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1.
3种霍乱弧菌检测方法在外环境标本中的应用研究 总被引:2,自引:1,他引:1
目的:通过改进霍乱弧菌检测的技术手段,提高外环境标本中霍乱弧菌的检出率。方法:将常规分离鉴定法、PCR检测法和胶体金免疫层析试验法同时用于检测霍乱流行期间的外环境标本,并对其现场应用结果进行比较分析。结果:3种方法检测80份外环境标本霍乱弧菌的结果显示:它们均未出现假阳性,3种方法各有优势,且结果相互符合性好,但也存在各自弱点。结论:只用单一种方法来检测外环境标本霍乱弧菌存在明显不足。建议在外环境霍乱监测中选用胶体金法进行初筛,再用常规法和PCR法检测。最大限度减少漏检的可能性。 相似文献
2.
本研究主要对El Tor型霍乱弧菌(Vibrio cholerae biotvpe eltor,EVC)L型的某些生物学特性如生化反应、抗生素敏感性及超微结构等进行了探讨.研究发现EVC不稳定L型的生化性状与EVC流行株的非常相似,而稳定L型则变化很大.抗生素敏感性试验显示EVC稳定L型对某些作用于细胞壁的抗生素的敏感性减弱,而对大多数作用于蛋白质合成的抗生素的敏感性增强.与流行株相比,EVC稳定L型和不稳定L型的超微结构的改变很明显.此外,本文还讨论了产生这些改变的可能机制. 相似文献
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对不同来源、不同血清型的71株副溶血弧菌染色体DNA指纹图及其质粒图谱进行了分析研究。结果表明:不同血清型、不同来源菌株的DNA指纹图有显著的不同,相同血清型而来源不同的菌株其DNA指纹图亦有区别,来自同一起暴发的同一克隆菌株的DNA指纹图则极为相似,而且该DNA指纹图具有极好的稳定性和精确性。质粒分析显示,其检出率为87.32%,且图谱呈多样性,但未发现质粒分布与血清型及来源的相关性。 相似文献
5.
目的:为寻求肠道G杆菌及弧菌快速鉴定的方法。方法:菌种经增菌及分离培养后,取菌落接种于综合生化培养基,并在综合生化培养基管口悬挂硫化氢和靛基质试纸条。同时以克氏双糖铁培养基作对照,置37℃培养18-24h。取综合生化管培养基,外加测试氧化酶,共获取11项生化指标。结果:对689株不同菌种与综合生化管培养基和常规双糖铁培养的测试结果,符合率为99.97%,(7577/7579)和99.79%(7563/7579)。结论:综合生化管是适合医疗卫生单位微生物实验室的一种快速检验方法。 相似文献
6.
QING HUA ZOU BO QING LI JIAN ZHONG ZHANGNational Institute for Communicable Disease Control Prevention Chinese Center for Disease Control Prevention Beijing P.R.China 《中华微生物学和免疫学杂志(英文版)》2005,3(2):83-88
Cholera ,as one of the most severe epidemic dis-eases inthe world,can occurin a short time ,andspread quicklyto a wide region.Until 1992 ,onlyV.choleraeserogroup O1 was recognized as thecause of epidemic cholera . However in October1992 ,a major outbreak of a cholera-like illnesscaused by a newserogroupstrain ofV.choleraee-mergedinIndia and Bangladesh.In contrast to allpreviously reported non-O1 strains , which induceonly sporadic cases of human diarrhea without epi-demic potential ,the new… 相似文献
7.
Vibrio parahaemolyticus, a universal marine pathogen with available genome sequences, could be used as a bacterial model to clarify the various physiological phenomena of its native and host environments. In the present study, proteomic methodologies were applied to investigate the expression pattern of outer membrane proteins (OMPs) of V. parahaemolyticus at different NaCl concentrations. OmpW, OmpV, elongation factor TU and polar flagellin were determined to be osmoregulation-sensitive OMPs, among which OmpW and OmpV were reported to vary with changed NaCl concentrations in the pattern of osmolarity regulation. Therefore, our results not only expand our knowledge on osmoregulation-related proteins, but also provide a valuable strategy for the screening of salt-sensitive proteins. 相似文献
8.
Kanagawa phenomenon-positive strains of Vibrio parahaemolyticus contain two copies of the tdh gene (tdh1 and tdh2) encoding thermostable direct hemolysin (TDH). Previous studies suggested that the tdh2 gene, but not the tdh1 gene, was responsible for production of extracellular TDH. In this study, a tdh2-deficient isogenic mutant of Kanagawa phenomenon-positive strain AQ3815 was constructed by a suicide vector-mediated in vivo recombination method. The intact tdh1 gene in the mutant contributed little to Kanagawa phenomenon on Wagatsuma agar but produced TDH in broth media, accounting for 0.5–9.4% of total extracellular TDH of AQ3815. 相似文献
9.
The effect of various immunomodulators on the induction of experimental autoimmune encephalomyelitis (EAE) is evaluated in the Lewis rat. Bordetella pertussis (BP) is the optimal inductor of EAE in this rat strain. Treatment of the animals with BP either before or after or simultaneously with guinea-pig spinal cord preparation (GpSC) resulted in an EAE about two weeks thereafter. Additional injection of living BCG, of CFA, IFA (incomplete Freund's adjuvant) or Vibrio cholerae neuraminidase (VCN) did not augment or mitigate the effect induced by BP or GpSC. Living BCG, IFA, VCN or Corynebacterium parvum (CP) did not induce EAE when given in combination with GpSC but without BP. CFA combined with GpSC only occasionally induced EAE. However, EAE could be induced by the combination of CFA and GpSC or IFA and GpSC in a part of the animals tested if they had been pretreated or simultaneously been injected with living BCG by intravenous route. EAE could not be enhanced by the additional injection of VCN. Surprisingly, most of the animals peracutely died after injection of CFA and BP in combination with GpSC when they had been pretreated with CP. This effect was most pronounced when pretreatment was done on day -4. No acute effect could be seen when CP was given simultaneously to CFA, BP and GpSC. Animals which did not peracutely succumb developed EAE similarly as those in the positive control groups. CP treatment simultaneously with BP but without CFA resulted in a reduction of the EAE specific mortality. This reduction could not be seen if treatment with CP was done after injection of GpSC and BP. 相似文献
10.
Sarah A. Collier Li Deng Elizabeth A. Adam Katharine M. Benedict Elizabeth M. Beshearse Anna J. Blackstock Beau B. Bruce Gordana Derado Chris Edens Kathleen E. Fullerton Julia W. Gargano Aimee L. Geissler Aron J. Hall Arie H. Havelaar Vincent R. Hill Robert M. Hoekstra Sujan C. Reddy Elaine Scallan Erin K. Stokes Jonathan S. Yoder Michael J. Beach 《Emerging infectious diseases》2021,27(1):140
Provision of safe drinking water in the United States is a great public health achievement. However, new waterborne disease challenges have emerged (e.g., aging infrastructure, chlorine-tolerant and biofilm-related pathogens, increased recreational water use). Comprehensive estimates of the health burden for all water exposure routes (ingestion, contact, inhalation) and sources (drinking, recreational, environmental) are needed. We estimated total illnesses, emergency department (ED) visits, hospitalizations, deaths, and direct healthcare costs for 17 waterborne infectious diseases. About 7.15 million waterborne illnesses occur annually (95% credible interval [CrI] 3.88 million–12.0 million), results in 601,000 ED visits (95% CrI 364,000–866,000), 118,000 hospitalizations (95% CrI 86,800–150,000), and 6,630 deaths (95% CrI 4,520–8,870) and incurring US $3.33 billion (95% CrI 1.37 billion–8.77 billion) in direct healthcare costs. Otitis externa and norovirus infection were the most common illnesses. Most hospitalizations and deaths were caused by biofilm-associated pathogens (nontuberculous mycobacteria, Pseudomonas, Legionella), costing US $2.39 billion annually. 相似文献