Antitumor activity and antioxident role of Bauhinia racemosa against Ehrlich ascites carcinoma in Swiss albino mice

AIM: To study the enzyme activity of CYP2C18 variant with exon 5 skipped. METHODS: A full length CYP2C18 cDNA X1 and an exon 5 skipped variant CYP2C18 X2 were separately subcloned into mammalian expression vector pREP9 to transfect HepG2 cells. The expression of CYP2C18 mRNA in transgenic cells and human liver tissues were determined by RT-PCR. The enzyme activity of CYP2C18 to oxidate tolbutamide in postmitochondrial supernate (S9) fraction was determined by HPLC. The cytotoxicity of ifosfamide to transgenic cells was evaluated by MTT test. RESULTS: HepG2-CYP2C18 X1 cells showed strong expression of the full length CYP2C18 mRNA. On the other hand, HepG2-CYP2C18 X2 cells had only infinitesimal expression of the exon-skipped CYP2C18 as well as the full length CYP2C18, while non-transfected HepG2 cell only demonstrated an infinitesimal expression of the full length CYP2C18. The expression of CYP2C18 exons 2 to 7 was also analyzed by RT-PCR in 7 extratumoral liver tissues. Among them, 3 samples expressed on     

东山寻茶

朋友茶馆前植有几株紫荆,每年三月下旬,蓓蕾初放,别样红艳。一来是作赏景,二来亦是提醒,因为这几株紫荆花期总与春茶同步。今年三月刚入中旬,某次信步阳台,惊觉紫荆竟不知何时悄然绽开,正欲思及茶期,苏州东山制茶师张师傅打来电话告知,第一锅碧螺春已出炉。原来今年初春那大起大落过山车似的气温在意欲冲顶之时催早的     

热烈庆祝香港回归10周年——国际华夏医药学会与香港回归同行

1997年7月1日在香港合展中心前的广埸上，屹立着永远盛开的金色的紫荆花，象徵着香港是充满活力而又高度自治的国际大都市。     

“骨肉相亲”的紫荆花

我国香港特别行政区的区徽、区旗上都有一朵秀丽的紫荆花的图案。 紫荆花就是紫荆枝条上开的花,它的形态和色彩虽不像牡丹那样富丽,桃花那样娇艳,但它却同迎春花一样,是在早春时节,先于绿叶而绽放出一簇簇紫红色的花朵,昭示着阳春的来到。 紫荆,是遍布我国大江南北的一种花木,有野生的,有栽培的,在植物分类学上,属豆科植物,为落叶乔木,也有灌木,叶片呈圆心形,果实长而扁,荚内豆     

让生命的花蕾灿然绽放

在水潭旁边，是几株久未被人打扰的紫荆花。它们那细柔的枝条，在阳光下尽情舒展着，墨绿色的心形叶片，仍然透着盎然生机。     

紫荆花总黄酮对2型糖尿病模型大鼠的药效作用研究

目的:基于糖尿病并发症探讨紫荆花总黄酮对2型糖尿病模型大鼠的药效作用研究。方法:采用喂养高脂高糖饲料4周加一次性腹腔注射链脲菌素(65 mg·kg^-1)建立2型糖尿病大鼠模型,一周后测定空腹血糖值,选取血糖值在16.7~21.0 mmol·L^-1的大鼠,随机分为模型组、盐酸二甲双胍组(0.35 g·kg^-1)、紫荆花总黄酮(高、低剂量)(200 g·kg^-1、100 g·kg^-1)组,另设空白组,各组大鼠连续给予相应药物灌胃30 d。每2周测定空腹血糖值;腹主动脉取血,采用酶联免疫法测定血清糖化血清蛋白(glycated serum proteins,GSP)、胰岛素(insulin,INS)、胰岛素自身抗体(insulin autoantibody,IAA)、总胆固醇(total cholesterol,TC)、三酯甘油(triglyceride,TG)、高密度脂蛋白(high density lipoprotein,HDL-C)、低密度脂蛋白(low density lipoprotein,LDL-C)、总抗氧化能力(total antioxidant capacity,T-AOC)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)水平;全血检测血液流变学相关指标。结果:与模型组相比,紫荆花总黄酮组均可不同程度降低模型大鼠空腹血糖值;降低血清GSP、IAA、TC、TG、LDL-C、MDA水平,升高INS、HDL-C、SOD、T-AOC水平;对血液流变学相关指标也有很好的改善作用。结论:紫荆花总黄酮可有效调节2型糖尿病大鼠血清血脂水平紊乱,增强机体抗氧化能力,有效降低空腹血糖值,达到良好降糖效果。