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Teruyuki Usuba Yutaka Suzuki Akira Kuramochi Hisao Tajiri Katsuhiko Yanaga 《Digestive endoscopy》2007,19(1):18-21
Background: Buried bumper syndrome (BBS) is a rare complication of percutaneous endoscopic gastrostomy (PEG). Along with the widespread use of the button‐type kit, BBS is encountered frequently. Methods: In the present study, we examined causes and treatments for BBS among 1400 patients who had undergone PEG. Results: The causes of BBS after PEG were classified into two categories: early causes consisted of wound infection, inappropriate size of kit and severe lordosis, while late causes were inappropriate exchange of kit, rough management or weight gain. The treatments for BBS could be determined by the degree of wound infection, fistula and burial of the bumper. Conclusion: We prepared a flowchart for replacement, by which BBS can be managed safely and quickly without surgical or endoscopic intervention. 相似文献
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Kazumasa Miyake Atsushi Tatsuguchi Mikiko Tachibana Masanobu Kusunoki Yoko Shinji Kei Shinoki Tetsuro Hiratsuka Kazuhiro Nagata Hitoshi Nishigaki Seiji Futagami Ken Wada Taku Tsukui Toshiro Yoshiyuki Akira Tokunaga Takashi Tajiri Choitsu Sakamoto 《Digestive endoscopy》2004,16(2):172-175
A 52‐year‐old Japanese woman who presented with gastrointestinal (GI) bleeding underwent a proximal gastrectomy for a gastrointestinal stromal tumor (GIST) with a foveolar hyperplasia at the apex of the tumor, 4.5 cm in size, located in the upper body of the stomach. Although GIST are often asymptomatic and are found only incidentally, clinical symptoms such as bleeding, abdominal pain, or obstruction, occasionally lead to a premorbid diagnosis. When submucosal tumors present GI bleeding, the source of the bleeding usually is an ulceration of the mucosa over the tumor. However, in the present study, it was thought that the bleeding originated from the region of foveolar hyperplasia. 相似文献
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血清铁快速灵敏检测试剂盒的研制 总被引:2,自引:1,他引:1
血清铁试剂盒测定36例血清铁含量,平均值为18.4μmol/L,用亚铁嗪法测定,平均值为18.5μmol/L,差异无显著意义(P>0.05)。该法的工作曲线范围为0~71.6μmol/L,回收率98.6~102.6%,批内、批间精密度分别小于2.23%和2.80%。与亚铁嗪法相比,相关性很好(r=0.9957)。结果表明,本试剂盒方法具有灵敏、准确、快速、简便、血清耗量少等优点。 相似文献
5.
《Journal of labelled compounds & radiopharmaceuticals》2004,47(8):477-483
The synthesis of a novel technetium‐99m labelled derivative of L ‐tyrosine as a potential tumour imaging agent for nuclear medicine diagnosis is reported. The synthesis involved the labelling precursor fac‐[99mTc(OH2)(CO)3]+ which was synthesized using the commercially available Isolink®‐labelling kit and the tyrosine derivative O‐(N,N‐bis(carboxymethyl)aminoethyl)‐L ‐tyrosine trifluoroacetate. The labelled compound O‐(99mTc(I)‐tricarbonyl‐N,N‐bis(carboxymethyl)aminoethyl)‐L ‐tyrosine was obtained in a radiochemical yield of 70–80% within 60 min with a radiochemical purity greater than 98% without any HPLC purification step. Purification was achieved merely by solid phase extraction. Chemical as well as chiral purity was determined using gradient‐ and chiral HPLC. Copyright © 2004 John Wiley & Sons, Ltd. 相似文献
6.
c-kit与p27蛋白在胃肠道间质瘤诊治中的临床意义 总被引:2,自引:0,他引:2
目的研究c-kit和p27蛋白在胃肠道间质瘤中的表达及其与间质瘤临床病理特征的关系,为GIST诊断、预后评估提供客观指标。方法收集54例手术切除的胃肠道间质瘤标本,同时取距瘤灶>5cm的正常组织,应用免疫组织化学S-P检测肿瘤组织及正常组织中c-kit和p27蛋白的表达。结果54例胃肠道间质瘤中,c-kit和p27蛋白阳性表达率分别为94.44%和59.26%。c-kit蛋白表达水平在GIST不同分化程度、有无复发及远处转移的差异无显著性意义(P>0.05),p27蛋白表达水平在GIST不同分化程度、有无局部复发间差异有显著性(P<0.01)。结论c-kit作为特异敏感的标志物,对GIST的诊断及鉴别诊断有重要意义,而检测p27蛋白可作为评估GIST恶性程度和判断预后的重要指标。 相似文献
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冬虫夏草为我国传统名贵中药材。资源短缺、价格昂贵和巨大的市场需求导致市售冬虫夏草掺伪掺假现象严重,亟需建立快速有效的鉴别方法。该研究基于实验前期已建立的冬虫夏草快速鉴定SS-PCR方法,按照体外诊断试剂评价标准开发试剂盒,并对试剂盒的特异性、检测限、重复性及保存期4项性能进行了评价。结果显示冬虫夏草质量占混合物质量的1/200(质量分数0.5%以上)以上时可被成功检测。试剂盒对冬虫夏草及其伪品的检测结果显示,仅有冬虫夏草可被成功扩增,并在紫外下显示绿色荧光。该试剂盒37℃温育7 d仍有效,说明4℃保存其在1年内稳定有效。同一批次试剂盒在不同条件下,不同批次试剂盒在相同条件下,其检测结果均一致且准确,表明试剂盒具有良好的批内及批间重复性。将该试剂盒用于市售冬虫夏草及其混伪品的检测,操作简单快速,结果准确可靠,为下一步中药快检试剂盒的商品化奠定了基础。 相似文献
9.
Leandro E. Mainetti Xiaoning Zhe Jonathan Diedrich Allen D. Saliganan Won Jin Cho Michael L. Cher Elisabeth Heath Rafael Fridman Hyeong‐Reh Choi Kim R. Daniel Bonfil 《International journal of cancer. Journal international du cancer》2015,136(1):11-20
Loss of BRCA2 function stimulates prostate cancer (PCa) cell invasion and is associated with more aggressive and metastatic tumors in PCa patients. Concurrently, the receptor tyrosine kinase c‐kit is highly expressed in skeletal metastases of PCa patients and induced in PCa cells placed into the bone microenvironment in experimental models. However, the precise requirement of c‐kit for intraosseous growth of PCa and its relation to BRCA2 expression remain unexplored. Here, we show that c‐kit expression promotes migration and invasion of PCa cells. Alongside, we found that c‐kit expression in PCa cells parallels BRCA2 downregulation. Gene rescue experiments with human BRCA2 transgene in c‐kit‐transfected PCa cells resulted in reduction of c‐kit protein expression and migration and invasion, suggesting a functional significance of BRCA2 downregulation by c‐kit. The inverse association between c‐kit and BRCA2 gene expressions in PCa cells was confirmed using laser capture microdissection in experimental intraosseous tumors and bone metastases of PCa patients. Inhibition of bone‐induced c‐kit expression in PCa cells transduced with lentiviral short hairpin RNA reduced intraosseous tumor incidence and growth. Overall, our results provide evidence of a novel pathway that links bone‐induced c‐kit expression in PCa cells to BRCA2 downregulation and supports bone metastasis. 相似文献
10.