146例女性不孕症的病因分析

目的 分析女性不孕的病因。方法 收集1990-2000年我院女性不孕症146例，不孕时间2年以上。结果 不孕症的主要原因是子宫因素，感染因素及内分泌失调，原发性不孕以子宫因素为主，继发性不孕则以感染因素为主。结论 女性不孕症的原因常是多因素的，必须采用综合治疗，才能提高妊娠率。     

Integrinα4在骨肉瘤中的表达与意义

Objective： To investigate the expression of integrin α4 in osteosaxcoma and significance. Methods- Forty-six patients with osteosarcoma （Enneking Ⅰ-Ⅲ） were analyzed for the expression of α4 integrin subunit using immunohistochemical method. Results： Twenty-nine （63.04%） of 46 samples demonstrated positive （＋-＋＋） integrin α4 expression. Loss expression of integrin α4 was observed in the patients with advanced Enneking stage （P=0.0040） and with metastatic disease at presentation （P=0.0158）. Integrin α4 expression correlated with cell differentiation, the level of malignancy and the invasive behavior of osteosaxcoma. Conclusion： The loss expression of integrin α4 subunit might be a predictor indicating the invasive potential of osteosarcoma and play a role in metastasis of osteosaxcoma patients.     

颅咽管瘤切除术后水钠代谢紊乱机制研究

目的探讨颅咽管瘤术后水钠代谢紊乱的原因和最佳处理方式。方法对102例经胼胝体切开穹窿间入路切除巨大颅咽管瘤的病人，记录术后尿量、血电解质、抗利尿激素（ADH）、醛固酮（ALD）、皮质醇水平，比较术后激素水平变化与水、钠代谢紊乱的关系。结果本组均出现水、钠代谢紊乱，术后2周完全恢复52例，4周基本恢复33例，6周恢复12例，需长时间人工调整电解质水平5例。术后ADH、ALD和皮质醇的不足是导致术后水钠代谢紊乱的主要原因。结论颅咽管瘤切除术后水、钠代谢紊乱与手术损伤下丘脑有关，紊乱类型与ADH、ALD和醛固酮的缺乏情况有关；及时给予相应激素及对症治疗，可获满意疗效。     

侯江红教授治疗小儿慢性湿疹亚健康状态的经验

侯江红教授擅长运用调脾和胃法调治处于亚健康状态的小儿，治疗小儿慢性湿疹，注重从“健脾和胃、消食清热”法入手，每获良效。     

大学生篮球运动员集训期间部分淋巴细胞亚群和Th1/Th2细胞因子mRNA表达变化分析

目的:探讨大负荷体能训练对大学生篮球运动员细胞免疫功能的影响。方法:14名男子大学生篮球运动员进行为期16周的集训,在训练期间分次对运动员外周血淋巴细胞亚群(CD3+、CD4+/CD8+淋巴细胞比值、NK细胞比例)和Th1/Th2细胞因子 mRNA表达进行检测。结果:在两次分别持续了6周的训练期后,运动员外周血CD3+淋巴细胞数于增加后出现显著下降,从第7周的峰值(59.74±7.18)下降至第16周的最低值(52.02±7.92)(P<0.001);CD8+细胞数第16周(31.15±6.25)与第3周(37.98±7.05)相比显著下降(P<0.05);NK细胞数从第7周的最高值(19.62±5.21)下降至第14周的最低值(14.41±7.93)(P<0.05);IL-4 mRNA表达训练后与基础值(6.56±0.71)相比显著增加,从第7周的7.04±0.35和第14周的7.30±0.25,直至第16周达到最大值7.36±0.45(P<0.05),其他指标的变化不具有统计学意义。结果提示,大负荷训练使运动员细胞免疫功能削弱,Th1/Th2平衡向Th2方向漂移。     

肺性脑病与多器官功能障碍临床分析

目的：分析43例肺性脑病与多器官功能障碍的关系，以便早期采取预见性防治措施。方法：把43例肺性脑病时伴发的功能障碍器官个数与死亡率的关系及酸碱、电解质测定进行统计列表分析，结果：肺性脑病100％并发器官功能障碍，最常受损器官：肺、心、脑、肝或肾，受损器官数目越多，病情越严重，肺性脑病时，呼酸代酸，低钠，低氯血症明显增多。结论：肺性脑病100％伴发多器官功能障碍，随功能障碍器官数目的增加，死亡率亦增加，酸碱失衡及电解质紊乱与多器官功能障碍有关。     

建立一种粪便细菌学检验的新方法--肠道菌谱分析(初步报告)

目的 建立一种以粪便标本细菌菌谱分析为主要内容的细菌学检验方法。方法 取粪便标本直接涂片做革兰染色，镜检观察标本中细菌数量、种类及比例，并进行显微照相；对粪便标本进行菌群培养，分离病原菌，并与镜检结果进行对照；将照片进行分类。建立菌群失调和病原菌感染的图谱。同时以正常大便菌谱作对照。结果 因菌群失调导致的腹泻。直接镜检表现为细菌数量和种类普遍减少，或某一类细菌增多或减少，或呈单一种类细菌；由某种病原菌引起的腹泻，直接涂片多数只能见到革兰阴性杆菌，且细菌数量少或极少，一般均与培养结果相符。结论 粪便细菌菌谱分析能直接或间接为临床提供腹泻患者的肠道微生态资料，尤其能准确地提示因各种原因造成菌群失调而导致的腹泻，为临床诊断和制定治疗方案提供快捷、科学的依据。     

双歧杆菌三联活菌肠溶胶囊联合阿奇霉素序贯疗法治疗对肺炎支原体肺炎腹泻患儿胃肠炎症的调节作用

目的观察双歧杆菌三联活菌肠溶胶囊联合阿奇霉素序贯疗法治疗对肺炎支原体肺炎腹泻患儿胃肠炎症的调节作用。 方法将肺炎支原体肺炎伴腹泻的患儿106例均分为对照组和观察组。对照组采用阿奇霉素序贯疗法治疗,观察组在对照组基础上给予双歧杆菌三联活菌肠溶胶囊治疗。比较两组疗效和肠道菌群失调发生率。比较两组胃肠激素、降钙素原(PCT)、C反应蛋白(CRP)、中性粒细胞百分比(NEUT%)、嗜酸性粒细胞计数(EOS)水平。 结果观察组总有效率高于对照组(P＜0.05)。治疗后,两组胃肠激素和PCT、CRP、NEUT%、EOS较治疗前下降(P＜0.05),且观察组低于对照组(P＜0.05)。观察组肠道菌群失调发生率低于对照组(P＜0.05)。 结论双歧杆菌三联活菌肠溶胶囊联合阿奇霉素序贯疗法治疗可减轻肺炎支原体并腹泻肺炎患儿炎症反应,调节胃肠激素,降低患儿肠道菌群失调的发生。     

Postnatal expression of transport proteins involved in acid–base transport in mouse kidney

The kidney plays a major role in maintaining and controlling systemic acid–base homeostasis by reabsorbing bicarbonate and secreting protons and acid-equivalents, respectively. During postnatal kidney development and adaptation to changing diets, plasma bicarbonate levels are increasing, the capacity for urinary acidification maturates, and the final morphology and distribution of intercalated cells is achieved. In adult kidney, at least two types of intercalated cells (IC) are found along the collecting duct characterised either by the expression of AE1 (type A IC) or pendrin (non-type A IC) where non-type A IC are found only in the convoluted distal tubule, connecting tubule and cortical collecting duct. Here we investigated in mouse kidney the relative mRNA abundance, protein expression levels and distribution of several proteins involved in renal acid–base transport, namely, the Na⁺/HCO₃ ^– cotransporter NBC1 (SLC4A4), the Na⁺/H⁺-exchanger NHE3 (SLC9A3), two subunits of the vacuolar H⁺-ATPase [ATP6V0A4 (a4), ATP6V1B1 (B1)], the Cl^–/HCO₃ ^– exchangers AE1 (SLC4A1) and pendrin (SLC26A4). Relative mRNA abundance of all transport proteins was lowest at day 3 after birth and increased thereafter in parallel with protein levels. The numbers of type A and non-type A IC in the cortical collecting duct (CCD) increased from day 3 to days 18 and 24, whereas the number of IC in the CCD with apical staining for the vacuolar H⁺-ATPase subunits a4 and B1 decreased from day 3 to days 18 and 24, respectively. In addition, cells with characteristics of non-type A IC (pendrin expression, basolateral expression of vacuolar H⁺-ATPase subunits) were found in the inner and outer medulla 3 days after birth but were absent from the medulla of 24-day-old mice. Taken together, these results demonstrate massive changes in mRNA and protein expression levels of several acid–base transporters during postnatal kidney maturation and also show changes in intercalated cell phenotype in the medulla during these processes.