Antitumor activity and antioxident role of Bauhinia racemosa against Ehrlich ascites carcinoma in Swiss albino mice

AIM: To study the enzyme activity of CYP2C18 variant with exon 5 skipped. METHODS: A full length CYP2C18 cDNA X1 and an exon 5 skipped variant CYP2C18 X2 were separately subcloned into mammalian expression vector pREP9 to transfect HepG2 cells. The expression of CYP2C18 mRNA in transgenic cells and human liver tissues were determined by RT-PCR. The enzyme activity of CYP2C18 to oxidate tolbutamide in postmitochondrial supernate (S9) fraction was determined by HPLC. The cytotoxicity of ifosfamide to transgenic cells was evaluated by MTT test. RESULTS: HepG2-CYP2C18 X1 cells showed strong expression of the full length CYP2C18 mRNA. On the other hand, HepG2-CYP2C18 X2 cells had only infinitesimal expression of the exon-skipped CYP2C18 as well as the full length CYP2C18, while non-transfected HepG2 cell only demonstrated an infinitesimal expression of the full length CYP2C18. The expression of CYP2C18 exons 2 to 7 was also analyzed by RT-PCR in 7 extratumoral liver tissues. Among them, 3 samples expressed on     

桂哌齐特治疗慢性脑供血不足80例

目的:探讨桂哌各特对慢性脑供血不足(CCCI)病人的临床疗效。方法:选择CCCI病人80例,给予桂哌齐特注射夜160mg,静脉滴注,疗程14d,观察疗效。并分别于治疗前后用TCD测量脑血流速度,阻力指数(RI),用比色法测定血浆丙二醛(MDA),超氧化物歧化酶(SOD),用放免法测定血浆内皮素(ET)、CGRP水平。结果:桂哌齐特治疗2周后,头晕、头重、失眠总有效率别为98.8%、93.2%、86.4%;血浆RI下降;血浆MDA和ET含量较治疗前下降(P<0.01),而SOD和CGRP水平较治疗前上升(P<0.01)。结论:桂哌齐治疗CCCI效果显著,无明显不良反应。     

硅胶管采样气相色谱法测定空气中乙醛丙醛

乙醛在国内尚未有监测检验的标准方法,一般常用3—甲基—2苯骈噻唑酮腙(酚试剂)比色法,此法易受其它醛类干扰。美国NIOSH法是用高压液相色谱法测定空气中的醛,但此仪器一时不能普及,不利用推广应用。本文采用色谱法具有灵敏度高,分离效果好的优点。同时利用固体吸附剂对有机蒸气的吸附效率高,稳定时间长,采样及携带方便等特点。试验选用硅胶管作吸附剂采样,气相色谱法测定空气中乙醛及丙醛。现将试验结果报告于后。     

脑出血大鼠血肿周围脑组织Mg~（2＋）水平、MDA含量与脑水肿的关系

目的：观察镁离子（Mg2＋）以及丙二醛（MDA）、在大鼠脑出血血肿周围脑组织中的表达。探讨Mg2＋及脂质氧化与脑水肿的关系。为丰富脑出血后脑水肿治疗奠定一定理论基础。方法：采用干湿重法、酶联免疫吸附法,火焰原子吸收法测50只脑出血模型组大鼠和50只假手术对照组大鼠血肿周围脑组织中含水量、Mg2＋以及MDA水平。结果：（1）脑出血组各时间点脑组织Mg2＋含量均低于对照组（P〈0.05）。脑出血组Mg2＋含量3d达最低,5d升高,5d与7d差别不显著。（2）脑出血组各时间点脑组织MDA含量均高于对照组（P〈0.05）。脑出血组MDA水平于3d达高峰,5d降低,5d与7d差别不显著。（3）脑出血组各时间点脑组织含水量均高于对照组（P〈0.05）。脑出血组脑组织含水量3d达高峰,5d下降,5d与7d差别不显著。结论：①大鼠脑出血血肿周围脑组织脂质氧化指标：MDA水平于血肿初期升高,3d达高峰,5d降低,5d与7d差别不显著。②大鼠脑出血血肿周围脑组织Mg2＋水平于血肿初期降低,3d达最低,5d升高,5d与7d差别不显著。③大鼠脑出血血肿周围脑组织Mg2＋水平与MDA及脑水肿程度呈负相关。     

一种新型聚乙二醇化重组人生长激素的制备及性质考察

目的制备一种新型聚乙二醇化重组人生长激素(PEG-(rhGH)2),并对其理化性质及药理作用进行研究。方法以分子质量为2×104u的聚乙二醇二丙醛(PEG-(ALD)2)对重组人生长激素(rhGH)进行修饰,采用Sephacryl S-200凝胶过滤色谱、Q Sepharose FF离子交换色谱对修饰产物进行分离纯化,SDS-PAGE鉴定各组分,通过SEC-HPLC、IEF、MALDI-TOF-MS等方法对目标产物进行分析,并采用去脑垂体大鼠模型研究其体内生物活性与长效作用。结果获得了一种新型聚乙二醇化重组人生长激素(PEG-(rhGH)2)。其纯度质量分数达到97%以上,等电点为5.27,分子质量为6.6×104u。与未修饰的rhGH相比,PEG-(rhGH)2具有高效、长效的促进机体生长的药理作用。结论新型聚乙二醇化重组人生长激素PEG-(rhGH)2是一种具有开发前景的长效蛋白质药物,值得进一步深入研究。     

Significance of malondialdehyde concentration in seminal plasma of infertile men

Objective: To determine the concentration of malondialdehyde (MDA), product of lipid peroxidation, in seminal plasma and evalu ate its significance. Methods: Ninety-three cases of infertile pa tients were divided into the obstructive azoospermic (12 cases), the non-obstructive azoospermic (15 cases), the oligozoospermic (21 cases), the asthenozoospermic (19 cases), the oligoastheno zoospermic (16 cases) and the oligoasthenoteratozoospermic (10) groups. Eighteen fertile males served as the controls. MDA con centration was assessed by high-performance liquid chromatogra phy (HPLC). Results: With the exception of the obstructive azoospermic group, the MDA concentration in the seminal plasma was significantly different between the control group and the infer tile groups (P<0.01) as well as between the different infertile groups. Conclusion: Determination of MDA concentration in the seminal plasma is helpful to the diagnosis of male infertility induced by overproduction of reactive oxygen species.     

卡维地洛对高血压病抗氧化活性的临床研究

目的:探讨卡维地洛对高血压(EH)病患者的抗氧化作用。方法:81例高血压病患者分为接受卡维地治疗洛组(n=42)和美托洛尔治疗组(n=39),在治疗前及治疗1、4、8、12周后检测oxLDL、SOD、MDA值。结果:卡维地洛组治疗12周后oxLDL、MDA均显著降低,SOD显著升高(P<0.01),并且这种趋势在治疗8周后已经体现,并维持至12周后。美托洛尔组较用药前oxLDL、SOD及MDA无显著性差异。结论:卡维地洛具有抗氧化作用,有可能通过oxLDL途径打断EH患者AS发生发展。     

酒精性肝病患者抗丙醛-乙醛加合物循环抗体滴度的测定

目的探讨丙醛-乙醛加合物（MAA）是否参与了酒精性肝病（ALD）患者的免疫反应。方法采用酶联免疫吸附试验（ELISA）检测40例酒精性肝病患者、40例非酒精性肝病患者、30例无肝损害的重度嗜酒者、40例健康对照的血清中MAA加合物的循环抗体,并评价MAA加合物的免疫反应。结果酒精性肝病患者抗MAAIgG滴度比健康对照组明显增加（平均OD值分别为0．42±0．23、0．10±0．04,P〈0．001）,非酒精性肝病及重度嗜酒者中抗HSA—MAAIgG抗体（平均OD值分别为0．14±0．08、0．13±0．08）与对照组相比没有显著性差异。在酒精性肝病患者中抗MAA抗体为阳性,而非酒精性肝病患者、重度嗜酒者和健康对照多数为阴性。结论MAA具有免疫原性,可引起肝脏自身免疫反应,导致肝损伤,是引起酒精性肝病的重要因素。     

Overweight and Obesity——Induced Oxidative Stress in Children

Objective To investigate whether overweight and obesity might cause oxidative stress and potential oxidative damage in overweight and obese children, and to explore its possible mechanism. Methods Eighty-five overweight and obese children （OOC）, and eighty-five age-matched healthy children （HC） were recruited in this case-control study. The present study analyzed spectrophotometrically vitamin C （VC）, vitamin E （VE）, and β-carotene （β-CAR） in plasma, as well as the activities of superoxide dismutase （SOD）, catalase （CAT）, and the level of malondialdehyde （MDA） in erythrocytes. Results Compared with those of VC, VE, β-CAR, SOD, CAT and MDA in the HC group, the average values of VC, VE, β-CAR, SOD, and CAT in the OOC group were significantly decreased （P〈0.001）, while the average value of MDA in the OOC group was significantly increased （P〈0.001）. The regression analysis demonstrated that VC, VE, β-CAR, SOD, and CAT were negatively correlated （P〈0.05-0.01）, and MDA was positively correlated with BMI （P〈0.05）. Fitting to the model of multiple stepwise regression of BMI on VC, VE, β-CAR, SOD, CAT, and MDA in 85 OOC was Y = 27.0041 ＋ 0,2541MDA - 2.1448β-CAR -- 0.0090CAr, where F = 43.8088, P〈0.001, r = 0.7866, r^2= 0.6187, adjusted r^2= 0.6046. The findings from the reliability analysis for VC, VE, β-CAR, SOD, CAT, and MDA used to reflect increased oxidative stress and potential oxidative damage in the OOC showed that the reliability coefficients （alpha, 6 items） = 0.7231, P〈0.0001, and that the standardized item alpha = 0.9207, P〈0.0001, Conclusion The present study suggests that there exists an increased oxidative stress in overweight and obese children.     

Serum malondialdehyde level in patients infected with Ascaris lumbricoides

AIM: The aim of the study was to investigate the changes of serum malondialdehyde level, i.e; the oxidative stress hypothesis in patients infected with Ascaris lumbricoides. METHODS: Serum malondialdehyde activity was measured in 43 patients who were positive for intestinal parasite of Ascaris lumbricoides. Scores were obtained for the positives and their age-and sex-matched 60 Ascaris lumbricoides negative healthy controls. RESULTS: The difference between malondialdehyde levels of patients infected with Ascaris lumbricoides and control group was statistically significant both for females (P<0.05) and for males (P<0.05). In the patient and control groups, no correlation was found between age and malondialdehyde levels (P>0.05) both in females and in males. In addition, no significant correlation could be found between malondialdehyde levels of both females and males for patients and control groups (P>0.05). CONCLUSION: Malondialdehyde levels clearly increase in patients infected with Ascaris lumbricoides.