ELABELA通过调控AKT信号通路影响滋养细胞侵袭的研究

目的 探讨ELABELA(ELA)对妊娠滋养细胞(Bewo细胞)侵袭行为的影响及其可能的作用机制。方法 用siRNA-ELA转染处于对数生长期的Bewo细胞,分为siRNA-ELA转染组,阴性对照组(细胞转染无意义序列)及空白组,细胞转染后于倒置显微镜下观察转染效率,采用实时荧光定量聚合酶链反应(qRT-PCR)检测转染后各组细胞中ELA mRNA表达并明确沉默效率。划痕实验测定各组细胞迁移能力,Transwell实验测定各组细胞的迁移、侵袭能力。蛋白质免疫印迹法检测各组细胞p-AKT、MMP9蛋白表达水平。结果 si-ELA组的ELA表达量明显低于si-NC组及空白组(P<0.001),且si-NC组与空白组差异无统计学意义(P>0.05);siRNA-ELA组细胞的迁移及侵袭能力均明显低于空白组(P<0.01;P<0.01);siRNA-ELA组p-AKT蛋白及MMP9蛋白表达水平明显低于空白组(p-AKT P<0.01;MMP9 P<0.001)。结论 敲低ELA可抑制滋养细胞的侵袭及迁移能力,其机制可能与AKT信号通路有关。     

Apelin、ELABELA分子及其共受体APJ在子痫前期中的研究进展

子痫前期的发病机制目前尚未完全阐明,但是胎盘的核心作用不可忽视。最近研究表明,Apelin、ELABELA及其共受体APJ在胎盘形成、血管发育及血管张力调控中发挥重要的作用。本文将针对Apelin、ELABELA分子及其共受体APJ的研究起源、结构、表达及其与子痫前期的关系做相关论述。     

Predicting late-onset preeclampsia by detecting ELABELA content using an immunochromatographic colloidal gold test strip

Preeclampsia is a pregnancy disorder that seriously affects the outcome of mothers and infants and lacks effective prediction and diagnosis methods. ELABELA is the second endogenous ligand of the apelin receptor (APJ) and is associated with the pathogenesis of preeclampsia. In a previous study, the authors found that the downregulation of ELABELA expression is closely related to late-onset preeclampsia, which may be a marker for the clinical diagnosis of late-onset preeclampsia. In this study, the authors again collected 120 maternal blood samples, including 60 pregnant women with a medical diagnosis of late-onset preeclampsia. ELISA results showed that the serum ELABELA concentration in late-onset preeclampsia pregnant women (12.57 ± 7.77 ng/mL) was significantly lower than that in normal pregnant women (36.99 ± 23.58 ng/mL), which was consistent with previously reported results. Therefore, the authors used an ELABELA monoclonal antibody to label four colloidal gold nanoparticles with different diameters (15, 30, 55, and 150 nm) and developed a transverse-flow immunochromatographic band for the rapid and accurate detection of serum ELABELA levels. The strip test shows that colloidal gold with a diameter of 30 nm can be used as a good ELABELA detection marker and had more than 90% positive detection effect. Therefore, the authors hope that the colloidal gold strip with ELABELA as the diagnostic index developed by us will be popularized and applied in clinical diagnosis.