Pan-cancer proteomic map of 949 human cell lines

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Proteomic Profiling of Small Extracellular Vesicles Isolated from the Plasma of Vietnamese Patients with Non-Small Cell Lung Cancer Reveals Some Potential Biomarkers

Background: Considering the poor prognosis of non-small cell lung cancer (NSCLC), the objective of this study was to examine the potential of plasma-derived vesicles as a source of lung cancer-specific proteins. Extracellular vesicle (EV) cargos are specific to the source cells, hence they have the potential of being a source of cancer-specific proteins.  Methods: The proteins differently expressed in cancer were determined and derived from EVs isolated from the plasma of NSCLC patients at the National Lung Hospital. To this end, purification was done using gel filtration chromatography and ultracentrifugation. In addition, nano liquid chromatography mass spectrometry (LC–MS/MS) was used for analyzing. Results: Fifty-seven EV-derived proteins related to NSCLC were highlighted in this research. Some of them have not been addressed before, such as EEF1A1 (elongation factor 1-α1), KPNB1 (Importin subunit beta 1), SRC (proto-oncogene tyrosine-protein kinase) and ACTC1 (actin, alpha cardiac muscle 1). This list was further confirmed through a comparison with ExoCarta and Vesiclepedia. Conclusion: This study is the first work to show the involvement of several novel proteins of small EV (EEF1A1, KPNB1, SRC, and ACTC1) in the progression of NSCLC. The results suggested that they could serve as novel biomarkers for non-small cell lung cancer in the future.     

蛋白质组学及其在前列腺癌研究中的应用

随着蛋白质组学技术的发展,越来越多的肿瘤标记物被发现,并逐渐应用于临床,这将为肿瘤的早期诊断和预后监测提供可靠的依据。现综述蛋白质组学的研究策略及其在前列腺癌研究中的应用。     

慢性体外反搏对高胆固醇血症猪动脉内皮细胞的影响

目的 探讨慢性增强型体外反博（EECP）能否在体修复高胆同醇血症引起的猪动脉内皮细胞（ECs）损伤。方法 高胆固醇血症猪反搏36h后，取前降支进行扫捕电镜检查，收集胸主动脉ECs进行蛋白质组学检查。结果 高脂组冠脉ECs排列不规则、大量脱落、斑块形成，反搏组ECs呈流线型排列、脱落程度显著减轻：与高脂组相比，在反搏组的ECs检测到6种高表达的蛋白。结论 慢性EECP可能通过调节ECs蛋白表达，改善ECs粘附性和代谢及减轻ECs内皮凋亡，从而修复高胆固醇血症对动脉内皮形态和功能的损伤：     

眼蛋白质组学

蛋白质组学是对一个细胞、组织或器官的基因组表达的所有蛋白质分析研究，是新世纪生命科学研究的前沿以及功能基因组学的研究重心。蛋白质组学为研究眼部细胞生命活动规律和眼科疾病病变机制提供了一个新的研究平台。本文就蛋白质组学在眼科的应用作一综述。眼科学报2003；19：67-70     

Capacitation-dependent concentration of lipid rafts in the apical ridge head area of porcine sperm cells

Lipid architecture of the plasma membrane plays an important role in the capacitation process of the sperm cell. During this process, an increase in membrane fluidity takes place, which coincides with a redistribution of cholesterol to the apical region of the head plasma membrane and subsequently an efflux of cholesterol. Cholesterol is also a major player in the formation of lipid rafts or microdomains in the membrane. Lipid rafts favour specific protein-protein interactions by concentrating certain proteins in these microdomains while excluding others. In this study, we investigated the organization of lipid rafts during in vitro capacitation of boar sperm cells. We report on the presence of the lipid raft-specific proteins caveolin-1 and flotillin-1 in sperm cells. Capacitation induced a change in membrane distribution of these proteins. Lipid analysis on detergent-resistant membranes (DRMs) of sperm cells indicated that capacitation induces a lipid raft concentration rather than a disintegration of lipid rafts, because the total amount of lipid in the DRM fraction remained unaltered. Using a proteomic approach, we identified several major DRM proteins, including proteins involved in capacitation-dependent processes and zona pellucida binding. Our data indicate that sperm raft reorganization may facilitate capacitation-specific signalling events and binding to the zona pellucida.     

Mild atopic dermatitis lacks systemic inflammation and shows reduced nonlesional skin abnormalities

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Immuno-proteomic profiling reveals aberrant immune cell regulation in the airways of individuals with ongoing post-COVID-19 respiratory disease

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蛋白质组学在心血管研究中的应用

随着基因组数据库的不断完善和蛋白质组学技术的不断发展，已有越来越多的研究者将蛋白质组学运用到心血管疾病研究中，即运用2—DE、质谱等手段来观察心肌缺血、高血压、心肌肥大、心衰、心肌梗塞导致的心肌细胞蛋白质表达水平和翻译后修饰(PTM)的改变，对这些急慢性心血管疾病的分子机制给予独特的解释。与此同时，各种心血管疾病模型相关蛋白质组数据库也在建设之中，这将大大方便更多的蛋白质组研究。本文综述了近年来使用蛋白质组学在心血管研究中所取得的进展。