzanin and vitamin C) – 0.86 – 0.90 (sodium). Kappastatistics with energy adjustment ranged from 0.49 (protein) - 0.60 - 0.77 (sodium). Conclusion: Substantially highreproducibility was observed; it is possible to use the tailored, relatively simple, but comprehensive, self-administeredSQFFQ to facilitate assessment of the association between lifestyle and health/disease in large-scale epidemiologicalstudies.     

潮汕地区的木本抗癌高等植物自然概况及开发利用

报道了潮汕地区25科39种木本抗癌高等植物,其中裸子植物1科2种,被子植物24科37种。对主要的抗癌植物及其产地和药用情况亦做了说明,以期为资源的利用和开发提供参考,并提出了相应的建议和措施。     

高分辨熔解曲线检测潮汕汉族人群的载脂蛋白E基因多态性

目的研究载脂蛋白E（ApoE）基因单核苷酸多态性各等位基因及基因型在潮汕汉族人群中的分布频率,比较其在不同种族间分布的差异。方法采用高分辨熔解曲线分型技术检测100例潮汕地区汉族人群的ApoE基因rs429358和rs7412多态性。PCR．测序法作为金标准进行比对。结果在潮汕地区汉族人群中存在ApoE基因rs429358和rs7412多态性。潮汕人的ApoE基因型分布以E3／E3最常见,其次为E4／E3。与非洲及欧洲人群比较,ApoE基因多态性的分布频率均存在显著性差异（尸l〈0．05）;而与中国汉人比较差异无显著性（P〉0．05）。结论采用高分辨熔解曲线法进行apoE基因多态性检测,其操作简便省时,不易交叉污染;获得的各种基因类型特征性强,适用于临床快速诊断分型和遗传学人群研究。ApoE的3种常见等位基因频率分布存在地域差异。     

潮汕地区农村留守儿童孤独感与社会支持的关系研究

目的探讨潮汕地区农村留守儿童孤独感与社会支持的关系。方法运用儿童孤独量表、社会支持评定量表和自编的一般情况问卷,随机抽取潮州、汕头、揭阳3市农村地区的五年级和初一年级学生进行问卷调查。结果 1在社会支持总分方面,父母均在外打工组儿童得分显著低于父亲在外打工组和对照组儿童;在客观支持维度上,父母均在外打工组和对照组儿童得分均低于父亲在外打工组儿童;23组留守儿童与对照组儿童的孤独感存在显著差异;3初一年级留守儿童在社会支持总分、客观支持维度得分显著高于五年级留守儿童;4留守儿童的孤独感与社会支持总分、客观支持、主观支持和对支持的利用度都呈显著负相关。结论潮汕地区农村留守儿童孤独感与社会支持呈负相关。     

BCL-2凋亡基因多态性与潮汕食管鳞癌的关联研究

目的：初步探究B细胞淋巴瘤因子2基因-938(C/A)位点的多态性和潮汕食管鳞癌患者临床表型、易感性、淋巴结转移风险的关系。方法本研究收集了110例潮汕食管鳞癌患者(2012年1月至2014年12月)的癌组织标本以及相关临床资料和110例健康人群的正常食管黏膜标本。采用聚合酶链式反应-限制性片段长度多态性分析(PCR-RFLP)技术,分析两组中BCL-2基因-938(C/A)位点多态性;采用流式细胞术及细胞免疫荧光技术,分析食管鳞癌细胞周期和凋亡情况,对BCL-2蛋白的表达进行定量分析。采用χ2检验比较不同基因组BCL-2基因-938(C/A)多态性位点基因型的分布频数与食管鳞癌临床分期及淋巴结转移的关联,BCL-2蛋白表达与食管癌蛋白肿瘤病理特征的关系。结果将食管鳞癌的一些临床表型进行关联分析,对照组AA基因型频率显著高于试验组(P<0.05)。将试验组按不同临床特征分层后的基因型频率分布提示,TNM分期为I期+II期者,其AA基因型频率明显高于TNM分期为Ⅲ期+Ⅳ期者,差异具有统计学意义(P<0.05),表明含AA基因型的患者病情轻于不含此基因型的患者。结论 BCL-2的表达高低与食管癌患者的病情严重程度呈现正相关,且BCL-2的高表达与食管癌患者的不良预后有关。     

CYFRA21-1对潮汕地区食管鳞状细胞癌的诊断价值

目的 探讨细胞角蛋白19片段(CYFRA21-1)在潮汕地区食管鳞状细胞癌诊断中的应用价值.方法 采用电化学发光法分别检测健康对照组(30例)和食管鳞状细胞癌患者(90例)术前、术后第10天血清中CYFRA21-1和癌胚抗原(CEA)浓度,并对患者进行随访,复发者再检测血清中CYFRA21-1和CEA浓度.结果 食管癌组术前CYFRA21-1和CEA水平明显高于对照组[分别为(5.13±2.75)μg/L比(1.61±0.70)μg/L,(3.14±1.60)μg/L比(1.93±0.74)μg/L,均P<0.05].食管癌组术前CYFRA21-1和CEA水平高于术后第10天[分别为(5.13±2.75)μg/L比(2.44±0.65)μg/L,(3.14±1.60)μg/L比(2.55±0.92)μg/L],差异有统计学意义(均P<0.05).患者术前血清CYFRA21-1阳性率(58.9%,53/90)高于CEA(25.6%,23/90),差异有统计学意义(χ2=18.00,P<0.05);复发者血清CYFRA21-1阳性率(72.5%,29/40)高于CEA(32.5%,13/40),差异有统计学意义(χ2=12.50,P<0.05).结论 血清CYFRA21-1是食管鳞状细胞癌诊断和判断复发的辅助指标.

Abstract：

Objective To investigate the clinical significance of cytokeratin 19 fragments (CYFRA21-1)in patients with esophageal squamous cell carcinoma in Chaoshan area.Methods The electrochemiluminescence (ECLIA) was used to detect the serum concentrations of CYFRA21-1 and carcinoembryonic antigen(CEA)in the healthy controls and the patients with esophageal squamous cell carcinoma (ESCC) before surgery and 10 days after analyzed.Resuits In Chaoshan area.the serum levels of CYFRA21-1 and CEA in patients with ESCC were obviously higher before surgery than that 10 days after sugery(P<0.05).The positive rate of CYFRA21-1 before surgery(58.9%,53/90)was higher than that of CEA(25.6%,23/90),with statistical significance(χ2=18.00,P<0.05).The positive rate of CYFRA21-1(72.5%,29/40)in the recurrence of esophageal tumors was higher than that of CEA(32.5%,13/40),with statistical significance(χ2=12.50,P<0.05).Conclusion Serum CYFRA21-1 is the secondary indicator of the diagnosis and recurrence of ESCC.