Frequent Detection of Hepatitis B Virus X-Gene DNA in Hepatocellular Carcinoma and Adjacent Liver Tissue in Hepatitis B Surface Antigen-Negative Patients

Hepatitis B virus is associated with humanhepatocellular carcinoma. We performed polymerase chainreaction for the X, C, S, and preS2/S regions of theviral genome in 23 hepatitis B surface antigen-negative hepatocellular carcinomas and adjacent liver.Hepatitis B viral genomes were detected in 17 of 23tumors and adjacent tissues (73.9%). Among recognizedtransactivators, the X gene was present in 16 (69.6%) cases of hepatocellular carcinoma, but preS2/Swas detected in only 7 (30.4%). Hepatitis B virus C andS regions were detected in 3 (13.0%) and 9 (39.1%)hepatocellular carcinomas, respectively. Serologic study revealed antibodies to hepatitis Bsurface antigen, hepatitis B core antigen, and hepatitisB e antigen in 14 patients; among these, X-gene DNA wasdetected in 12 of 14 tumors (85.7%). The X gene was also detected in 4 of 9 tumors ofseronegative patients. The X gene, present in manyhepatocellular carcinomas, may promote hepatocellularcarcinoma in hepatitis B surface antigen-negativepatients.     

新余市从业人员乙型肝炎病毒标志物的检测分析

目的：了解从业人员HBV感染现状,进一步加强对HBSAG携带者的管理。方法：采用ELISA法,对新余地区从业人员进行HBSAG,HBEAG和抗HBCTGM检测。结果：2007年1月～2009年12月共检测16181人,其中HBSAG阳性1284人,阳性率为7.93%。在1284名HBSAG阳性者中HBEAG阳性者70人,阳性5.42%,其阳性随年龄递增而下降（X2=4.901675,P〈0.01）;抗-HBCIGM阳性者8人,阳性率0.62%,各年龄组间抗-HBCIGM的阳性率差异有统计学意义（X2=1.61）。在HBV血清标志物组合模式中,以HBSAG阳性＋HBEAG阴性＋抗-HBCIGM阴性所占比例最高,以HBSAG阳性＋HBEAG阳性＋抗-HBCIGM阳性所占比例最低。结论：新余地区从业人员属于HBV高流行人群,结果显示加强对从业人员的体检是关键。