Improved haemorheology associated with a reduction in plasma fibrinogen and LDL in patients being treated by heparin-induced extracorporeal LDL precipitation (HELP)

Heparin-induced Extracorporeal LDL-Precipitation (HELP) is an effective procedure for the elimination of both plasma LDL and fibrinogen. In 10 adult patients with severe type II hyperlipoproteinemia, a single HELP treatment of 3 1 plasma led to an acute decrease in the average plasma viscosity (PV) from 1.30 to 1.1 mPas. At the same time, an even more marked decrease in the mean erythrocyte aggregation rate from a pathological value of 7.9% to a value of 3.7% (normal range less than 5%) was observed. Long-term studies on five patients demonstrated a lasting improvement in these two haemorheological variables. The acute rheological changes were also accompanied by an improvement in polarographically determined muscle oxygen tension. Mean oxygen tension values measured in both the m.biceps brachii and the m.tibialis anterior in five patients before and after a single HELP treatment increased from 30 +/- 4 to 37 +/- 7 mmHg and from 27 +/- 2 to 31 +/- 3 mmHg respectively. These results may provide an explanation for the rapid improvement in patients' clinical symptoms such as angina pectoris and in stress electrocardiogram which have been observed during HELP therapy.     

老年心力衰竭患者外周血红细胞膜脂流动性的变化及与心功能的相关性

目的观察红细胞膜脂流动性（LFU）在老年充血性心力衰竭（CHF）中的变化及其意义。方法选择CHF患者40例,正常对照者30例,用荧光分光光度计偏振技术测定外周血红细胞LFU。结果CHF组红细胞LFU明显低于正常对照组（P<0.01）;心功能越差,LFU越低（P<0.05和P<0.01）。结论红细胞LFU在一定程度上反映了CHF程度。     

晕可消胶囊治疗椎—基底动脉缺血性眩晕疗效观察及其对红细胞膜流动性的影响

目的:较大样本观察晕可消胶囊治疗椎-基底动脉缺血性眩晕疗效及其对红细胞膜流动性的影响。方法:符合入选标准的门诊病例203例随机分为观察组(晕可消组)102例,对照组(维脑路通组)101例,治疗10d后行疗效评定并以荧光偏振法检测红细胞膜流动性的变化。结果:观察组疗效优于对照组,并显著改善了红细胞膜的流动性。结论:晕可消胶囊具有改善红细胞膜流动性作用,用于治疗椎-基底动脉缺血性眩晕疗效肯定,值得推广。     

Effect of “Re Du Qing” on the activation,proliferation and membrane fluidity of lymphocytes

Summary Effects of Chinese Medicinal Preparation “Re Du Qing” (RDQ) on the activation, proliferation and membrane fluidity of T lymphocytes from human peripheral blood Were studied by means of³H-TdR incorporation and DPH fluorescence polarization. The results showed that “RDQ” can:1) significantly inhibit the activation of T lymphocytes; 2) restrain the proliferation of activated T lymphoblasts in the presence of exogenous interleukin-2 (IL-2); and 3) increase the membrane fluidity of T lymphocytes and antagonize the decreased fluidity of lymphocyte membrane mediated by Con A or PHA. The functional abnormalities of T lymphocytes in some autoimmune diseases such as arthritis and the usefulness of RDQ in the treatment of these diseases were also discussed.     

Studies on monoclonal anti-isotypic and anti-idiotypic antibodies against leukemia and myeloma: IV modulation of membrane fluidity of leukemic cell lines and tonsillar cell stimulated with McAbs

Summary In this study the technique of labelling the cell membrane with DPH fluorescence polarization was used to observe the membrane fluidity of B lymphocytic cell lines and tonsillar cells from healthy persons; the modulation effect on membrane-fluidity induced by McAbs against isotypic and idiotypic determinants of IgM from patients with leukemia was studied as well. The expression of the corresponding isotypic and idiotypic determinants of IgM on the cell membrane was determined. The results show that the membrane fluidity of leukemic cell lines is remarkably higher than that of tonsillar cells from healthy persons, and McAbs against isotypic determinants of leukemic IgM can enhance the membrane fluidity of all kinds of cells mentioned above. However, the anti-idiotypic monoclonal antibody increased only the membrane fluidity of leukemic cell lines. These results indicated that there was a close relationship between the effect of McAbs on cell membrane fluidity and the expression of corresponding isotypic and idiotypic determinants of IgM on the cell membrane.     

烧伤后淋巴细胞膜流动性变化

报告以ＤＰＨ作为膜标记探剂，用荧光偏振法测定烧伤后淋巴细胞膜流动性变化。将小鼠背部造成１１％～１２％体表面积Ⅲ度烧伤，于伤后第６天取脾淋巴细胞，测其膜流动性。结果表明：烧伤后淋巴细胞膜流动性明显下降（Ｐ＜０．００１），同时淋巴细胞增殖反应及ＣｏｎＡ刺激的ＩＬ－２生成亦下降；淋巴细胞功能受抑制的程度与淋巴细胞膜流动性呈负相关；脾淋巴细胞膜流动性与脾组织过氧化脂质水平呈负相关。结果提示烧伤后淋巴细胞膜流动性下降可能是烧伤后淋巴细胞功能抑制的原因之一，膜流动性降低与脂质过氧化亢进有关     

板蓝根磷脂对内毒素血症小鼠巨噬细胞膜脂流动性的影响

目的　以小鼠内毒素血症为模型 ,观察板蓝根磷脂对内毒素血症小鼠巨噬细胞膜脂流动性的保护作用。方法　小鼠分为板蓝根氯仿提取物预处理组、磷脂脂质体预处理组、板蓝根磷脂脂质体预处理组和内毒素血症对照组。各组按照上述次序分别给予腹腔注射 5ml/kg相应药物 ,预处理 18h后腹腔注射内毒素 6mg/kg。 6h后处死小鼠 ,观察细胞膜脂流动性的变化。结果　板蓝根氯仿提取物对内毒素血症小鼠巨噬细胞膜脂流动性的保护作用没有达到具有统计学意义的程度 (P >0 .0 5 ) ,磷脂脂质体对膜脂流动性具有保护作用 (P <0 .0 5 ) ,但两者之间并没有统计学上的差别 (P >0 .0 5 ) ;板蓝根磷脂对内毒素血症小鼠细胞膜脂流动性具有明显的保护作用 (P <0 .0 1) ,优于单独使用板蓝根氯仿提取物 (P <0 .0 5 )或磷脂脂质体 (P =0 .0 5 )。结论　板蓝根磷脂脂质体对内毒素血症小鼠巨噬细胞膜脂流动性的保护作用优于单独使用板蓝根氯仿提取物或磷脂脂质体     

用荧光探剂DPH研究家蝇线粒体膜脂流动性

本文探讨了用ＤＰＨ作为荧光探剂，研究家蝇飞翔肌线粒体膜脂流动性的方法。实验表明：ＤＰＨ确是用来研究家蝇生物膜流动性的有效探剂。同时，初步观察了不同杀虫剂对ＤＰＨ与线粒体膜结合后荧光强度的影响。其中氯菊酯和倍硫磷、马拉硫磷、敌百虫、氧化乐果，可使ＤＰＨ探剂与线粒体膜结合后的荧光相对强度增加；而氰戊菊酯、氯氰菊酯，溴氰菊酯及辛硫磷、乙酰甲胺磷，则可使荧光强度减弱。     

慢性肺心病红细胞变形性与红细胞膜流动性变化（附17例病例分析）

用荧光偏振法测定17例慢性肺心病急性发作期患者及17名健康人红细胞膜流动性,用微孔泸膜法测定其中10例红细胞变形性。结果,肺心病患者及正常人红细胞膜微粘度分别为4.64±1.56及3.51±1.05η(p<0.05);红细胞滤速分别为384±136及233±97(p<0.01)。红细胞滤速与红细胞膜粘度间呈明显正相关(r=0.802,p<0.01)。结果提示,肺心病患者红细胞膜流动性下降可能对红细胞变形性下降起一定作用。