Trigonella foenum graecum (fenugreek) seed extract as an antineoplastic agent

The antineoplastic effect of Trigonella foenum graecum seed extract has been evaluated in the Ehrlich ascites carcinoma (EAC) model in Balb-C mice. Intra-peritoneal administration of the alcohol extract of the seed both before and after inoculation of EAC cell in mice produced more than 70% inhibition of tumour cell growth with respect to the control. Treatment with the extract was found to enhance both the peritoneal exudate cell and macrophage cell counts. The extract also produced a significant antiinflammatory effect. We report here the antiinflammatory and antineoplastic effects, of Trigonella foenum graecum seed extract.     

Physiological Aspects of Male Libido Enhanced by Standardized Trigonella foenum‐graecum Extract and Mineral Formulation

The aim of the clinical study was to evaluate the effect of Testofen, a standardized Trigonella foenum‐graecum (Fenugreek) extract and mineral formulation, on male libido (sexual drive, urge or desire) in a double blind randomized placebo controlled study. The study recruited 60 healthy males aged between 25 and 52, without erectile dysfunction and randomized to an oral dose (two tablets per day) of the active treatment (600 mg Testofen per day) or placebo for 6 weeks. The primary outcome measure was the DISF‐SR (male) self‐administered QOL total score and the four domain scores. The secondary outcome was specific quality of life parameters. Testofen had an overall positive effect on physiological aspects of libido. In particular, there was a significant increase in the subdomains of sexual arousal and orgasm. Testofen had a positive effect on QOL in self‐reported satisfaction with muscle strength, energy and well‐being but did not have an effect on mood or sleep. Serum prolactin and testosterone levels remained within the reference range. It was concluded that Testofen demonstrated a significant positive effect on physiological aspects of libido and may assist to maintain normal healthy testosterone levels. Copyright © 2011 John Wiley & Sons, Ltd.     

Efficacy of a Proprietary Trigonella foenum‐graecum L. De‐Husked Seed Extract in Reducing Menopausal Symptoms in Otherwise Healthy Women: A Double‐Blind,Randomized, Placebo‐Controlled Study

Trigonella foenum‐graecum seed extract has demonstrated hormone modulatory activity, providing biological plausibility for relieving menopausal symptoms. The study aimed to assess efficacy of a standardized T. foenum‐graecum de‐husked seed extract in reducing menopausal symptoms in healthy aging women. The study was a double‐blind, randomized, placebo‐controlled trial that recruited 115 women aged 40 to 65 years of which 59 were allocated to active (n = 54 completed) and 56 to placebo (n = 50 completed). Active treatment was T. foenum‐graecum de‐husked seed extract, 600 mg per day for 12 weeks. Outcome measures included Menopause‐Specific Quality of Life (MENQOL) questionnaire, frequency of hot flushes and night sweats and serum estradiol levels. There was a significant reduction in menopausal symptoms in the active group compared with placebo as assessed by total MENQOL score (p < 0.001); reflected by significant improvements in the vasomotor (p < 0.001), psychosocial (p < 0.001), physical (p < 0.001) and sexual symptoms (p < 0.001) domains. Vasomotor outcomes correlated with hot flushes, the active group reporting significantly less daytime hot flushes and night sweats at 12 weeks (p < 0.001). The average estradiol levels were similar in both the active group and placebo group after treatment. This study demonstrated that this proprietary T. foenum‐graecum de‐husked seed extract may reduce menopausal symptoms in healthy women. Copyright © 2017 John Wiley & Sons, Ltd.     

胡芦巴对急性化学性肝损伤的保护作用

目的 探讨胡芦巴对急性化学性肝损伤的保护作用及其机理。方法 采用四氯化碳和Ｄ－氨基半乳糖所致小鼠急性肝损伤的模型,染毒前不同时间、不同剂量给药对小鼠肝功能指标ＡＬＴ和ＡＳＴ的影响,同时观察脂质过氧化水平。结果 胡芦巴有效地抑制ＣＣＬ４和Ｄ－氨基半乳糖所致小鼠急性肝损伤的血清ＡＬＴ和ＡＳＴ的升高,并呈现良好的剂量效应关系;不同治疗时间、每日给药次数对疗效有很大影响,每日３次给药,治疗３天以上效果较好     

Influence of a Specialized Trigonella foenum‐graecum Seed Extract (Libifem), on Testosterone,Estradiol and Sexual Function in Healthy Menstruating Women,a Randomised Placebo Controlled Study

The aim of the study was to evaluate the effect of Trigonella foenum‐graecum (fenugreek) seed extract on sex hormones and sexual function in healthy menstruating women who reported low sexual drive. This short term, single site, double blind, randomised, placebo‐controlled study was conducted on 80 women, aged 20 to 49 years. Participants were randomised to either an oral dose of a standardised T. foenum‐graecum seed extract (libifem) at a dose of 600 mg/day or placebo over two menstrual cycles. Dehydroepiandrosterone sulfate, progesterone, androstenedione, total and free testosterone, estradiol (E2), luteinizing hormone, follicle stimulating hormone, sex hormone binding globulin and cholesterol were measured at baseline and 8 weeks. The individual aspects of sexual function were measured using the Derogatis interview for sexual functioning and female sexual function index self‐administered questionnaires. Stress, fatigue and quality of the relationship with partner were also measured using the PSS (Perceived Stress Scale), MFI‐20 (Multidimensional Fatigue Inventory) and DAS (Dyadic Adjustment Scale) quality of life measures, respectively. There was a significant increase in free testosterone and E2 in the active group as well as sexual desire and arousal compared with the placebo group. The results indicate that this extract of T. foenum‐graecum may be a useful treatment for increasing sexual arousal and desire in women. Copyright © 2015 John Wiley & Sons, Ltd.     

Mitigation of thyroxine-induced hyperglycaemia by two plant extracts

Extracts of Trigonella foenum-graecum (TFG) seed and Allium sativum (AS) bulb were evaluated for their efficacy to ameliorate l-thyroxine (l-T4) induced hyperglycaemia in rats. Simultaneously, the serum cholesterol concentration, a supporting parameter for thyroid function, was also estimated. Thyroxine treatment in rats (300 microg/kg b. wt./day) increased the levels of both the thyroid hormones, namely thyroxine (T4) and tri-iodothyronine (T3) with a concomitant elevation in serum glucose concentration and a reduction in serum cholesterol level. Administration of TFG (220 mg/kg/day) and AS (500 mg/kg/day) extracts in hyperthyroid animals decreased the serum glucose concentration as well as the serum thyroid hormones. For comparison, propyl thiouracil (PTU), an antithyroid compound, was used as the standard at a daily dose of 10 mg/kg. The reductions in serum glucose and thyroid hormone concentrations in the plant extract treated groups were comparable to that in PTU treated animals. Our findings indicate that TFG seed and AS bulb extracts may prove to be effective in the treatment of thyroxine-induced hyperglycaemia.     

胡芦巴中皂苷成分的研究 Ⅱ.新皂苷B和C的分离纯化及化学结构测定

目的 对胡芦巴Trigonella foenum-graecum种子所含的皂苷成分作深入研究。方法 采用硅胶H柱色谱，干柱色谱等方法从总皂苷中分离纯化皂苷成分并通过^13C—NMR，FAB—MS，DEPT等波谱法及部分水解获得次生苷的方法测定了化学结构。结果 从总皂苷中分出两个新皂苷B和C，均为薯蓣皂苷元的四糖苷。经测定皂苷B的化学结构为：薯蓣皂苷元—3—O—α—L—鼠李吡喃糖(1→3)—α—L—鼠李吡喃糖(1→4)—β—D—葡萄吡喃糖(1→4)—β—D—葡萄吡喃糖苷；皂苷C的结构为薯蓣皂苷元—3—O—β—D—葡萄吡喃吡喃糖(1→4)—α—L—鼠李吡喃糖(1→4)—β—D—葡萄吡喃糖(1→4)—β—D—葡萄吡喃糖苷。结论 皂苷B和C是两个新的四糖皂苷。     

胡芦巴中皂苷成分的研究.新皂苷A及其次生苷的分离纯化和化学结构测定

目的　对胡芦巴 Trigonella foenum-graecum种子所含的皂苷成分作深入研究。方法　采用大孔吸附树脂法提取纯化总皂苷 ,并应用硅胶 H柱层析、干柱层析等方法分离出单一皂苷成分 ,通过 1 3 CNMR,FAB-MS,DEPT等光谱及部分水解获得的次生苷的方法进行了结构测定。结果　从总皂苷中分出一个新皂苷 A,为测定其结构将其部分水解获得次生苷 , 。经测定皂苷 A的化学结构为薯蓣皂苷元 -3 -O-α-L -鼠李吡喃糖 (1→ 4) -β-D-葡萄吡喃糖 (1→ 4) -β-D-葡萄吡喃糖苷 ;次生苷 结构为 :薯蓣皂苷元 -3 -O-β-D-葡萄吡喃糖苷 ;次生苷 结构为薯蓣皂苷元 -3-O-β-D-葡萄吡喃糖 (1→ 4) -β-D-葡萄吡喃糖苷。结论　皂苷 A为一新的三糖皂苷     

胡芦巴中皂苷成分的研究Ⅰ.新皂苷A及其次生苷的分离纯化和化学结构测定

目的　对胡芦巴 Trigonella foenum-graecum种子所含的皂苷成分作深入研究。方法　采用大孔吸附树脂法提取纯化总皂苷 ,并应用硅胶 H柱层析、干柱层析等方法分离出单一皂苷成分 ,通过 1 3 CNMR,FAB-MS,DEPT等光谱及部分水解获得的次生苷的方法进行了结构测定。结果　从总皂苷中分出一个新皂苷 A,为测定其结构将其部分水解获得次生苷 , 。经测定皂苷 A的化学结构为薯蓣皂苷元 -3 -O-α-L -鼠李吡喃糖 (1→ 4) -β-D-葡萄吡喃糖 (1→ 4) -β-D-葡萄吡喃糖苷 ;次生苷 结构为 :薯蓣皂苷元 -3 -O-β-D-葡萄吡喃糖苷 ;次生苷 结构为薯蓣皂苷元 -3-O-β-D-葡萄吡喃糖 (1→ 4) -β-D-葡萄吡喃糖苷。结论　皂苷 A为一新的三糖皂苷     

胡芦巴中皂苷成分的研究Ⅱ.新皂苷B和C的分离纯化及化学结构测定

目的 对胡芦巴Trigonella foenum-graecum种子所含的皂苷成分作深入研究。方法 采用硅胶H柱色谱，干柱色谱等方法从总皂苷中分离纯化皂苷成分并通过^13C—NMR，FAB—MS，DEPT等波谱法及部分水解获得次生苷的方法测定了化学结构。结果 从总皂苷中分出两个新皂苷B和C，均为薯蓣皂苷元的四糖苷。经测定皂苷B的化学结构为：薯蓣皂苷元—3—O—α—L—鼠李吡喃糖(1→3)—α—L—鼠李吡喃糖(1→4)—β—D—葡萄吡喃糖(1→4)—β—D—葡萄吡喃糖苷；皂苷C的结构为薯蓣皂苷元—3—O—β—D—葡萄吡喃吡喃糖(1→4)—α—L—鼠李吡喃糖(1→4)—β—D—葡萄吡喃糖(1→4)—β—D—葡萄吡喃糖苷。结论 皂苷B和C是两个新的四糖皂苷。