生物羊膜覆盖治疗早期重度眼表烧伤

目的探讨生物羊膜在早期重度眼表烧伤中的临床疗效。方法对17例(20眼)重度眼表烧伤患者施行生物羊膜覆盖术。随访3~7月,观察临床疗效。结果术后全部患者均保存了眼球。角膜恢复透明11眼,角膜斑翳7眼,角膜白斑2眼,角膜新生血管9眼,睑球粘连1眼。12眼视力提高>3行。无急性排斥反应和继发感染发生。结论生物羊膜覆盖是治疗早期重度眼表烧伤一种安全有效的方法。     

大气中不同粒径颗粒物诱导人羊膜FL细胞UDS的研究

本研究以诱导人羊膜细胞ＵＤＳ为指标，对太原市大气不同粒径的颗粒物提取液进行了致突变性检验，结果表明，不同粒径颗粒物的提取液均可产生一定的遗传毒性，尤以３．３μｍ以下的颗粒物的遗传毒性较强。     

组织工程法修复兔肌腱缺损

目的　应用组织工程的原则 ,种植成纤维细胞于人羊膜细胞外基质 (HA ECM)以修复兔的肌腱缺损。方法　从胎兔分离的成纤维细胞用 5 溴脱氧尿嘧啶核苷 (BrdU)标记后种植于人羊膜细胞外基质上。以 1cm长的兔跟腱缺损为修复对象 ,用聚酯聚二氧杂环己烷缝线 (PDS)轴心桥接肌腱缺损 ,外包种植有成纤维细胞的HA·ECM膜。术后进行功能锻炼。结果　成纤维细胞在HA·ECM上生长良好 ,放射状或平行排列。成纤维细胞 HA ECM组能够修复肌腱缺损并且腱化的速度和质量明显优于仅用PDS和HA ECM(无成纤维细胞 )修复组 ,与正常肌腱相似 ,抗张强度达正常腱的 81 8%。BrdU免疫组化显示成纤维细胞在腱化过程中起了重要的作用。术后的功能锻炼促进了修复腱的腱化及抗张强度的提高。结论　成纤维细胞 HA·ECM膜能够有效修复肌腱缺损 ,可作为肌腱修复的另一种选择方法。     

快速培养纯化猪角朊细胞及复合羊膜体外培养实验研究

目的研究快速培养和纯化猪角朊细胞的方法及观察角朊细胞在脱细胞羊膜上的生长状况,为组织工程皮肤研究提供实验依据.方法采用加10%胎牛血清的人无血清角朊细胞培养基(defined keratinocyte-SFM,DKSFM)培养原代猪角朊细胞,分别用DKSFM(A组)、加5%胎牛血清的DKSFM(B组)、加10%胎牛血清的DKSFM(C组)培养传代猪角朊细胞,于接种后1、3、5和7 d观察猪角朊细胞的形态及生长曲线.利用猪角朊细胞与培养瓶黏附牢固的特点,用0.02%乙二胺四乙酸(ethylene diamine tetraacetic acid,EDTA)和0.05%胰蛋白酶分步消化,纯化细胞.将第2代猪角朊细胞接种在冻干脱细胞羊膜上,直接苏木素染色、石蜡切片、免疫组织化学染色等方法观察猪角朊细胞在脱细胞羊膜上的生长状况.结果采用加10%胎牛血清的DKSFM培养原代猪角朊细胞,细胞生长速度快,形态好,培养5 d铺满培养瓶底60%～70%.B组培养的传代猪角朊细胞生长速度较C组培养猪角朊细胞生长速度快.A组培养传代的角朊细胞,细胞生长缓慢.用0.02?TA和0.05%胰蛋白酶分步消化的方法纯化猪角朊细胞,可获得纯度为95%以上的猪角朊细胞.将第2代猪角朊细胞接种在脱细胞羊膜后12 d,可形成单层细胞,呈多角形、铺路石样排列;14 d和16 d细胞进一步密集,16 d细胞出现老化.结论 10%胎牛血清的DKSFM培养原代猪角朊细胞,5%胎牛血清的DKSFM培养传代猪角朊细胞,细胞生长速度快.用0.02?TA和0.05%胰蛋白酶分步消化的方法纯化猪角朊细胞,可以获得高纯度猪角朊细胞.脱细胞羊膜为猪角朊细胞提供良好的支架,接种培养后12 d,细胞形态最好.     

Extraembryonic motor activity during the embryogenesis of higher vertebrates

Extraembryonic rhythmic motor activity in higher vertebrates, along with the intrinsic motor activity of the embryo, is important for the normal development of the embryo. This can have different natures in different classes of amniotes (i.e., motor activities of the amnion, yolk sac, and uterus), but these have similar functional importance. This activity changes reproducibly during the process of embryogenesis, providing the optimum conditions for normal embryo development. During embryogenesis, a system for controlling extraembryonic rhythmic motor activity is also formed. There is a trend for the regulation of this activity to become more complex in mammals as compared with birds. Reptiles have received little study from this point of view. In addition to regular changes in extraembryonic rhythmic motor activity during embryogenesis which depend on the developmental stage of the embryo, motor activity can also change in response to changes in a number of environmental factors (for example, temperature and the gas composition of the air). This demonstrates the possible involvement of embryo-associated extraembryonic motor activity in adapting the embryo to changing environmental conditions and maintaining homeostasis for the development of the embryo itself. Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 84, No. 10, pp. 961–968, October, 1998.     

小梁切除联合羊膜移植治疗原发性青光眼临床研究

目的：观察小梁切除联合新鲜羊膜移植治疗原发性青光眼疗效评价。方法：34例48眼原发性闭角型青光眼：18例26眼原发性开角型青光眼施行小梁切除联合结膜瓣下及巩膜瓣下新鲜羊膜移植术,术后随访8－21个月,平均15．7个月。结果：49例71眼术后眼压均控制在正常范围内,眼压控制成功率95．95％,术后所有病例视力均有不同程度提高,视野损害均未见加深。结论：新鲜羊膜可有效防止滤过泡的疤痕组织形成,保持滤过通畅,并能长期有地保留功能性滤过泡。     

Placental tissue stem cells and their role in neonatal diseases

Neonatal diseases such as hypoxic ischemic encephalopathy, diseases of prematurity and congenital disorders carry increased morbidity and mortality. Despite technological advancements, their incidence remains largely unabated. Stem cell (SC) interventions are novel therapies in the neonatal world. In pre-clinical models of neonatal diseases, SC applications have shown encouraging results. SC sources vary, with the bone marrow being the most utilized. However, the ability to harvest bone marrow SCs from neonates is limited. Placental-tissue derived SCs (PTSCs), provide an alternative and highly attractive source. Human placentas, the cornerstone of fetal survival, are abundant with such cells. Comparing to adult pools, PTSCs exhibit increased potency, decreased immunogenicity and stronger anti-inflammatory effects. Several types of PTSCs have been identified, with mesenchymal stem cells being the most utilized population. This review will focus on PTSCs and their pre-clinical and clinical applications in neonatology.     

自体带结膜瓣角膜缘干细胞移植联合羊膜移植治疗复发性翼状胬肉

目的:降低翼状胬肉术后复发率,减少术后并发症。方法:采用自体带结膜瓣的角膜缘干细胞移植联合羊膜移植治疗26例(26眼)复发性翼状胬肉患者。结果:在26眼中显效25眼,复发1眼,有效率为96%。结论:采用自体带结膜瓣角膜缘干细胞移植联合羊膜移植,可有效维持角膜上皮与结膜上皮之间的屏障途径,阻止结膜上皮长入和新生血管形成,并使结膜下成纤维增殖抑制,睑球粘连松解,从而降低翼状胬肉术后复发率,减少术后并发症。     

羊膜联合自体角膜缘干细胞移植治疗翼状胬肉

目的探讨羊膜联合自体角膜缘干细胞移植治疗翼状胬肉的效果。方法采用羊膜联合自体角膜缘干细胞移植术治疗翼状胬肉56例(61眼)，随访平均12月。结果56例(61眼)术后均无复发，移植片存活，未见排斥反应发生，治愈率达100％。结论应用羊膜联合自体角膜缘干细胞移植治疗翼状胬肉，不仅取材方便、安全，而且可获得根治性治愈的理想效果，值得临床推广。